O24509 (INVA_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Acid beta-fructofuranosidase EC=3.2.1.26 Alternative name(s): Acid invertase Short name=AI Acid sucrose hydrolase Vacuolar invertase
Organism	Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier	3885 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Vacuole Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological_process	sucrose metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Propeptide	37 – 101	65	By similarity		PRO_0000033389
	Chain	102 – 651	550	Acid beta-fructofuranosidase		PRO_0000033390
Regions
	Region	127 – 130	4	Substrate binding By similarity
	Region	189 – 190	2	Substrate binding By similarity
	Region	253 – 254	2	Substrate binding By similarity
Sites
	Active site	130	1	By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	308	1	Substrate By similarity
	Binding site	343	1	Substrate By similarity
Amino acid modifications
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	620	1	N-linked (GlcNAc...) Potential
	Disulfide bond	500 ↔ 548		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24509 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6303538073EFA479	FASTA	651	72,686
	10 20 30 40 50 60 MEHHKPLLPT SSHAAPNPRT RKDLLLLLCA LLFLSSLVAF GRNRASNVPH DHVSSSASNH 70 80 90 100 110 120 QQEHQSPTSL PSSKWHAVSR GVSSGVSEKS SSMLFSGEGG ASEAFPWDNS MLSWQRTSFH 130 140 150 160 170 180 FQPEKNWMND PNGPMYYKGW YHFFYQYNPN GAVWGDIVWG HAVSRDMIHW LHLPLAMVAD 190 200 210 220 230 240 QWYDKQGVWT GSATILPNGE IIMLYTGSTN ESVQVQNLAY PADPSDPLLV DWIKHPGNPV 250 260 270 280 290 300 LVPPPGIGAK DFRDPTTAWL TSEGKWRITI GSKLNKTGIA LVYDTDDFKT YELKNGHLRA 310 320 330 340 350 360 VPGTGMWECV DFFPVSKKNE NGLDTSLSIN GAEVKYVMKV SLDDDRHDYY TIGTYDENKV 370 380 390 400 410 420 LFTPDDVKND VGVGLRYDYG IFYASKTFYD QNMDRRILWG WIGESDSEYA DVTKGWASVQ 430 440 450 460 470 480 SIPRTVRLDK KTGSNLLQWP VAEVESLRLR SDEFKSLKAK PGSVVSLDIE TATQLDVVAE 490 500 510 520 530 540 FEIDAESLQK TAQSNEEFTC STSGGAAQRG ALGPFGLLVL ADEGLSEYTP VYFYVIKGRN 550 560 570 580 590 600 GNLKTSFCSD QSRSSQPNDV RKQIFGNIVP VLEGEKFSLR MLVDHSIVES FAQGGRTCVT 610 620 630 640 650 SRVYPTKAIY GAARLFLFNN ATEATVTASL KIWQMNSAFI RPFPFNPDQK N

References

[1]	Blee K.A., Anderson A.J. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92438 mRNA. Translation: AAB68679.1.
PIR	T12083.
3D structure databases
ProteinModelPortal	O24509.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00238.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR021792. Beta-fructofuranosidase. IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF11837. DUF3357. 1 hit. PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INVA_PHAVU

Accession

Primary (citable) accession number: O24509

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	March 6, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program