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Protein

Hydroxyacylglutathione hydrolase cytoplasmic

Gene

GLX2-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.3 Publications

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.2 Publications

Cofactori

Fe2+1 Publication, Zn2+1 Publication, Fe3+1 PublicationNote: Binds 1 Fe2+ or Fe3+ and 1 Zn2+ ion per subunit, catalysis is optimal with 1 Fe and 1 Zn. Electron spin resonance indicates the presence of a mixture of protein molecules that contain either Fe2+ or Fe3+ and Zn2+. Mn2+ is not a cofactor (PubMed:19834746).1 Publication

Kineticsi

  1. KM=220 µM for S-D-lactoylglutathion1 Publication

    Pathwayi: methylglyoxal degradation

    This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (AXX17_At1g07880), Lactoylglutathione lyase GLX1 (GLX1), Lactoylglutathione lyase (AXX17_At1g07880), Probable lactoylglutathione lyase, chloroplastic (At1g67280), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (AXX17_At1g12200)
    2. Probable hydroxyacylglutathione hydrolase 2, chloroplastic (GLX2-4), Hydroxyacylglutathione hydrolase cytoplasmic (GLX2-2), Hydroxyacylglutathione hydrolase 1, mitochondrial (GLX2-1), Hydroxyacylglutathione hydrolase 2, mitochondrial (At2g31350)
    This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi54Zinc; via tele nitrogenBy similarity1
    Metal bindingi56Zinc; via pros nitrogenBy similarity1
    Metal bindingi58IronBy similarity1
    Metal bindingi59Iron; via tele nitrogenBy similarity1
    Metal bindingi112Zinc; via tele nitrogenBy similarity1
    Metal bindingi135IronBy similarity1
    Metal bindingi135ZincBy similarity1
    Metal bindingi174Iron; via tele nitrogenBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandIron, Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.1.2.6 399
    ReactomeiR-ATH-70268 Pyruvate metabolism
    SABIO-RKiO24496
    UniPathwayiUPA00619; UER00676

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyacylglutathione hydrolase cytoplasmic (EC:3.1.2.62 Publications)
    Alternative name(s):
    Glyoxalase II
    Short name:
    Glx II
    Gene namesi
    Name:GLX2-2
    Synonyms:GLY2
    Ordered Locus Names:At3g10850
    ORF Names:T7M13.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G10850
    TAIRilocus:2103232 AT3G10850

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi54H → N: Binds normal amount of metal, but reduced enzyme activity. 1 Publication1
    Mutagenesisi58D → C: Binds normal amount of metal, but reduced enzyme activity. 1 Publication1
    Mutagenesisi142C → A: Increases the metal content and the enzyme activity. 1 Publication1
    Mutagenesisi144K → A: Binds normal amount of metal, but reduced enzyme activity. 1 Publication1
    Mutagenesisi180N → A: 70% reduction in enzyme activity. 1 Publication1
    Mutagenesisi227R → A: Decreases metal binding and enzyme stability. 1 Publication1
    Mutagenesisi250R → W: Decreases the substrate affinity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001923471 – 258Hydroxyacylglutathione hydrolase cytoplasmicAdd BLAST258

    Proteomic databases

    PaxDbiO24496

    PTM databases

    iPTMnetiO24496

    Expressioni

    Tissue specificityi

    Mainly expressed in flowers and flower buds. Also detected in roots and leaves.1 Publication

    Gene expression databases

    ExpressionAtlasiO24496 baseline and differential
    GenevisibleiO24496 AT

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Protein-protein interaction databases

    BioGridi5589, 3 interactors
    STRINGi3702.AT3G10850.1

    Structurei

    3D structure databases

    ProteinModelPortaliO24496
    SMRiO24496
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni144 – 146Substrate bindingBy similarity3
    Regioni174 – 176Substrate bindingBy similarity3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG0813 Eukaryota
    COG0491 LUCA
    HOGENOMiHOG000058041
    InParanoidiO24496
    KOiK01069
    OMAiNYIWLLQ
    OrthoDBiEOG09360IJQ
    PhylomeDBiO24496

    Family and domain databases

    CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
    Gene3Di3.60.15.10, 1 hit
    HAMAPiMF_01374 Glyoxalase_2, 1 hit
    InterProiView protein in InterPro
    IPR035680 Clx_II_MBL
    IPR032282 HAGH_C
    IPR017782 Hydroxyacylglutathione_Hdrlase
    IPR001279 Metallo-B-lactamas
    IPR036866 RibonucZ/Hydroxyglut_hydro
    PfamiView protein in Pfam
    PF16123 HAGH_C, 1 hit
    PF00753 Lactamase_B, 1 hit
    PIRSFiPIRSF005457 Glx, 1 hit
    SMARTiView protein in SMART
    SM00849 Lactamase_B, 1 hit
    SUPFAMiSSF56281 SSF56281, 1 hit
    TIGRFAMsiTIGR03413 GSH_gloB, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O24496-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIFHVPCLQ DNYSYLIIDE STGDAAVVDP VDPEKVIASA EKHQAKIKFV
    60 70 80 90 100
    LTTHHHWDHA GGNEKIKQLV PDIKVYGGSL DKVKGCTDAV DNGDKLTLGQ
    110 120 130 140 150
    DINILALHTP CHTKGHISYY VNGKEGENPA VFTGDTLFVA GCGKFFEGTA
    160 170 180 190 200
    EQMYQSLCVT LAALPKPTQV YCGHEYTVKN LEFALTVEPN NGKIQQKLAW
    210 220 230 240 250
    ARQQRQADLP TIPSTLEEEL ETNPFMRVDK PEIQEKLGCK SPIDTMREVR

    NKKDQWRG
    Length:258
    Mass (Da):28,792
    Last modified:December 15, 1998 - v2
    Checksum:i6703B98A8F902B5A
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti14S → T in AAC49867 (PubMed:9349270).Curated1
    Sequence conflicti85 – 93GCTDAVDNG → VALMRLIC in AAC49867 (PubMed:9349270).Curated9
    Sequence conflicti98 – 102LGQDI → WSGY in AAC49867 (PubMed:9349270).Curated5
    Sequence conflicti122N → T in AAC49867 (PubMed:9349270).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U90929 mRNA Translation: AAC49867.1
    Y08357 mRNA Translation: CAA69644.1
    AC011708 Genomic DNA Translation: AAF19564.1
    CP002686 Genomic DNA Translation: AEE74963.1
    AY052329 mRNA Translation: AAK96522.1
    BT000849 mRNA Translation: AAN38686.1
    RefSeqiNP_187696.1, NM_111922.4
    UniGeneiAt.47367
    At.69008

    Genome annotation databases

    EnsemblPlantsiAT3G10850.1; AT3G10850.1; AT3G10850
    GeneIDi820255
    GrameneiAT3G10850.1; AT3G10850.1; AT3G10850
    KEGGiath:AT3G10850

    Similar proteinsi

    Entry informationi

    Entry nameiGLO2C_ARATH
    AccessioniPrimary (citable) accession number: O24496
    Secondary accession number(s): O04844
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 15, 1998
    Last modified: April 25, 2018
    This is version 130 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health