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Protein

Hydroxyacylglutathione hydrolase 1, mitochondrial

Gene

GLX2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Fe2+By similarity, Fe3+By similarity, Zn2+By similarityNote: Binds 1 Fe2+ or Fe3+ and 1 Zn2+ ion per subunit. Electron spin resonance indicates the presence of a mixture of protein molecules that contain either Fe2+ or Zn2+.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (AXX17_At1g07880), Lactoylglutathione lyase GLX1 (GLX1), Lactoylglutathione lyase (AXX17_At1g07880), Probable lactoylglutathione lyase, chloroplastic (At1g67280), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (AXX17_At1g12200)
  2. Probable hydroxyacylglutathione hydrolase 2, chloroplastic (GLX2-4), Hydroxyacylglutathione hydrolase cytoplasmic (GLX2-2), Hydroxyacylglutathione hydrolase 1, mitochondrial (GLX2-1), Hydroxyacylglutathione hydrolase 2, mitochondrial (At2g31350)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Zinc; via tele nitrogenBy similarity1
Metal bindingi133Zinc; via pros nitrogenBy similarity1
Metal bindingi135IronBy similarity1
Metal bindingi136Iron; via tele nitrogenBy similarity1
Metal bindingi189Zinc; via tele nitrogenBy similarity1
Metal bindingi208IronBy similarity1
Metal bindingi208ZincBy similarity1

GO - Molecular functioni

  • beta-lactamase activity Source: TAIR
  • hydroxyacylglutathione hydrolase activity Source: UniProtKB-EC
  • metal ion binding Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • antibiotic catabolic process Source: InterPro
  • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: InterPro
  • response to amino acid Source: TAIR
  • response to anoxia Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase 1, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:GLX2-1
Synonyms:GLY1
Ordered Locus Names:At2g43430
ORF Names:T1O24.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43430
TAIRilocus:2058239 AT2G43430

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 76MitochondrionSequence analysisAdd BLAST76
ChainiPRO_000001228677 – 331Hydroxyacylglutathione hydrolase 1, mitochondrialAdd BLAST255

Proteomic databases

PaxDbiO24495
PRIDEiO24495

Expressioni

Tissue specificityi

Mainly expressed in roots, flowers and flower buds. Also detected in leaves.1 Publication

Gene expression databases

ExpressionAtlasiO24495 baseline and differential
GenevisibleiO24495 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G43430.1

Structurei

3D structure databases

ProteinModelPortaliO24495
SMRiO24495
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0813 Eukaryota
COG0491 LUCA
HOGENOMiHOG000058041
InParanoidiO24495
KOiK01069
OMAiVMWLLSM
OrthoDBiEOG09360H08
PhylomeDBiO24495

Family and domain databases

CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
Gene3Di3.60.15.10, 1 hit
HAMAPiMF_01374 Glyoxalase_2, 1 hit
InterProiView protein in InterPro
IPR001018 Beta-lactamase_class-B_CS
IPR035680 Clx_II_MBL
IPR032282 HAGH_C
IPR017782 Hydroxyacylglutathione_Hdrlase
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF16123 HAGH_C, 1 hit
PF00753 Lactamase_B, 1 hit
PIRSFiPIRSF005457 Glx, 1 hit
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit
TIGRFAMsiTIGR03413 GSH_gloB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O24495-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVISKASST TTNSSIPSCS RIGGQLCVWP GLRQLCLRKS LLYGVMWLLS
60 70 80 90 100
MPLKTLRGAR KTLKITHFCS ISNMPSSLKI ELVPCSKDNY AYLLHDEDTG
110 120 130 140 150
TVGVVDPSEA APVIEALSRK NWNLTYILNT HHHDDHIGGN AELKERYGAK
160 170 180 190 200
VIGSAVDKDR IPGIDILLKD SDKWMFAGHE VRILDTPGHT QGHISFYFPG
210 220 230 240 250
SATIFTGDLI YSLSCGTLSE GTPEQMLSSL QKIVSLPDDT NIYCGRENTA
260 270 280 290 300
GNLKFALSVE PKNETLQSYA TRVAHLRSQG LPSIPTTVKV EKACNPFLRI
310 320 330
SSKDIRKSLS IPDSATEAEA LRRIQRARDR F
Length:331
Mass (Da):36,500
Last modified:May 27, 2002 - v2
Checksum:i2EDC21B4902419C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159D → H in AAC49866 (PubMed:9349270).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90927 Genomic DNA Translation: AAC49865.1
U90928 mRNA Translation: AAC49866.1
AC002335 Genomic DNA Translation: AAB64315.2
CP002685 Genomic DNA Translation: AEC10268.1
AY091278 mRNA Translation: AAM14217.1
AY063806 mRNA Translation: AAL36162.1
PIRiA84866
RefSeqiNP_565999.1, NM_129904.3 [O24495-1]
UniGeneiAt.10817

Genome annotation databases

EnsemblPlantsiAT2G43430.1; AT2G43430.1; AT2G43430 [O24495-1]
GeneIDi818944
GrameneiAT2G43430.1; AT2G43430.1; AT2G43430 [O24495-1]
KEGGiath:AT2G43430

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLO2M_ARATH
AccessioniPrimary (citable) accession number: O24495
Secondary accession number(s): O22857, O24494
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 27, 2002
Last modified: April 25, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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