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Protein

Hydroxyacylglutathione hydrolase 1, mitochondrial

Gene

GLX2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Fe2+By similarity, Fe3+By similarity, Zn2+By similarityNote: Binds 1 Fe2+ or Fe3+ and 1 Zn2+ ion per subunit. Electron spin resonance indicates the presence of a mixture of protein molecules that contain either Fe2+ or Zn2+.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLX1), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g11840), Lactoylglutathione lyase (F12F1.32), Lactoylglutathione lyase (GLX1), Lactoylglutathione lyase (AXX17_At1g07880), Probable lactoylglutathione lyase, chloroplastic (At1g67280), Lactoylglutathione lyase (At1g08110), Lactoylglutathione lyase (At1g11840), Lactoylglutathione lyase (At1g08110)
  2. Hydroxyacylglutathione hydrolase cytoplasmic (GLX2-2), Hydroxyacylglutathione hydrolase 2, mitochondrial (At2g31350), Probable hydroxyacylglutathione hydrolase 2, chloroplastic (GLX2-4), Hydroxyacylglutathione hydrolase 1, mitochondrial (GLX2-1)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Zinc; via tele nitrogenBy similarity1
Metal bindingi133Zinc; via pros nitrogenBy similarity1
Metal bindingi135IronBy similarity1
Metal bindingi136Iron; via tele nitrogenBy similarity1
Metal bindingi189Zinc; via tele nitrogenBy similarity1
Metal bindingi208IronBy similarity1
Metal bindingi208ZincBy similarity1

GO - Molecular functioni

  • beta-lactamase activity Source: TAIR
  • hydroxyacylglutathione hydrolase activity Source: UniProtKB-EC
  • metal ion binding Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • antibiotic catabolic process Source: InterPro
  • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: InterPro
  • response to amino acid Source: TAIR
  • response to anoxia Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase 1, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:GLX2-1
Synonyms:GLY1
Ordered Locus Names:At2g43430
ORF Names:T1O24.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43430.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 76MitochondrionSequence analysisAdd BLAST76
ChainiPRO_000001228677 – 331Hydroxyacylglutathione hydrolase 1, mitochondrialAdd BLAST255

Proteomic databases

PaxDbiO24495.
PRIDEiO24495.

Expressioni

Tissue specificityi

Mainly expressed in roots, flowers and flower buds. Also detected in leaves.1 Publication

Gene expression databases

ExpressionAtlasiO24495. baseline and differential.
GenevisibleiO24495. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G43430.1.

Structurei

3D structure databases

ProteinModelPortaliO24495.
SMRiO24495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
HOGENOMiHOG000058041.
InParanoidiO24495.
KOiK01069.
OMAiHEYTKNN.
OrthoDBiEOG09360H08.
PhylomeDBiO24495.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR001018. Beta-lactamase_class-B_CS.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O24495-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVISKASST TTNSSIPSCS RIGGQLCVWP GLRQLCLRKS LLYGVMWLLS
60 70 80 90 100
MPLKTLRGAR KTLKITHFCS ISNMPSSLKI ELVPCSKDNY AYLLHDEDTG
110 120 130 140 150
TVGVVDPSEA APVIEALSRK NWNLTYILNT HHHDDHIGGN AELKERYGAK
160 170 180 190 200
VIGSAVDKDR IPGIDILLKD SDKWMFAGHE VRILDTPGHT QGHISFYFPG
210 220 230 240 250
SATIFTGDLI YSLSCGTLSE GTPEQMLSSL QKIVSLPDDT NIYCGRENTA
260 270 280 290 300
GNLKFALSVE PKNETLQSYA TRVAHLRSQG LPSIPTTVKV EKACNPFLRI
310 320 330
SSKDIRKSLS IPDSATEAEA LRRIQRARDR F
Length:331
Mass (Da):36,500
Last modified:May 27, 2002 - v2
Checksum:i2EDC21B4902419C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159D → H in AAC49866 (PubMed:9349270).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90927 Genomic DNA. Translation: AAC49865.1.
U90928 mRNA. Translation: AAC49866.1.
AC002335 Genomic DNA. Translation: AAB64315.2.
CP002685 Genomic DNA. Translation: AEC10268.1.
AY091278 mRNA. Translation: AAM14217.1.
AY063806 mRNA. Translation: AAL36162.1.
PIRiA84866.
RefSeqiNP_565999.1. NM_129904.3. [O24495-1]
UniGeneiAt.10817.

Genome annotation databases

EnsemblPlantsiAT2G43430.1; AT2G43430.1; AT2G43430. [O24495-1]
GeneIDi818944.
GrameneiAT2G43430.1; AT2G43430.1; AT2G43430.
KEGGiath:AT2G43430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90927 Genomic DNA. Translation: AAC49865.1.
U90928 mRNA. Translation: AAC49866.1.
AC002335 Genomic DNA. Translation: AAB64315.2.
CP002685 Genomic DNA. Translation: AEC10268.1.
AY091278 mRNA. Translation: AAM14217.1.
AY063806 mRNA. Translation: AAL36162.1.
PIRiA84866.
RefSeqiNP_565999.1. NM_129904.3. [O24495-1]
UniGeneiAt.10817.

3D structure databases

ProteinModelPortaliO24495.
SMRiO24495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G43430.1.

Proteomic databases

PaxDbiO24495.
PRIDEiO24495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43430.1; AT2G43430.1; AT2G43430. [O24495-1]
GeneIDi818944.
GrameneiAT2G43430.1; AT2G43430.1; AT2G43430.
KEGGiath:AT2G43430.

Organism-specific databases

TAIRiAT2G43430.

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
HOGENOMiHOG000058041.
InParanoidiO24495.
KOiK01069.
OMAiHEYTKNN.
OrthoDBiEOG09360H08.
PhylomeDBiO24495.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Miscellaneous databases

PROiO24495.

Gene expression databases

ExpressionAtlasiO24495. baseline and differential.
GenevisibleiO24495. AT.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR001018. Beta-lactamase_class-B_CS.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2M_ARATH
AccessioniPrimary (citable) accession number: O24495
Secondary accession number(s): O22857, O24494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.