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O24475

- TPSD3_ABIGR

UniProt

O24475 - TPSD3_ABIGR

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Protein

Pinene synthase, chloroplastic

Gene

ag3

Organism
Abies grandis (Grand fir) (Pinus grandis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis. A mixture of alpha- and beta-pinene is produced by this enzyme.1 Publication

Catalytic activityi

Geranyl-diphosphate = (-)-alpha-pinene + diphosphate.1 Publication
Geranyl diphosphate = (-)-beta-pinene + diphosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Mn2+By similarityNote: Binds 3 Mg(2+) or Mn(2+) ions per subunit.By similarity
  • K(+)

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi379 – 3791Magnesium or manganese 1By similarity
Metal bindingi379 – 3791Magnesium or manganese 2By similarity
Metal bindingi383 – 3831Magnesium or manganese 1By similarity
Metal bindingi383 – 3831Magnesium or manganese 2By similarity
Metal bindingi531 – 5311Magnesium or manganese 3By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. terpene synthase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12819.
UniPathwayiUPA00924.

Names & Taxonomyi

Protein namesi
Recommended name:
Pinene synthase, chloroplastic (EC:4.2.3.119, EC:4.2.3.120)
Alternative name(s):
(-)-(1S,5S)-pinene synthase
Agg-pin1
Beta-geraniolene synthase
Gene namesi
Name:ag3
Synonyms:AG3.18
OrganismiAbies grandis (Grand fir) (Pinus grandis)
Taxonomic identifieri46611 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636ChloroplastSequence AnalysisAdd
BLAST
Chaini37 – 628592Pinene synthase, chloroplasticPRO_0000033628Add
BLAST

Expressioni

Inductioni

By wounding.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi379 – 3835DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsd subfamily.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24475-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALVSTAPLA SKSCLHKSLI SSTHELKALS RTIPALGMSR RGKSITPSIS
60 70 80 90 100
MSSTTVVTDD GVRRRMGDFH SNLWDDDVIQ SLPTAYEEKS YLERAEKLIG
110 120 130 140 150
EVKNMFNSMS LEDGELMSPL NDLIQRLWIV DSLERLGIHR HFKDEIKSAL
160 170 180 190 200
DYVYSYWGEN GIGCGRESVV TDLNSTALGL RTLRLHGYPV SSDVFKAFKG
210 220 230 240 250
QNGQFSCSEN IQTDEEIRGV LNLFRASLIA FPGEKIMDEA EIFSTKYLKE
260 270 280 290 300
ALQKIPVSSL SREIGDVLEY GWHTYLPRLE ARNYIQVFGQ DTENTKSYVK
310 320 330 340 350
SKKLLELAKL EFNIFQSLQK RELESLVRWW KESGFPEMTF CRHRHVEYYT
360 370 380 390 400
LASCIAFEPQ HSGFRLGFAK TCHLITVLDD MYDTFGTVDE LELFTATMKR
410 420 430 440 450
WDPSSIDCLP EYMKGVYIAV YDTVNEMARE AEEAQGRDTL TYAREAWEAY
460 470 480 490 500
IDSYMQEARW IATGYLPSFD EYYENGKVSC GHRISALQPI LTMDIPFPDH
510 520 530 540 550
ILKEVDFPSK LNDLACAILR LRGDTRCYKA DRARGEEASS ISCYMKDNPG
560 570 580 590 600
VSEEDALDHI NAMISDVIKG LNWELLKPDI NVPISAKKHA FDIARAFHYG
610 620
YKYRDGYSVA NVETKSLVTR TLLESVPL
Length:628
Mass (Da):71,506
Last modified:January 1, 1998 - v1
Checksum:i23DBB788F3C8072C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031K → E in AAK83564. (PubMed:11404343)Curated
Sequence conflicti134 – 1341E → G in AAK83564. (PubMed:11404343)Curated
Sequence conflicti169 – 1691V → A in AAK83564. (PubMed:11404343)Curated
Sequence conflicti180 – 1801L → F in AAK83564. (PubMed:11404343)Curated
Sequence conflicti286 – 2861Q → H in AAK83564. (PubMed:11404343)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87909 mRNA. Translation: AAB71085.1.
AF326517 Genomic DNA. Translation: AAK83564.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87909 mRNA. Translation: AAB71085.1 .
AF326517 Genomic DNA. Translation: AAK83564.1 .

3D structure databases

ProteinModelPortali O24475.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00924 .
BioCyci MetaCyc:MONOMER-12819.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
    Bohlmann J., Steele C.L., Croteau R.B.
    J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CATALYTIC ACTIVITY, CHARACTERIZATION.
  2. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
    Trapp S.C., Croteau R.B.
    Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-628, NOMENCLATURE.
  3. "Plant terpenoid synthases: molecular biology and phylogenetic analysis."
    Bohlmann J., Meyer-Gauen G., Croteau R.B.
    Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

Entry informationi

Entry nameiTPSD3_ABIGR
AccessioniPrimary (citable) accession number: O24475
Secondary accession number(s): Q94FW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3