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O24475

- TPSD3_ABIGR

UniProt

O24475 - TPSD3_ABIGR

Protein

Pinene synthase, chloroplastic

Gene

ag3

Organism
Abies grandis (Grand fir) (Pinus grandis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis. A mixture of alpha- and beta-pinene is produced by this enzyme.1 Publication

    Catalytic activityi

    Geranyl-diphosphate = (-)-alpha-pinene + diphosphate.1 Publication
    Geranyl diphosphate = (-)-beta-pinene + diphosphate.1 Publication

    Cofactori

    Binds 3 magnesium or manganese ions per subunit.By similarity
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi379 – 3791Magnesium or manganese 1By similarity
    Metal bindingi379 – 3791Magnesium or manganese 2By similarity
    Metal bindingi383 – 3831Magnesium or manganese 1By similarity
    Metal bindingi383 – 3831Magnesium or manganese 2By similarity
    Metal bindingi531 – 5311Magnesium or manganese 3By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. terpene synthase activity Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12819.
    UniPathwayiUPA00924.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pinene synthase, chloroplastic (EC:4.2.3.119, EC:4.2.3.120)
    Alternative name(s):
    (-)-(1S,5S)-pinene synthase
    Agg-pin1
    Beta-geraniolene synthase
    Gene namesi
    Name:ag3
    Synonyms:AG3.18
    OrganismiAbies grandis (Grand fir) (Pinus grandis)
    Taxonomic identifieri46611 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636ChloroplastSequence AnalysisAdd
    BLAST
    Chaini37 – 628592Pinene synthase, chloroplasticPRO_0000033628Add
    BLAST

    Expressioni

    Inductioni

    By wounding.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO24475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi379 – 3835DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family. Tpsd subfamily.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24475-1 [UniParc]FASTAAdd to Basket

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    MALVSTAPLA SKSCLHKSLI SSTHELKALS RTIPALGMSR RGKSITPSIS    50
    MSSTTVVTDD GVRRRMGDFH SNLWDDDVIQ SLPTAYEEKS YLERAEKLIG 100
    EVKNMFNSMS LEDGELMSPL NDLIQRLWIV DSLERLGIHR HFKDEIKSAL 150
    DYVYSYWGEN GIGCGRESVV TDLNSTALGL RTLRLHGYPV SSDVFKAFKG 200
    QNGQFSCSEN IQTDEEIRGV LNLFRASLIA FPGEKIMDEA EIFSTKYLKE 250
    ALQKIPVSSL SREIGDVLEY GWHTYLPRLE ARNYIQVFGQ DTENTKSYVK 300
    SKKLLELAKL EFNIFQSLQK RELESLVRWW KESGFPEMTF CRHRHVEYYT 350
    LASCIAFEPQ HSGFRLGFAK TCHLITVLDD MYDTFGTVDE LELFTATMKR 400
    WDPSSIDCLP EYMKGVYIAV YDTVNEMARE AEEAQGRDTL TYAREAWEAY 450
    IDSYMQEARW IATGYLPSFD EYYENGKVSC GHRISALQPI LTMDIPFPDH 500
    ILKEVDFPSK LNDLACAILR LRGDTRCYKA DRARGEEASS ISCYMKDNPG 550
    VSEEDALDHI NAMISDVIKG LNWELLKPDI NVPISAKKHA FDIARAFHYG 600
    YKYRDGYSVA NVETKSLVTR TLLESVPL 628
    Length:628
    Mass (Da):71,506
    Last modified:January 1, 1998 - v1
    Checksum:i23DBB788F3C8072C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti103 – 1031K → E in AAK83564. (PubMed:11404343)Curated
    Sequence conflicti134 – 1341E → G in AAK83564. (PubMed:11404343)Curated
    Sequence conflicti169 – 1691V → A in AAK83564. (PubMed:11404343)Curated
    Sequence conflicti180 – 1801L → F in AAK83564. (PubMed:11404343)Curated
    Sequence conflicti286 – 2861Q → H in AAK83564. (PubMed:11404343)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87909 mRNA. Translation: AAB71085.1.
    AF326517 Genomic DNA. Translation: AAK83564.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87909 mRNA. Translation: AAB71085.1 .
    AF326517 Genomic DNA. Translation: AAK83564.1 .

    3D structure databases

    ProteinModelPortali O24475.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00924 .
    BioCyci MetaCyc:MONOMER-12819.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProi IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
      Bohlmann J., Steele C.L., Croteau R.B.
      J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CATALYTIC ACTIVITY, CHARACTERIZATION.
    2. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
      Trapp S.C., Croteau R.B.
      Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-628, NOMENCLATURE.
    3. "Plant terpenoid synthases: molecular biology and phylogenetic analysis."
      Bohlmann J., Meyer-Gauen G., Croteau R.B.
      Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

    Entry informationi

    Entry nameiTPSD3_ABIGR
    AccessioniPrimary (citable) accession number: O24475
    Secondary accession number(s): Q94FW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3