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O24475 (TPSD3_ABIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pinene synthase, chloroplastic
EC=4.2.3.119
EC=4.2.3.120
Alternative name(s):
(-)-(1S,5S)-pinene synthase
Agg-pin1
Beta-geraniolene synthase
Gene names
Name:ag3
Synonyms:AG3.18
OrganismAbies grandis (Grand fir) (Pinus grandis)
Taxonomic identifier46611 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Pinaceae › Abies

Protein attributes

Sequence length628 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis. A mixture of alpha- and beta-pinene is produced by this enzyme. Ref.3

Catalytic activity

Geranyl-diphosphate = (-)-alpha-pinene + diphosphate. Ref.1

Geranyl diphosphate = (-)-beta-pinene + diphosphate. Ref.1

Cofactor

Binds 3 magnesium or manganese ions per subunit By similarity.

Potassium.

Pathway

Terpene metabolism; oleoresin biosynthesis.

Subcellular location

Plastid › chloroplast.

Induction

By wounding. Ref.1

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Miscellaneous

The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

Sequence similarities

Belongs to the terpene synthase family. Tpsd subfamily.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

terpene synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Chloroplast Potential
Chain37 – 628592Pinene synthase, chloroplastic
PRO_0000033628

Regions

Motif379 – 3835DDXXD motif

Sites

Metal binding3791Magnesium or manganese 1 By similarity
Metal binding3791Magnesium or manganese 2 By similarity
Metal binding3831Magnesium or manganese 1 By similarity
Metal binding3831Magnesium or manganese 2 By similarity
Metal binding5311Magnesium or manganese 3 By similarity

Experimental info

Sequence conflict1031K → E in AAK83564. Ref.2
Sequence conflict1341E → G in AAK83564. Ref.2
Sequence conflict1691V → A in AAK83564. Ref.2
Sequence conflict1801L → F in AAK83564. Ref.2
Sequence conflict2861Q → H in AAK83564. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O24475 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 23DBB788F3C8072C

FASTA62871,506
        10         20         30         40         50         60 
MALVSTAPLA SKSCLHKSLI SSTHELKALS RTIPALGMSR RGKSITPSIS MSSTTVVTDD 

        70         80         90        100        110        120 
GVRRRMGDFH SNLWDDDVIQ SLPTAYEEKS YLERAEKLIG EVKNMFNSMS LEDGELMSPL 

       130        140        150        160        170        180 
NDLIQRLWIV DSLERLGIHR HFKDEIKSAL DYVYSYWGEN GIGCGRESVV TDLNSTALGL 

       190        200        210        220        230        240 
RTLRLHGYPV SSDVFKAFKG QNGQFSCSEN IQTDEEIRGV LNLFRASLIA FPGEKIMDEA 

       250        260        270        280        290        300 
EIFSTKYLKE ALQKIPVSSL SREIGDVLEY GWHTYLPRLE ARNYIQVFGQ DTENTKSYVK 

       310        320        330        340        350        360 
SKKLLELAKL EFNIFQSLQK RELESLVRWW KESGFPEMTF CRHRHVEYYT LASCIAFEPQ 

       370        380        390        400        410        420 
HSGFRLGFAK TCHLITVLDD MYDTFGTVDE LELFTATMKR WDPSSIDCLP EYMKGVYIAV 

       430        440        450        460        470        480 
YDTVNEMARE AEEAQGRDTL TYAREAWEAY IDSYMQEARW IATGYLPSFD EYYENGKVSC 

       490        500        510        520        530        540 
GHRISALQPI LTMDIPFPDH ILKEVDFPSK LNDLACAILR LRGDTRCYKA DRARGEEASS 

       550        560        570        580        590        600 
ISCYMKDNPG VSEEDALDHI NAMISDVIKG LNWELLKPDI NVPISAKKHA FDIARAFHYG 

       610        620 
YKYRDGYSVA NVETKSLVTR TLLESVPL

« Hide

References

[1]"Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
Bohlmann J., Steele C.L., Croteau R.B.
J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CATALYTIC ACTIVITY, CHARACTERIZATION.
[2]"Genomic organization of plant terpene synthases and molecular evolutionary implications."
Trapp S.C., Croteau R.B.
Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-628, NOMENCLATURE.
[3]"Plant terpenoid synthases: molecular biology and phylogenetic analysis."
Bohlmann J., Meyer-Gauen G., Croteau R.B.
Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U87909 mRNA. Translation: AAB71085.1.
AF326517 Genomic DNA. Translation: AAK83564.1.

3D structure databases

ProteinModelPortalO24475.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12819.
UniPathwayUPA00924.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 1 hit.
SSF48576. Terpenoid_synth. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPSD3_ABIGR
AccessionPrimary (citable) accession number: O24475
Secondary accession number(s): Q94FW0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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