Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O24474

- TPSD2_ABIGR

UniProt

O24474 - TPSD2_ABIGR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Myrcene synthase, chloroplastic

Gene

ag2

Organism
Abies grandis (Grand fir) (Pinus grandis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis.2 Publications

Catalytic activityi

Geranyl diphosphate = myrcene + diphosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Mn2+By similarityNote: Binds 3 Mg(2+) or Mn(2+) ions per subunit.By similarity
  • K(+)

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi378 – 3781Magnesium or manganese 1By similarity
Metal bindingi378 – 3781Magnesium or manganese 2By similarity
Metal bindingi382 – 3821Magnesium or manganese 1By similarity
Metal bindingi382 – 3821Magnesium or manganese 2By similarity
Metal bindingi530 – 5301Magnesium or manganese 3By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. myrcene synthase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12818.
UniPathwayiUPA00924.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrcene synthase, chloroplastic (EC:4.2.3.15)
Alternative name(s):
Aggmyr
Gene namesi
Name:ag2
Synonyms:AG2.2
OrganismiAbies grandis (Grand fir) (Pinus grandis)
Taxonomic identifieri46611 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252ChloroplastSequence AnalysisAdd
BLAST
Chaini53 – 627575Myrcene synthase, chloroplasticPRO_0000033627Add
BLAST

Expressioni

Inductioni

By wounding.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi378 – 3825DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsd subfamily.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24474-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALVSISPLA SKSCLRKSLI SSIHEHKPPY RTIPNLGMRR RGKSVTPSMS
60 70 80 90 100
ISLATAAPDD GVQRRIGDYH SNIWDDDFIQ SLSTPYGEPS YQERAERLIV
110 120 130 140 150
EVKKIFNSMY LDDGRLMSSF NDLMQRLWIV DSVERLGIAR HFKNEITSAL
160 170 180 190 200
DYVFRYWEEN GIGCGRDSIV TDLNSTALGF RTLRLHGYTV SPEVLKAFQD
210 220 230 240 250
QNGQFVCSPG QTEGEIRSVL NLYRASLIAF PGEKVMEEAE IFSTRYLKEA
260 270 280 290 300
LQKIPVSALS QEIKFVMEYG WHTNLPRLEA RNYIDTLEKD TSAWLNKNAG
310 320 330 340 350
KKLLELAKLE FNIFNSLQQK ELQYLLRWWK ESDLPKLTFA RHRHVEFYTL
360 370 380 390 400
ASCIAIDPKH SAFRLGFAKM CHLVTVLDDI YDTFGTIDEL ELFTSAIKRW
410 420 430 440 450
NSSEIEHLPE YMKCVYMVVF ETVNELTREA EKTQGRNTLN YVRKAWEAYF
460 470 480 490 500
DSYMEEAKWI SNGYLPMFEE YHENGKVSSA YRVATLQPIL TLNAWLPDYI
510 520 530 540 550
LKGIDFPSRF NDLASSFLRL RGDTRCYKAD RDRGEEASCI SCYMKDNPGS
560 570 580 590 600
TEEDALNHIN AMVNDIIKEL NWELLRSNDN IPMLAKKHAF DITRALHHLY
610 620
IYRDGFSVAN KETKKLVMET LLESMLF
Length:627
Mass (Da):72,479
Last modified:January 1, 1998 - v1
Checksum:i2E0DA492E0C971FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87908 mRNA. Translation: AAB71084.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87908 mRNA. Translation: AAB71084.1 .

3D structure databases

ProteinModelPortali O24474.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00924 .
BioCyci MetaCyc:MONOMER-12818.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
    Bohlmann J., Steele C.L., Croteau R.B.
    J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CHARACTERIZATION.
  2. "Plant terpenoid synthases: molecular biology and phylogenetic analysis."
    Bohlmann J., Meyer-Gauen G., Croteau R.B.
    Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
  3. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
    Trapp S.C., Croteau R.B.
    Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

Entry informationi

Entry nameiTPSD2_ABIGR
AccessioniPrimary (citable) accession number: O24474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3