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O24474 (TPSD2_ABIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myrcene synthase, chloroplastic

EC=4.2.3.15
Alternative name(s):
Aggmyr
Gene names
Name:ag2
Synonyms:AG2.2
OrganismAbies grandis (Grand fir) (Pinus grandis)
Taxonomic identifier46611 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Protein attributes

Sequence length627 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis. Ref.2 Ref.3

Catalytic activity

Geranyl diphosphate = myrcene + diphosphate.

Cofactor

Binds 3 magnesium or manganese ions per subunit By similarity.

Potassium.

Pathway

Terpene metabolism; oleoresin biosynthesis.

Subcellular location

Plastidchloroplast.

Induction

By wounding. Ref.1

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Miscellaneous

The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

Sequence similarities

Belongs to the terpene synthase family. Tpsd subfamily.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

myrcene synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5252Chloroplast Potential
Chain53 – 627575Myrcene synthase, chloroplastic
PRO_0000033627

Regions

Motif378 – 3825DDXXD motif

Sites

Metal binding3781Magnesium or manganese 1 By similarity
Metal binding3781Magnesium or manganese 2 By similarity
Metal binding3821Magnesium or manganese 1 By similarity
Metal binding3821Magnesium or manganese 2 By similarity
Metal binding5301Magnesium or manganese 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
O24474 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 2E0DA492E0C971FD

FASTA62772,479
        10         20         30         40         50         60 
MALVSISPLA SKSCLRKSLI SSIHEHKPPY RTIPNLGMRR RGKSVTPSMS ISLATAAPDD 

        70         80         90        100        110        120 
GVQRRIGDYH SNIWDDDFIQ SLSTPYGEPS YQERAERLIV EVKKIFNSMY LDDGRLMSSF 

       130        140        150        160        170        180 
NDLMQRLWIV DSVERLGIAR HFKNEITSAL DYVFRYWEEN GIGCGRDSIV TDLNSTALGF 

       190        200        210        220        230        240 
RTLRLHGYTV SPEVLKAFQD QNGQFVCSPG QTEGEIRSVL NLYRASLIAF PGEKVMEEAE 

       250        260        270        280        290        300 
IFSTRYLKEA LQKIPVSALS QEIKFVMEYG WHTNLPRLEA RNYIDTLEKD TSAWLNKNAG 

       310        320        330        340        350        360 
KKLLELAKLE FNIFNSLQQK ELQYLLRWWK ESDLPKLTFA RHRHVEFYTL ASCIAIDPKH 

       370        380        390        400        410        420 
SAFRLGFAKM CHLVTVLDDI YDTFGTIDEL ELFTSAIKRW NSSEIEHLPE YMKCVYMVVF 

       430        440        450        460        470        480 
ETVNELTREA EKTQGRNTLN YVRKAWEAYF DSYMEEAKWI SNGYLPMFEE YHENGKVSSA 

       490        500        510        520        530        540 
YRVATLQPIL TLNAWLPDYI LKGIDFPSRF NDLASSFLRL RGDTRCYKAD RDRGEEASCI 

       550        560        570        580        590        600 
SCYMKDNPGS TEEDALNHIN AMVNDIIKEL NWELLRSNDN IPMLAKKHAF DITRALHHLY 

       610        620 
IYRDGFSVAN KETKKLVMET LLESMLF 

« Hide

References

[1]"Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
Bohlmann J., Steele C.L., Croteau R.B.
J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CHARACTERIZATION.
[2]"Plant terpenoid synthases: molecular biology and phylogenetic analysis."
Bohlmann J., Meyer-Gauen G., Croteau R.B.
Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[3]"Genomic organization of plant terpene synthases and molecular evolutionary implications."
Trapp S.C., Croteau R.B.
Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U87908 mRNA. Translation: AAB71084.1.

3D structure databases

ProteinModelPortalO24474.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12818.
UniPathwayUPA00924.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPSD2_ABIGR
AccessionPrimary (citable) accession number: O24474
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: March 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways