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O24474

- TPSD2_ABIGR

UniProt

O24474 - TPSD2_ABIGR

Protein

Myrcene synthase, chloroplastic

Gene

ag2

Organism
Abies grandis (Grand fir) (Pinus grandis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis.2 Publications

    Catalytic activityi

    Geranyl diphosphate = myrcene + diphosphate.

    Cofactori

    Binds 3 magnesium or manganese ions per subunit.By similarity
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi378 – 3781Magnesium or manganese 1By similarity
    Metal bindingi378 – 3781Magnesium or manganese 2By similarity
    Metal bindingi382 – 3821Magnesium or manganese 1By similarity
    Metal bindingi382 – 3821Magnesium or manganese 2By similarity
    Metal bindingi530 – 5301Magnesium or manganese 3By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. myrcene synthase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12818.
    UniPathwayiUPA00924.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrcene synthase, chloroplastic (EC:4.2.3.15)
    Alternative name(s):
    Aggmyr
    Gene namesi
    Name:ag2
    Synonyms:AG2.2
    OrganismiAbies grandis (Grand fir) (Pinus grandis)
    Taxonomic identifieri46611 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252ChloroplastSequence AnalysisAdd
    BLAST
    Chaini53 – 627575Myrcene synthase, chloroplasticPRO_0000033627Add
    BLAST

    Expressioni

    Inductioni

    By wounding.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO24474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi378 – 3825DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family. Tpsd subfamily.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24474-1 [UniParc]FASTAAdd to Basket

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    MALVSISPLA SKSCLRKSLI SSIHEHKPPY RTIPNLGMRR RGKSVTPSMS    50
    ISLATAAPDD GVQRRIGDYH SNIWDDDFIQ SLSTPYGEPS YQERAERLIV 100
    EVKKIFNSMY LDDGRLMSSF NDLMQRLWIV DSVERLGIAR HFKNEITSAL 150
    DYVFRYWEEN GIGCGRDSIV TDLNSTALGF RTLRLHGYTV SPEVLKAFQD 200
    QNGQFVCSPG QTEGEIRSVL NLYRASLIAF PGEKVMEEAE IFSTRYLKEA 250
    LQKIPVSALS QEIKFVMEYG WHTNLPRLEA RNYIDTLEKD TSAWLNKNAG 300
    KKLLELAKLE FNIFNSLQQK ELQYLLRWWK ESDLPKLTFA RHRHVEFYTL 350
    ASCIAIDPKH SAFRLGFAKM CHLVTVLDDI YDTFGTIDEL ELFTSAIKRW 400
    NSSEIEHLPE YMKCVYMVVF ETVNELTREA EKTQGRNTLN YVRKAWEAYF 450
    DSYMEEAKWI SNGYLPMFEE YHENGKVSSA YRVATLQPIL TLNAWLPDYI 500
    LKGIDFPSRF NDLASSFLRL RGDTRCYKAD RDRGEEASCI SCYMKDNPGS 550
    TEEDALNHIN AMVNDIIKEL NWELLRSNDN IPMLAKKHAF DITRALHHLY 600
    IYRDGFSVAN KETKKLVMET LLESMLF 627
    Length:627
    Mass (Da):72,479
    Last modified:January 1, 1998 - v1
    Checksum:i2E0DA492E0C971FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87908 mRNA. Translation: AAB71084.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87908 mRNA. Translation: AAB71084.1 .

    3D structure databases

    ProteinModelPortali O24474.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00924 .
    BioCyci MetaCyc:MONOMER-12818.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProi IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
      Bohlmann J., Steele C.L., Croteau R.B.
      J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CHARACTERIZATION.
    2. "Plant terpenoid synthases: molecular biology and phylogenetic analysis."
      Bohlmann J., Meyer-Gauen G., Croteau R.B.
      Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
    3. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
      Trapp S.C., Croteau R.B.
      Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

    Entry informationi

    Entry nameiTPSD2_ABIGR
    AccessioniPrimary (citable) accession number: O24474
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3