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O24474

- TPSD2_ABIGR

UniProt

O24474 - TPSD2_ABIGR

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Protein
Myrcene synthase, chloroplastic
Gene
ag2, AG2.2
Organism
Abies grandis (Grand fir) (Pinus grandis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis.2 Publications

Catalytic activityi

Geranyl diphosphate = myrcene + diphosphate.

Cofactori

Binds 3 magnesium or manganese ions per subunit By similarity.
Potassium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi378 – 3781Magnesium or manganese 1 By similarity
Metal bindingi378 – 3781Magnesium or manganese 2 By similarity
Metal bindingi382 – 3821Magnesium or manganese 1 By similarity
Metal bindingi382 – 3821Magnesium or manganese 2 By similarity
Metal bindingi530 – 5301Magnesium or manganese 3 By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. myrcene synthase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12818.
UniPathwayiUPA00924.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrcene synthase, chloroplastic (EC:4.2.3.15)
Alternative name(s):
Aggmyr
Gene namesi
Name:ag2
Synonyms:AG2.2
OrganismiAbies grandis (Grand fir) (Pinus grandis)
Taxonomic identifieri46611 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252Chloroplast Reviewed prediction
Add
BLAST
Chaini53 – 627575Myrcene synthase, chloroplastic
PRO_0000033627Add
BLAST

Expressioni

Inductioni

By wounding.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24474.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi378 – 3825DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24474-1 [UniParc]FASTAAdd to Basket

« Hide

MALVSISPLA SKSCLRKSLI SSIHEHKPPY RTIPNLGMRR RGKSVTPSMS    50
ISLATAAPDD GVQRRIGDYH SNIWDDDFIQ SLSTPYGEPS YQERAERLIV 100
EVKKIFNSMY LDDGRLMSSF NDLMQRLWIV DSVERLGIAR HFKNEITSAL 150
DYVFRYWEEN GIGCGRDSIV TDLNSTALGF RTLRLHGYTV SPEVLKAFQD 200
QNGQFVCSPG QTEGEIRSVL NLYRASLIAF PGEKVMEEAE IFSTRYLKEA 250
LQKIPVSALS QEIKFVMEYG WHTNLPRLEA RNYIDTLEKD TSAWLNKNAG 300
KKLLELAKLE FNIFNSLQQK ELQYLLRWWK ESDLPKLTFA RHRHVEFYTL 350
ASCIAIDPKH SAFRLGFAKM CHLVTVLDDI YDTFGTIDEL ELFTSAIKRW 400
NSSEIEHLPE YMKCVYMVVF ETVNELTREA EKTQGRNTLN YVRKAWEAYF 450
DSYMEEAKWI SNGYLPMFEE YHENGKVSSA YRVATLQPIL TLNAWLPDYI 500
LKGIDFPSRF NDLASSFLRL RGDTRCYKAD RDRGEEASCI SCYMKDNPGS 550
TEEDALNHIN AMVNDIIKEL NWELLRSNDN IPMLAKKHAF DITRALHHLY 600
IYRDGFSVAN KETKKLVMET LLESMLF 627
Length:627
Mass (Da):72,479
Last modified:January 1, 1998 - v1
Checksum:i2E0DA492E0C971FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87908 mRNA. Translation: AAB71084.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87908 mRNA. Translation: AAB71084.1 .

3D structure databases

ProteinModelPortali O24474.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00924 .
BioCyci MetaCyc:MONOMER-12818.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase."
    Bohlmann J., Steele C.L., Croteau R.B.
    J. Biol. Chem. 272:21784-21792(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CHARACTERIZATION.
  2. "Plant terpenoid synthases: molecular biology and phylogenetic analysis."
    Bohlmann J., Meyer-Gauen G., Croteau R.B.
    Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
  3. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
    Trapp S.C., Croteau R.B.
    Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

Entry informationi

Entry nameiTPSD2_ABIGR
AccessioniPrimary (citable) accession number: O24474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: March 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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