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Protein

Receptor for activated C kinase 1A

Gene

RACK1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major component of the RACK1 regulatory proteins that play a role in multiple signal transduction pathways. Involved in multiple hormone responses and developmental processes (PubMed:16829549, PubMed:18715992, PubMed:18947417). MAPK cascade scaffolding protein involved in the protease IV and ArgC signaling pathway but not the flg22 pathway (PubMed:25731164).4 Publications

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: UniProtKB
  • protein complex scaffold Source: TAIR
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • cellular response to abscisic acid stimulus Source: TAIR
  • gibberellin mediated signaling pathway Source: TAIR
  • positive regulation of signal transduction Source: UniProtKB
  • regulation of translation Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to gibberellin Source: TAIR
  • response to glucose Source: TAIR
  • ribosome biogenesis Source: TAIR
  • seed germination Source: TAIR
  • vegetative to reproductive phase transition of meristem Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor for activated C kinase 1A1 Publication
Alternative name(s):
Guanine nucleotide-binding protein subunit beta-like protein A
WD-40 repeat auxin-dependent protein ARCA
Gene namesi
Name:RACK1A1 Publication
Synonyms:ARCA1 Publication
Ordered Locus Names:At1g18080Imported
ORF Names:T10F20.9Imported, T10O22.6Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G18080.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Detected in the cytoplasm and nucleus when interacting with NUDT7.

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Shorter hypocotyls in etiolated seedlings, epinastic cotyledons, reduced rosette leaf production by half and late flowering under short-day conditions. Reduced sensitivity to gibberellin and brassinosteroid in seed germination, hypersensitivity to abscisic acid in seed germination and early seedling development, and hyposensitivity to auxin in adventitious and lateral root formation. Plants show a significant resistance to water stress conditions by limiting water loss through the guard cells.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277481 – 327Receptor for activated C kinase 1AAdd BLAST327

Proteomic databases

PaxDbiO24456.
PRIDEiO24456.

PTM databases

iPTMnetiO24456.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

GenevisibleiO24456. AT.

Interactioni

Subunit structurei

Homodimer and heterodimer with RACK1B or RACK1C (Probable). Interacts with NUDT7 (PubMed:22068106). Interacts with GB1, MEKK1, MKK4, MKK5, MPK3 and MPK6, but not with GPA1 or MPK4 (PubMed:25731164).Curated2 Publications

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: UniProtKB
  • protein complex scaffold Source: TAIR

Protein-protein interaction databases

BioGridi23627. 94 interactors.
IntActiO24456. 1 interactor.
STRINGi3702.AT1G18080.1.

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 12Combined sources9
Beta strandi18 – 22Combined sources5
Beta strandi29 – 35Combined sources7
Beta strandi38 – 44Combined sources7
Beta strandi54 – 60Combined sources7
Beta strandi66 – 71Combined sources6
Beta strandi75 – 82Combined sources8
Beta strandi85 – 91Combined sources7
Turni92 – 95Combined sources4
Beta strandi96 – 102Combined sources7
Beta strandi108 – 113Combined sources6
Beta strandi120 – 124Combined sources5
Beta strandi129 – 132Combined sources4
Beta strandi138 – 142Combined sources5
Beta strandi153 – 158Combined sources6
Beta strandi162 – 164Combined sources3
Beta strandi166 – 171Combined sources6
Beta strandi176 – 180Combined sources5
Turni181 – 183Combined sources3
Beta strandi186 – 190Combined sources5
Beta strandi197 – 202Combined sources6
Beta strandi206 – 213Combined sources8
Beta strandi219 – 222Combined sources4
Turni223 – 226Combined sources4
Beta strandi227 – 230Combined sources4
Beta strandi238 – 243Combined sources6
Beta strandi245 – 254Combined sources10
Beta strandi257 – 262Combined sources6
Turni263 – 266Combined sources4
Beta strandi267 – 272Combined sources6
Beta strandi298 – 303Combined sources6
Beta strandi307 – 314Combined sources8
Beta strandi317 – 323Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DM0X-ray2.40A1-327[»]
ProteinModelPortaliO24456.
SMRiO24456.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24456.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati13 – 44WD 1Add BLAST32
Repeati61 – 91WD 2Add BLAST31
Repeati103 – 133WD 3Add BLAST31
Repeati148 – 180WD 4Add BLAST33
Repeati192 – 222WD 5Add BLAST31
Repeati233 – 262WD 6Add BLAST30
Repeati293 – 323WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0279. Eukaryota.
ENOG410XQGZ. LUCA.
HOGENOMiHOG000091643.
InParanoidiO24456.
KOiK14753.
OMAiRQFISHT.
OrthoDBiEOG09360EGR.
PhylomeDBiO24456.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O24456-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGLVLKGT MRAHTDMVTA IATPIDNADI IVSASRDKSI ILWKLTKDDK
60 70 80 90 100
AYGVAQRRLT GHSHFVEDVV LSSDGQFALS GSWDGELRLW DLAAGVSTRR
110 120 130 140 150
FVGHTKDVLS VAFSLDNRQI VSASRDRTIK LWNTLGECKY TISEGGEGHR
160 170 180 190 200
DWVSCVRFSP NTLQPTIVSA SWDKTVKVWN LSNCKLRSTL AGHTGYVSTV
210 220 230 240 250
AVSPDGSLCA SGGKDGVVLL WDLAEGKKLY SLEANSVIHA LCFSPNRYWL
260 270 280 290 300
CAATEHGIKI WDLESKSIVE DLKVDLKAEA EKADNSGPAA TKRKVIYCTS
310 320
LNWSADGSTL FSGYTDGVIR VWGIGRY
Length:327
Mass (Da):35,748
Last modified:June 6, 2002 - v2
Checksum:i9DA103C4300FE96B
GO
Isoform 2 (identifier: O24456-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     195-245: Missing.

Note: No experimental confirmation available.
Show »
Length:276
Mass (Da):30,540
Checksum:i32E8BF614B517245
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178V → M in AAB82647 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040397195 – 245Missing in isoform 2. 1 PublicationAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77381 mRNA. Translation: AAB82647.1.
AC034107 Genomic DNA. Translation: AAF97825.1.
AC069551 Genomic DNA. Translation: AAF78369.1.
CP002684 Genomic DNA. Translation: AEE29673.1.
AY035007 mRNA. Translation: AAK59512.1.
AY063016 mRNA. Translation: AAL34190.1.
AK318781 mRNA. Translation: BAH56896.1.
AY088480 mRNA. Translation: AAM66016.1.
RefSeqiNP_173248.1. NM_101670.3. [O24456-1]
UniGeneiAt.22612.
At.67882.

Genome annotation databases

EnsemblPlantsiAT1G18080.1; AT1G18080.1; AT1G18080. [O24456-1]
GeneIDi838388.
GrameneiAT1G18080.1; AT1G18080.1; AT1G18080.
KEGGiath:AT1G18080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77381 mRNA. Translation: AAB82647.1.
AC034107 Genomic DNA. Translation: AAF97825.1.
AC069551 Genomic DNA. Translation: AAF78369.1.
CP002684 Genomic DNA. Translation: AEE29673.1.
AY035007 mRNA. Translation: AAK59512.1.
AY063016 mRNA. Translation: AAL34190.1.
AK318781 mRNA. Translation: BAH56896.1.
AY088480 mRNA. Translation: AAM66016.1.
RefSeqiNP_173248.1. NM_101670.3. [O24456-1]
UniGeneiAt.22612.
At.67882.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DM0X-ray2.40A1-327[»]
ProteinModelPortaliO24456.
SMRiO24456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23627. 94 interactors.
IntActiO24456. 1 interactor.
STRINGi3702.AT1G18080.1.

PTM databases

iPTMnetiO24456.

Proteomic databases

PaxDbiO24456.
PRIDEiO24456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G18080.1; AT1G18080.1; AT1G18080. [O24456-1]
GeneIDi838388.
GrameneiAT1G18080.1; AT1G18080.1; AT1G18080.
KEGGiath:AT1G18080.

Organism-specific databases

TAIRiAT1G18080.

Phylogenomic databases

eggNOGiKOG0279. Eukaryota.
ENOG410XQGZ. LUCA.
HOGENOMiHOG000091643.
InParanoidiO24456.
KOiK14753.
OMAiRQFISHT.
OrthoDBiEOG09360EGR.
PhylomeDBiO24456.

Miscellaneous databases

EvolutionaryTraceiO24456.
PROiO24456.

Gene expression databases

GenevisibleiO24456. AT.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBLPA_ARATH
AccessioniPrimary (citable) accession number: O24456
Secondary accession number(s): C0Z2G7, Q9LDI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 6, 2002
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.