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Protein

Ubiquitin carboxyl-terminal hydrolase 3

Gene

UBP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Required for the correct development of pollen.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei32Nucleophile1
Active sitei315Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.008

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 3 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 3
Short name:
AtUBP3
Ubiquitin thioesterase 3
Ubiquitin-specific-processing protease 3
Gene namesi
Name:UBP3
Ordered Locus Names:At4g39910
ORF Names:T5J17.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G39910
TAIRilocus:2140074 AT4G39910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000806942 – 371Ubiquitin carboxyl-terminal hydrolase 3Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiO24454
PRIDEiO24454

PTM databases

iPTMnetiO24454

Expressioni

Tissue specificityi

Constitutively and ubiquitously expressed.1 Publication

Gene expression databases

ExpressionAtlasiO24454 baseline and differential
GenevisibleiO24454 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G39910.1

Structurei

3D structure databases

ProteinModelPortaliO24454
SMRiO24454
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 367USPAdd BLAST345

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi83 – 100Bipartite nuclear localization signalSequence analysisAdd BLAST18

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1864 Eukaryota
ENOG410XQ81 LUCA
HOGENOMiHOG000231498
InParanoidiO24454
KOiK11842
OMAiSYKAQQK
OrthoDBiEOG09360BZB
PhylomeDBiO24454

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAAGSKLEK ALGDQFPEGE RYFGFENFGN TCYCNSVLQA LYFCVPFREQ
60 70 80 90 100
LLEYYTSNKS VADAEENLMT CLADLFSQIS SQKKKTGVIA PKRFVQRLKK
110 120 130 140 150
QNELFRSYMH QDAHEFLNYL LNEVVDILEK EAKATKTEHE TSSSSSPEKI
160 170 180 190 200
ANGLKVPQAN GVVHKEPIVT WVHNIFQGIL TNETRCLRCE TVTARDETFL
210 220 230 240 250
DLSLDIEQNS SITSCLKNFS STETLHAEDK FFCDKCCSLQ EAQKRMKIKK
260 270 280 290 300
PPHILVIHLK RFKYIEQLGR YKKLSYRVVF PLELKLSNTV EPYADVEYSL
310 320 330 340 350
FAVVVHVGSG PNHGHYVSLV KSHNHWLFFD DENVEMIEES AVQTFFGSSQ
360 370
EYSSNTDHGY ILFYESLGPT K
Length:371
Mass (Da):42,447
Last modified:January 1, 1998 - v1
Checksum:i2C843280BDF24D3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76845 mRNA Translation: AAB67966.1
AL035708 Genomic DNA Translation: CAB38904.1
AL161596 Genomic DNA Translation: CAB80654.1
CP002687 Genomic DNA Translation: AEE87136.1
AY058889 mRNA Translation: AAL24275.1
AY079044 mRNA Translation: AAL79594.1
PIRiT06097
RefSeqiNP_568074.1, NM_120154.5
UniGeneiAt.24733

Genome annotation databases

EnsemblPlantsiAT4G39910.1; AT4G39910.1; AT4G39910
GeneIDi830150
GrameneiAT4G39910.1; AT4G39910.1; AT4G39910
KEGGiath:AT4G39910

Similar proteinsi

Entry informationi

Entry nameiUBP3_ARATH
AccessioniPrimary (citable) accession number: O24454
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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