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Protein

Pectate lyase

Gene

PL

Organism
Fragaria ananassa (Strawberry)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.UniRule annotation

Cofactori

Ca2+UniRule annotationNote: Binds 1 Ca2+ ion. Required for its activity.UniRule annotation

Pathwayi: pectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase, Pectinesterase (PE4), Pectinesterase, Pectinesterase (PE3), Pectinesterase (PE2), Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase, Pectinesterase (PE1), Pectinesterase, Pectinesterase
  2. Pectate lyase (PL), Pectate lyase (PL), Pectate lyase (PL), Pectate lyase (PL), Pectate lyase (PL), Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Keywords - Ligandi

CalciumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyaseUniRule annotation (EC:4.2.2.2UniRule annotation)
Gene namesi
Name:PLImported
OrganismiFragaria ananassa (Strawberry)Imported
Taxonomic identifieri3747 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeRosoideaePotentilleaeFragariinaeFragaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929UniRule annotationAdd
BLAST
Chaini30 – 405376Pectate lyaseUniRule annotationPRO_5007364447Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini175 – 372198Amb_allInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polysaccharide lyase 1 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

O24416-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLASSIATM HFYMTPLLLL LALLVCVSAS VENGKPVQSR FVEVVEEPRS
60 70 80 90 100
SFNSSMADRS NDHWNEHAVD NPEEIASLVD TSIRNSSTRR ELGYFSCATG
110 120 130 140 150
NPIDDCWRCD PQWQRHRKRP ANCGIGFGRN AVGGRDGKYY VVSDPGHDDP
160 170 180 190 200
VNPRPGTLRH AVIQDRPLWI VFKRDMVITL KQELIMNSFK TIDARGVNVH
210 220 230 240 250
IAYGGCITIQ FVTNVIIHGL HIHDCKPTGN AMVRSSPSHY GWRTMADGDG
260 270 280 290 300
ISIFGSSHIW VDHNSLSNCA DGLIDAIMGS TAITISNNYF THHNEVMLLG
310 320 330 340 350
HSDSYTRDKQ MQVTIAYNHF GEGLIQRMPR CRHGYFHVVN NDYTHWEMYA
360 370 380 390 400
IGGSADPTIN SQGNRYLAPN NRFAKEVTHR VQTTGRWRHW NWRSEGDLLL

NGAYF
Length:405
Mass (Da):45,745
Last modified:January 1, 1998 - v1
Checksum:i563BC01BCA640599
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63550 mRNA. Translation: AAB71208.1.
DQ076243 mRNA. Translation: AAZ31567.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63550 mRNA. Translation: AAB71208.1.
DQ076243 mRNA. Translation: AAZ31567.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plants."
    Medina-Escobar N., Cardenas J., Moyano E., Caballero J.L., Munoz-Blanco J.
    Plant Mol. Biol. 34:867-877(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ChandlerImported.
  2. "Polymorphism and influence of pectate lyase in strawberry fruit softening."
    Draye M.N., Van Cutsem P.J.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Fruit receptacleImported.

Entry informationi

Entry nameiO24416_FRAAN
AccessioniPrimary (citable) accession number: O24416
Entry historyi
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.