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Protein

Linoleate 9S-lipoxygenase 2

Gene

LOX1.2

Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Linoleic acid is the preferred substrate, but is also active with linolenic and arachidonic acids.PROSITE-ProRule annotation1 Publication

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.1 Publication

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

pH dependencei

Optimum pH is 6.0.1 Publication

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi522 – 5221Iron; catalyticPROSITE-ProRule annotation
Metal bindingi527 – 5271Iron; catalyticPROSITE-ProRule annotation
Metal bindingi713 – 7131Iron; catalyticPROSITE-ProRule annotation
Metal bindingi717 – 7171Iron; catalyticPROSITE-ProRule annotation
Metal bindingi861 – 8611Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12726.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Linoleate 9S-lipoxygenase 2 (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase 1-2
Gene namesi
Name:LOX1.2
Synonyms:LOX1, T8
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Linoleate 9S-lipoxygenase 2PRO_0000412920Add
BLAST

Expressioni

Tissue specificityi

Highly expressed in tubers and roots. Detected in flower buds and leaves.1 Publication

Developmental stagei

Expressed in stolons and increases during early stages of tuber development.1 Publication

Inductioni

Up-regulated in roots 2 hours after jasmonate treatment and 24 hours after wounding. Not induced in the leaves.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400054145.

Structurei

3D structure databases

ProteinModelPortaliO24379.
SMRiO24379. Positions 18-861.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 160132PLATPROSITE-ProRule annotationAdd
BLAST
Domaini163 – 861699LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi446 – 4516Poly-Thr

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiO24379.
KOiK15718.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGQITSGLF GGPDDSKKLK GTVVMMNKNA LDFTDLAGSL TDKAFEFLGQ
60 70 80 90 100
TVSFQLISSV QGDPTNGLQG KHSNPAYLEN SLFTLTPLTA GSETAFGVTF
110 120 130 140 150
DWNEEFGVPG AFIIKNTHIN EFFLKSLTLE DVPNHGKVHF VCNSWVYPSF
160 170 180 190 200
RYKSDRIFFV NQPYLPSKTP ELLRKYRENE LLTLRGDGTG KREAWDRIYD
210 220 230 240 250
YDIYNDLGNP DEGKENVRTT LGGSAEYPYP RRGRTGRPPT RTDPKSESRI
260 270 280 290 300
PLILSLDIYV PRDERFGHLK MSDFLTYALK SIVQFILPEL HALFDGTPNE
310 320 330 340 350
FDSFEDVLRL YEGGIKLPQG PLFKALTAAI PLEMIRELLR TDGEGILRFP
360 370 380 390 400
TPLVIKDSKT AWRTDEEFAR EMLAGVNPVI ISRLQEFPPK SKLDPEAYGN
410 420 430 440 450
QNSTITAEHI EDKLDGLTVD EAMNNNKLFI LNHHDVLIPY LRRINTTTTK
460 470 480 490 500
TYASRTLLFL QDNGSLKPLA IELSLPHPDG DQFGVTSKVY TPSDQGVESS
510 520 530 540 550
IWQLAKAYVA VNDSGVHQLI SHWLNTHAVI EPFVIATNRQ LSVLHPIHKL
560 570 580 590 600
LYPHFRDTMN INAMARQILI NAGGVLESTV FQSKFAMEMS AVVYKDWVFP
610 620 630 640 650
DQALPADLVK RGVAVEDSSS PHGVRLLIED YPYAVDGLEI WSAIKSWVSD
660 670 680 690 700
YCSFYYGSDE EILKDNELQA WWKELREVGH GDKKNEPWWP EMERPQELID
710 720 730 740 750
SCTTIIWIAS ALHAAVNFGQ YPYAGYLPNR PTVSRRFMPE PGTPEYEELK
760 770 780 790 800
KNPDKAFLKT ITAQLQTLLG VSLIEILSRH TTDEIYLGQR ESPEWTKDKE
810 820 830 840 850
PLAAFDKFGK KLTDIEKQII QRNGDNILTN RSGPVNAPYT LLFPTSEGGL
860
TGKGIPNSVS I
Length:861
Mass (Da):97,067
Last modified:January 1, 1998 - v1
Checksum:i25783F32C69BFA26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95513 mRNA. Translation: CAA64766.1.
RefSeqiNP_001275357.1. NM_001288428.1.
UniGeneiStu.965.

Genome annotation databases

GeneIDi102602192.
KEGGisot:102602192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95513 mRNA. Translation: CAA64766.1.
RefSeqiNP_001275357.1. NM_001288428.1.
UniGeneiStu.965.

3D structure databases

ProteinModelPortaliO24379.
SMRiO24379. Positions 18-861.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400054145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102602192.
KEGGisot:102602192.

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiO24379.
KOiK15718.

Enzyme and pathway databases

UniPathwayiUPA00382.
BioCyciMetaCyc:MONOMER-12726.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOX12_SOLTU
AccessioniPrimary (citable) accession number: O24379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.