O24379 (LOX12_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Linoleate 9S-lipoxygenase 2
EC=1.13.11.58

Alternative name(s):
Lipoxygenase 1-2

Gene names

Name:	LOX1.2
Synonyms:	LOX1, T8

Organism

Solanum tuberosum (Potato) [Reference proteome]

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Linoleic acid is the preferred substrate, but is also active with linolenic and arachidonic acids. Ref.1
Catalytic activity	Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate. Ref.1
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in tubers and roots. Detected in flower buds and leaves. Ref.1
Developmental stage	Expressed in stolons and increases during early stages of tuber development. Ref.1
Induction	Up-regulated in roots 2 hours after jasmonate treatment and 24 hours after wounding. Not induced in the leaves. Ref.1
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0. Ref.1

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 861	861	Linoleate 9S-lipoxygenase 2		PRO_0000412920
Regions
	Domain	29 – 160	132	PLAT
	Domain	163 – 861	699	Lipoxygenase
	Compositional bias	446 – 451	6	Poly-Thr
Sites
	Metal binding	522	1	Iron; catalytic By similarity
	Metal binding	527	1	Iron; catalytic By similarity
	Metal binding	713	1	Iron; catalytic By similarity
	Metal binding	717	1	Iron; catalytic By similarity
	Metal binding	861	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24379 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 25783F32C69BFA26	FASTA	861	97,067
	10 20 30 40 50 60 MIGQITSGLF GGPDDSKKLK GTVVMMNKNA LDFTDLAGSL TDKAFEFLGQ TVSFQLISSV 70 80 90 100 110 120 QGDPTNGLQG KHSNPAYLEN SLFTLTPLTA GSETAFGVTF DWNEEFGVPG AFIIKNTHIN 130 140 150 160 170 180 EFFLKSLTLE DVPNHGKVHF VCNSWVYPSF RYKSDRIFFV NQPYLPSKTP ELLRKYRENE 190 200 210 220 230 240 LLTLRGDGTG KREAWDRIYD YDIYNDLGNP DEGKENVRTT LGGSAEYPYP RRGRTGRPPT 250 260 270 280 290 300 RTDPKSESRI PLILSLDIYV PRDERFGHLK MSDFLTYALK SIVQFILPEL HALFDGTPNE 310 320 330 340 350 360 FDSFEDVLRL YEGGIKLPQG PLFKALTAAI PLEMIRELLR TDGEGILRFP TPLVIKDSKT 370 380 390 400 410 420 AWRTDEEFAR EMLAGVNPVI ISRLQEFPPK SKLDPEAYGN QNSTITAEHI EDKLDGLTVD 430 440 450 460 470 480 EAMNNNKLFI LNHHDVLIPY LRRINTTTTK TYASRTLLFL QDNGSLKPLA IELSLPHPDG 490 500 510 520 530 540 DQFGVTSKVY TPSDQGVESS IWQLAKAYVA VNDSGVHQLI SHWLNTHAVI EPFVIATNRQ 550 560 570 580 590 600 LSVLHPIHKL LYPHFRDTMN INAMARQILI NAGGVLESTV FQSKFAMEMS AVVYKDWVFP 610 620 630 640 650 660 DQALPADLVK RGVAVEDSSS PHGVRLLIED YPYAVDGLEI WSAIKSWVSD YCSFYYGSDE 670 680 690 700 710 720 EILKDNELQA WWKELREVGH GDKKNEPWWP EMERPQELID SCTTIIWIAS ALHAAVNFGQ 730 740 750 760 770 780 YPYAGYLPNR PTVSRRFMPE PGTPEYEELK KNPDKAFLKT ITAQLQTLLG VSLIEILSRH 790 800 810 820 830 840 TTDEIYLGQR ESPEWTKDKE PLAAFDKFGK KLTDIEKQII QRNGDNILTN RSGPVNAPYT 850 860 LLFPTSEGGL TGKGIPNSVS I

References

[1]

"Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns." Royo J., Vancanneyt G., Perez A.G., Sanz C., Stormann K., Rosahl S., Sanchez-Serrano J.J. J. Biol. Chem. 271:21012-21019(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY WOUNDING AND JASMONATE, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Desiree. Tissue: Tuber.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95513 mRNA. Translation: CAA64766.1.
3D structure databases
HSSP	HSSP built from PDB template 1FGT based on UniProtKB P08170.
ProteinModelPortal	O24379.
SMR	O24379. Positions 18-861.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12726.
UniPathway	UPA00382.
Family and domain databases
Gene3D	2.60.60.20. 1 hit.
InterPro	IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
PANTHER	PTHR11771. PTHR11771. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
SUPFAM	SSF49723. Lipase_LipOase. 1 hit. SSF48484. Lipoxygenase. 1 hit.
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX12_SOLTU

Accession

Primary (citable) accession number: O24379

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program