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Protein

Linoleate 13S-lipoxygenase 2-1, chloroplastic

Gene

LOX2.1

Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plant lipoxygenase involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence. May not be involved in the bulk production of jasmonate upon wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Linolenic acid is the preferred substrate, before linoleic and arachidonic acids. Has also some activity with phosphatidylglycerol, but not with galactolipids.2 Publications

Miscellaneous

99% depletion of LOX-H1 by co-suppression-mediated gene silencing has no effect on the basal or wound-induced levels of jasmonates but results in a marked reduction in the production of volatile aliphatic C6 aldehydes.

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

pH dependencei

Optimum pH is 7.0.1 Publication

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi557Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi562Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi749Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi753Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi899Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • green leaf volatile biosynthetic process Source: AgBase
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382

Names & Taxonomyi

Protein namesi
Recommended name:
Linoleate 13S-lipoxygenase 2-1, chloroplastic (EC:1.13.11.12)
Alternative name(s):
Lipoxygenase 2-1
Gene namesi
Name:LOX2.1
Synonyms:LOX-H1
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastSequence analysisAdd BLAST40
ChainiPRO_000041292741 – 899Linoleate 13S-lipoxygenase 2-1, chloroplasticAdd BLAST859

Proteomic databases

PRIDEiO24370

Expressioni

Tissue specificityi

Expressed in leaves and floral buds.1 Publication

Inductioni

Up-regulated by jasmonate and abscisic acid treatment. Up-regulated localy and systemically at the transcript levels 6 hours after wounding, but the protein levels remain constant. Not induced by pathogen infection.3 Publications

Gene expression databases

ExpressionAtlasiO24370 baseline and differential

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400081909

Structurei

3D structure databases

ProteinModelPortaliO24370
SMRiO24370
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 200PLATPROSITE-ProRule annotationAdd BLAST123
Domaini203 – 899LipoxygenasePROSITE-ProRule annotationAdd BLAST697

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1 Eukaryota
ENOG410YN4N LUCA
InParanoidiO24370
KOiK00454

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPQLQQSS QSTKALIPSW NTNPLFLASF PINILNKNFR LKKKNNFRVH
60 70 80 90 100
HNYNGASTTK AVLSSTEKAT GVKAVVTVQK QVNLNLSRGL DDIGDLLGKS
110 120 130 140 150
LLLWIVAAEL DHKTGIEKPG IRAYAHRGRD VDGDTHYEAD FVIPQDFGEV
160 170 180 190 200
GAILIENEHH KEMYVKNIVI DGFVHGKVEI TCNSWVHSKF DNPDKRIFFT
210 220 230 240 250
NKSYLPSQTP SGVSRLREEE LVTLRGDGIG ERKVFERIYD YDVYNDLGEA
260 270 280 290 300
DSNNDDAKRP VLGGKELPYP RRCKTGRPRS KKDPLSETRS TFVYVPRDEA
310 320 330 340 350
FSEVKSVAFS GNTVYSVLHA VVPALESVVT DPNLGFPHFP AIDSLFNVGV
360 370 380 390 400
DLPGLGDKKS GLFNVVPRLI KAISDTRKDV LLFESPQLVQ RDKFSWFRDV
410 420 430 440 450
EFARQTLAGL NPYSIRLVTE WPLRSKLDPK VYGPPESEIT KELIEKEIGN
460 470 480 490 500
YMTVEQAVQQ KKLFILDYHD LLLPYVNKVN ELKGSMLYGS RTIFFLTPQG
510 520 530 540 550
TLKPLAIELT RPPVDDKPQW KEVYSPNDWN ATGAWLWKLA KAHVLSHDSG
560 570 580 590 600
YHQLVSHWLR THCCTEPYII ASNRQLSAMH PIYRLLHPHF RYTMEINALA
610 620 630 640 650
REALINANGV IESSFFPGKY AIELSSIAYG AEWRFDQEAL PQNLISRGLA
660 670 680 690 700
VEDPNEPHGL KLAIEDYPFA NDGLVLWDIL KQWVTNYVNH YYPQTNLIES
710 720 730 740 750
DKELQAWWSE IKNVGHGDKR DEPWWPELKT PNDLIGIITT IVWVTSGHHA
760 770 780 790 800
AVNFGQYSYA GYFPNRPTVA RSKMPTEDPT AEEWEWFMNK PEEALLRCFP
810 820 830 840 850
SQIQATKVMA ILDVLSNHSP DEEYIGEKIE PYWAEDPVIN AAFEVFSGKL
860 870 880 890
KELEGIIDAR NNDSKLSNRN GAGVMPYELL KPYSEPGVTG KGVPYSISI
Length:899
Mass (Da):101,937
Last modified:January 1, 1998 - v1
Checksum:i43E9D512C0E808FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96405 mRNA Translation: CAA65268.1
PIRiT07062
RefSeqiNP_001274843.1, NM_001287914.1
UniGeneiStu.18313

Genome annotation databases

GeneIDi102596122
KEGGisot:102596122

Similar proteinsi

Entry informationi

Entry nameiLOX21_SOLTU
AccessioniPrimary (citable) accession number: O24370
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health