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O24370

- LOX21_SOLTU

UniProt

O24370 - LOX21_SOLTU

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Protein
Linoleate 13S-lipoxygenase 2-1, chloroplastic
Gene
LOX2.1, LOX-H1
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Plant lipoxygenase involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence. May not be involved in the bulk production of jasmonate upon wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Linolenic acid is the preferred substrate, before linoleic and arachidonic acids. Has also some activity with phosphatidylglycerol, but not with galactolipids.2 Publications

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.2 Publications
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.2 Publications

Cofactori

Binds 1 iron ion per subunit By similarity.

pH dependencei

Optimum pH is 7.0.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi557 – 5571Iron; catalytic By similarity
Metal bindingi562 – 5621Iron; catalytic By similarity
Metal bindingi749 – 7491Iron; catalytic By similarity
Metal bindingi753 – 7531Iron; catalytic By similarity
Metal bindingi899 – 8991Iron; via carboxylate; catalytic By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. linoleate 13S-lipoxygenase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. oxylipin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Linoleate 13S-lipoxygenase 2-1, chloroplastic (EC:1.13.11.12)
Alternative name(s):
Lipoxygenase 2-1
Gene namesi
Name:LOX2.1
Synonyms:LOX-H1
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

Plastidchloroplast stroma. Plastidchloroplast thylakoid
Note: Associated with the non-appressed part of the thylakoid membrane.2 Publications

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB-SubCell
  2. chloroplast thylakoid Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040Chloroplast Reviewed prediction
Add
BLAST
Chaini41 – 899859Linoleate 13S-lipoxygenase 2-1, chloroplastic
PRO_0000412927Add
BLAST

Expressioni

Tissue specificityi

Expressed in leaves and floral buds.1 Publication

Inductioni

Up-regulated by jasmonate and abscisic acid treatment. Up-regulated localy and systemically at the transcript levels 6 hours after wounding, but the protein levels remain constant. Not induced by pathogen infection.3 Publications

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO24370.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 200123PLAT
Add
BLAST
Domaini203 – 899697Lipoxygenase
Add
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.
Contains 1 PLAT domain.

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00454.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_pln.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24370-1 [UniParc]FASTAAdd to Basket

« Hide

MLKPQLQQSS QSTKALIPSW NTNPLFLASF PINILNKNFR LKKKNNFRVH    50
HNYNGASTTK AVLSSTEKAT GVKAVVTVQK QVNLNLSRGL DDIGDLLGKS 100
LLLWIVAAEL DHKTGIEKPG IRAYAHRGRD VDGDTHYEAD FVIPQDFGEV 150
GAILIENEHH KEMYVKNIVI DGFVHGKVEI TCNSWVHSKF DNPDKRIFFT 200
NKSYLPSQTP SGVSRLREEE LVTLRGDGIG ERKVFERIYD YDVYNDLGEA 250
DSNNDDAKRP VLGGKELPYP RRCKTGRPRS KKDPLSETRS TFVYVPRDEA 300
FSEVKSVAFS GNTVYSVLHA VVPALESVVT DPNLGFPHFP AIDSLFNVGV 350
DLPGLGDKKS GLFNVVPRLI KAISDTRKDV LLFESPQLVQ RDKFSWFRDV 400
EFARQTLAGL NPYSIRLVTE WPLRSKLDPK VYGPPESEIT KELIEKEIGN 450
YMTVEQAVQQ KKLFILDYHD LLLPYVNKVN ELKGSMLYGS RTIFFLTPQG 500
TLKPLAIELT RPPVDDKPQW KEVYSPNDWN ATGAWLWKLA KAHVLSHDSG 550
YHQLVSHWLR THCCTEPYII ASNRQLSAMH PIYRLLHPHF RYTMEINALA 600
REALINANGV IESSFFPGKY AIELSSIAYG AEWRFDQEAL PQNLISRGLA 650
VEDPNEPHGL KLAIEDYPFA NDGLVLWDIL KQWVTNYVNH YYPQTNLIES 700
DKELQAWWSE IKNVGHGDKR DEPWWPELKT PNDLIGIITT IVWVTSGHHA 750
AVNFGQYSYA GYFPNRPTVA RSKMPTEDPT AEEWEWFMNK PEEALLRCFP 800
SQIQATKVMA ILDVLSNHSP DEEYIGEKIE PYWAEDPVIN AAFEVFSGKL 850
KELEGIIDAR NNDSKLSNRN GAGVMPYELL KPYSEPGVTG KGVPYSISI 899
Length:899
Mass (Da):101,937
Last modified:January 1, 1998 - v1
Checksum:i43E9D512C0E808FF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96405 mRNA. Translation: CAA65268.1.
PIRiT07062.
RefSeqiNP_001274843.1. NM_001287914.1.
UniGeneiStu.18313.

Genome annotation databases

GeneIDi102596122.
KEGGisot:102596122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96405 mRNA. Translation: CAA65268.1 .
PIRi T07062.
RefSeqi NP_001274843.1. NM_001287914.1.
UniGenei Stu.18313.

3D structure databases

ProteinModelPortali O24370.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102596122.
KEGGi sot:102596122.

Phylogenomic databases

KOi K00454.

Enzyme and pathway databases

UniPathwayi UPA00382 .

Family and domain databases

Gene3Di 2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProi IPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_pln.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view ]
PANTHERi PTHR11771. PTHR11771. 1 hit.
Pfami PF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view ]
PRINTSi PR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTi SM00308. LH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns."
    Royo J., Vancanneyt G., Perez A.G., Sanz C., Stormann K., Rosahl S., Sanchez-Serrano J.J.
    J. Biol. Chem. 271:21012-21019(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION BY WOUNDING; JASMONATE AND ABSCISIC ACID, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "A leaf lipoxygenase of potato induced specifically by pathogen infection."
    Kolomiets M.V., Chen H., Gladon R.J., Braun E.J., Hannapel D.J.
    Plant Physiol. 124:1121-1130(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY PATHOGEN.
  3. "Lipoxygenase H1 gene silencing reveals a specific role in supplying fatty acid hydroperoxides for aliphatic aldehyde production."
    Leon J., Royo J., Vancanneyt G., Sanz C., Silkowski H., Griffiths G., Sanchez-Serrano J.J.
    J. Biol. Chem. 277:416-423(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY.
  4. "Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts."
    Farmaki T., Sanmartin M., Jimenez P., Paneque M., Sanz C., Vancanneyt G., Leon J., Sanchez-Serrano J.J.
    J. Exp. Bot. 58:555-568(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION BY WOUNDING.

Entry informationi

Entry nameiLOX21_SOLTU
AccessioniPrimary (citable) accession number: O24370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

99% depletion of LOX-H1 by co-suppression-mediated gene silencing has no effect on the basal or wound-induced levels of jasmonates but results in a marked reduction in the production of volatile aliphatic C6 aldehydes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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