Reviewed, UniProtKB/Swiss-Prot O24364 (BAS1_SPIOL)

Names and origin

Protein names

Recommended name:
    2-Cys peroxiredoxin BAS1, chloroplastic
    EC=1.11.1.15
Alternative name(s):
    Thiol-specific antioxidant protein

Gene names

Name:

BAS1

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Homodimer; disulfide-linked, upon oxidation By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Post-translational modification	The Cys-119-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-119 (probably Cys-SOH) rapidly reacts with Cys-240-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 65	65	Chloroplast By similarity
	Chain	66 – 265	200	2-Cys peroxiredoxin BAS1, chloroplastic		PRO_0000023786
Regions
	Domain	73 – 232	160	Thioredoxin
Sites
	Active site	119	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	119		Interchain (with C-240); in linked form By similarity
	Disulfide bond	240		Interchain (with C-119); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24364-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 11F479093C2F573B	FASTA	265	28,896
	10 20 30 40 50 60 MACVASSTTL ISSPSSRVFP AKSSLSSPSV SFLRTLSSPS ASASLRSGFA RRSSLSSTSR 70 80 90 100 110 120 RSFAVKAQAD DLPLVGNKAP DFEAEAVFDQ EFIKVKLSDY IGKKYVILFF YPLDFTFVCP 130 140 150 160 170 180 TEITAFSDRH SEFEKLNTEV LGVSVDSVFS HLAWVQTDRK SGGLGDLNYP LISDVTKSIS 190 200 210 220 230 240 KSFGVLIHDQ GIALRGLFII DKEGVIQHST INNLGIGRSV DETMRTLQAL QYTGNPDEVC 250 260 PAGWKPGEKS MKPDPKLSKE YFSAI

References

[1]

"Primary structure and expression of plant homologues of animal and fungal thioredoxin-dependent peroxide reductases and bacterial alkyl hydroperoxide reductases." Baier M., Dietz K.-J. Plant Mol. Biol. 31:553-564(1996) [PubMed: 8790288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94219 mRNA. Translation: CAA63910.1.
PIR	T09211.
3D structure databases
HSSP	HSSP built from PDB template 1QMV based on UniProtKB P32119.
SMR	O24364. Positions 75-263.
ModBase	Search...
Protein-protein interaction databases
IntAct	O24364. 1 interaction.
Protein family/group databases
PeroxiBase	4408. So2CysPrx.
Enzyme and pathway databases
BRENDA	1.11.1.15. 286.
Family and domain databases
InterPro	IPR000866. Alkyl_hydroperoxide_Rdtase. IPR019479. Peroxiredoxin_C. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF10417. 1-cysPrx_C. 1 hit. PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAS1_SPIOL

Accession

Primary (citable) accession number: O24364

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)