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O24362 (PSA3_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha type-3

EC=3.4.25.1
Alternative name(s):
20S proteasome alpha subunit G
20S proteasome subunit alpha-7
Proteasome component C8
Gene names
Name:PAG1
Synonyms:PSC8
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249Proteasome subunit alpha type-3
PRO_0000124100

Sequences

Sequence LengthMass (Da)Tools
O24362 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 8B7DB2B4D74FB3FA

FASTA24927,285
        10         20         30         40         50         60 
MSSIGTGYDL SVTTFSPDGR VFQIEYAAKA VDNSGTAVGI KCKDGIVLGV EKLIQSKMML 

        70         80         90        100        110        120 
PGSNRRIHSV HRHSGMAVAG LAADGRQIVA RAKSEATNYE SVYGEAVPVK ELADRVASYV 

       130        140        150        160        170        180 
HLCTLYWWLR PFGCGVILGG YDRDGPQLYM VEPSGISYRY FGAAIGKGKQ AAKTEIEKLK 

       190        200        210        220        230        240 
LSEMTCREGI IEVAKIIYKV HDEAKDKAFE LEMSWICDES KREHQKVPDN LLQEAKAAAT 


AALEEMDAD 

« Hide

References

[1]"Characterization of 26S proteasome alpha- and beta-type and ATPase subunits from spinach and their expression during early stages of seedling development."
Ito N., Tomizawa K., Tanaka K., Matsui M., Kendrick R.E., Sato T., Nakagawa H.
Plant Mol. Biol. 34:307-316(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78173 mRNA. Translation: BAA21651.1.
PIRT09139.

3D structure databases

ProteinModelPortalO24362.
SMRO24362. Positions 2-245.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO24362.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSA3_SPIOL
AccessionPrimary (citable) accession number: O24362
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries