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O24361 (PSB5_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-5

EC=3.4.25.1
Alternative name(s):
20S proteasome subunit E
Proteasome epsilon chain
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 5555Removed in mature form Potential
PRO_0000026607
Chain56 – 272217Proteasome subunit beta type-5
PRO_0000026608

Sites

Active site561Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
O24361 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 4D800127F7CEFC3A

FASTA27229,620
        10         20         30         40         50         60 
MKLDTSGLES TAPIFRRSDF VFDGLQMTPS FDLPNPTDFD GFQKEAVQMV KPAKGTTTLA 

        70         80         90        100        110        120 
FIFKHGVMVA ADSRASMGGY ISSQSVKKII EINPYMLGTM AGGAADCQFW HRNLGIKCRL 

       130        140        150        160        170        180 
HELANKRRIS VTGASKLLAN ILYNYRGMGL SVGTMIAGWD ETGPGLYYVD SEGGRLKGMR 

       190        200        210        220        230        240 
FSVGSGSPYA YGVLDNGYKY DMTVEEASEL ARRAIYHATY RDGASGGVVS VYHVGPDGWK 

       250        260        270 
KVTGDDVGDL HFQYYPVVPA TVEQEMVEVV GA 

« Hide

References

[1]"Characterization of 26S proteasome alpha- and beta-type and ATPase subunits from spinach and their expression during early stages of seedling development."
Ito N., Tomizawa K., Tanaka K., Matsui M., Kendrick R.E., Sato T., Nakagawa H.
Plant Mol. Biol. 34:307-316(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78172 mRNA. Translation: BAA21650.1.
PIRT09132.

3D structure databases

ProteinModelPortalO24361.
SMRO24361. Positions 56-254.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPST01.A10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSB5_SPIOL
AccessionPrimary (citable) accession number: O24361
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries