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O24357

- G6PDC_SPIOL

UniProt

O24357 - G6PDC_SPIOL

Protein

Glucose-6-phosphate 1-dehydrogenase, chloroplastic

Gene

G6PD

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. May be involved in nitrite reduction.

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Enzyme regulationi

    Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei127 – 1271NADPBy similarity
    Binding sitei230 – 2301NADP; via carbonyl oxygenBy similarity
    Binding sitei230 – 2301SubstrateBy similarity
    Binding sitei298 – 2981SubstrateBy similarity
    Binding sitei317 – 3171SubstrateBy similarity
    Active sitei322 – 3221Proton acceptorBy similarity
    Binding sitei418 – 4181SubstrateBy similarity
    Binding sitei459 – 4591SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi93 – 1008NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. pentose-phosphate shunt Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase, chloroplastic (EC:1.1.1.49)
    Short name:
    G6PD
    Gene namesi
    Name:G6PD
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 574Glucose-6-phosphate 1-dehydrogenase, chloroplasticPRO_0000010439
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi145 ↔ 153Redox modulationBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiO24357.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO24357.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni260 – 2645Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24357-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEELVSCHHL PLLCLQSSVP PNGCLTFFQD SACQRCSHSE FSNGHPLNDV    50
    SLQNDVAVNP IVAKSIDPSA DLQLLPLLES VKEEPTLSII VVGASGDLAK 100
    KKIFPALFAL FYENCLPENF TVFGFSRTEM NDEELRTMIS KTLTCRIDQR 150
    ENCGEKMDHF LQRCFYHSGQ YNSEDDFSGL DCKLKEKEAG RLQNRLFYLS 200
    IPPNIFVDVV RCVSHRASSA SGWTRVIVEK PFGRDSDSSR ELTRSFKQYL 250
    SEDQIFRIDH YLGKELVENL SVLRFSNLVF EPLWSRNYIR NVQLIFSEDF 300
    GTEGRGGYFD NYGIIRDIMQ NHLLQILALF AMETPVSLDA EDIRNEKVKV 350
    LRSMKPLKLQ DVVVGQYKGH SKGNKSYSGY TDDPTVPNNS VTPTFAAAAL 400
    FIDNARWDGV PFLMKAGKAL HTKRAEIRVQ FRHVPGNLYK KTFGTDLDKA 450
    TNELVLRVQP DEAIYLKINN KVPGLGMRLD RTDLNLCYST RYRGEIPDAY 500
    ERLLLDAIEG ERRLFIRSDK LDAAWSLFTP LLKELEEKKV APELYPYGSR 550
    GPVGAHYLAA KHNVRWGDLS GEDS 574
    Length:574
    Mass (Da):65,180
    Last modified:January 1, 1998 - v1
    Checksum:iD498DB32D25849AA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000182 mRNA. Translation: CAA03939.1.
    PIRiT09088.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000182 mRNA. Translation: CAA03939.1 .
    PIRi T09088.

    3D structure databases

    ProteinModelPortali O24357.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O24357.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fink A., Diogon T., Perroud P.F., Crespi P., Greppin H.
      Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Matador.
      Tissue: Leaf.

    Entry informationi

    Entry nameiG6PDC_SPIOL
    AccessioniPrimary (citable) accession number: O24357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3