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Protein

Glucose-6-phosphate 1-dehydrogenase, chloroplastic

Gene

G6PD

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. May be involved in nitrite reduction.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (G6PD), Glucose-6-phosphate 1-dehydrogenase (G6PD), Glucose-6-phosphate 1-dehydrogenase, chloroplastic (G6PD), Glucose-6-phosphate 1-dehydrogenase (SOVF_162250), Glucose-6-phosphate 1-dehydrogenase (SOVF_183760), Glucose-6-phosphate 1-dehydrogenase (SOVF_004890)
  2. no protein annotated in this organism
  3. 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (pgdP), 6-phosphogluconate dehydrogenase, decarboxylating (SOVF_090000), 6-phosphogluconate dehydrogenase, decarboxylating (SOVF_128490), 6-phosphogluconate dehydrogenase, decarboxylating 1 (pgdC)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei127NADPBy similarity1
Binding sitei230NADP; via carbonyl oxygenBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei298SubstrateBy similarity1
Binding sitei317SubstrateBy similarity1
Active sitei322Proton acceptorBy similarity1
Binding sitei418SubstrateBy similarity1
Binding sitei459SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi93 – 100NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, chloroplastic (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:G6PD
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000010439? – 574Glucose-6-phosphate 1-dehydrogenase, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi145 ↔ 153Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO24357.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO24357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 264Substrate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiView protein in InterPro
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiView protein in Pfam
PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiView protein in PROSITE
PS00069. G6P_DEHYDROGENASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELVSCHHL PLLCLQSSVP PNGCLTFFQD SACQRCSHSE FSNGHPLNDV
60 70 80 90 100
SLQNDVAVNP IVAKSIDPSA DLQLLPLLES VKEEPTLSII VVGASGDLAK
110 120 130 140 150
KKIFPALFAL FYENCLPENF TVFGFSRTEM NDEELRTMIS KTLTCRIDQR
160 170 180 190 200
ENCGEKMDHF LQRCFYHSGQ YNSEDDFSGL DCKLKEKEAG RLQNRLFYLS
210 220 230 240 250
IPPNIFVDVV RCVSHRASSA SGWTRVIVEK PFGRDSDSSR ELTRSFKQYL
260 270 280 290 300
SEDQIFRIDH YLGKELVENL SVLRFSNLVF EPLWSRNYIR NVQLIFSEDF
310 320 330 340 350
GTEGRGGYFD NYGIIRDIMQ NHLLQILALF AMETPVSLDA EDIRNEKVKV
360 370 380 390 400
LRSMKPLKLQ DVVVGQYKGH SKGNKSYSGY TDDPTVPNNS VTPTFAAAAL
410 420 430 440 450
FIDNARWDGV PFLMKAGKAL HTKRAEIRVQ FRHVPGNLYK KTFGTDLDKA
460 470 480 490 500
TNELVLRVQP DEAIYLKINN KVPGLGMRLD RTDLNLCYST RYRGEIPDAY
510 520 530 540 550
ERLLLDAIEG ERRLFIRSDK LDAAWSLFTP LLKELEEKKV APELYPYGSR
560 570
GPVGAHYLAA KHNVRWGDLS GEDS
Length:574
Mass (Da):65,180
Last modified:January 1, 1998 - v1
Checksum:iD498DB32D25849AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000182 mRNA. Translation: CAA03939.1.
PIRiT09088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000182 mRNA. Translation: CAA03939.1.
PIRiT09088.

3D structure databases

ProteinModelPortaliO24357.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO24357.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiView protein in InterPro
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiView protein in Pfam
PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiView protein in PROSITE
PS00069. G6P_DEHYDROGENASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG6PDC_SPIOL
AccessioniPrimary (citable) accession number: O24357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.