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O24357

- G6PDC_SPIOL

UniProt

O24357 - G6PDC_SPIOL

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Protein

Glucose-6-phosphate 1-dehydrogenase, chloroplastic

Gene

G6PD

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. May be involved in nitrite reduction.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei127 – 1271NADPBy similarity
Binding sitei230 – 2301NADP; via carbonyl oxygenBy similarity
Binding sitei230 – 2301SubstrateBy similarity
Binding sitei298 – 2981SubstrateBy similarity
Binding sitei317 – 3171SubstrateBy similarity
Active sitei322 – 3221Proton acceptorBy similarity
Binding sitei418 – 4181SubstrateBy similarity
Binding sitei459 – 4591SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 1008NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, chloroplastic (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:G6PD
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 574Glucose-6-phosphate 1-dehydrogenase, chloroplasticPRO_0000010439
Transit peptidei1 – ?ChloroplastSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi145 ↔ 153Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO24357.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO24357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni260 – 2645Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24357-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEELVSCHHL PLLCLQSSVP PNGCLTFFQD SACQRCSHSE FSNGHPLNDV
60 70 80 90 100
SLQNDVAVNP IVAKSIDPSA DLQLLPLLES VKEEPTLSII VVGASGDLAK
110 120 130 140 150
KKIFPALFAL FYENCLPENF TVFGFSRTEM NDEELRTMIS KTLTCRIDQR
160 170 180 190 200
ENCGEKMDHF LQRCFYHSGQ YNSEDDFSGL DCKLKEKEAG RLQNRLFYLS
210 220 230 240 250
IPPNIFVDVV RCVSHRASSA SGWTRVIVEK PFGRDSDSSR ELTRSFKQYL
260 270 280 290 300
SEDQIFRIDH YLGKELVENL SVLRFSNLVF EPLWSRNYIR NVQLIFSEDF
310 320 330 340 350
GTEGRGGYFD NYGIIRDIMQ NHLLQILALF AMETPVSLDA EDIRNEKVKV
360 370 380 390 400
LRSMKPLKLQ DVVVGQYKGH SKGNKSYSGY TDDPTVPNNS VTPTFAAAAL
410 420 430 440 450
FIDNARWDGV PFLMKAGKAL HTKRAEIRVQ FRHVPGNLYK KTFGTDLDKA
460 470 480 490 500
TNELVLRVQP DEAIYLKINN KVPGLGMRLD RTDLNLCYST RYRGEIPDAY
510 520 530 540 550
ERLLLDAIEG ERRLFIRSDK LDAAWSLFTP LLKELEEKKV APELYPYGSR
560 570
GPVGAHYLAA KHNVRWGDLS GEDS
Length:574
Mass (Da):65,180
Last modified:January 1, 1998 - v1
Checksum:iD498DB32D25849AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000182 mRNA. Translation: CAA03939.1.
PIRiT09088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000182 mRNA. Translation: CAA03939.1 .
PIRi T09088.

3D structure databases

ProteinModelPortali O24357.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O24357.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00408 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00966. G6PD.
InterProi IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000110. G6PD. 1 hit.
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR00871. zwf. 1 hit.
PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Fink A., Diogon T., Perroud P.F., Crespi P., Greppin H.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Matador.
    Tissue: Leaf.

Entry informationi

Entry nameiG6PDC_SPIOL
AccessioniPrimary (citable) accession number: O24357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3