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O24338

- ASNS_SANAU

UniProt

O24338 - ASNS_SANAU

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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

AND1

Organism
Sandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Could play a role in remobilization of nitrogen in flowers during senescence.

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AND1
OrganismiSandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
Taxonomic identifieri61864 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaLilialesLiliaceaeSandersonia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 525524Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056928Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO24338.
SMRiO24338. Positions 1-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 517325Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24338 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLDH HGDCYLAHQR
60 70 80 90 100
LAIIDPASGD QPLYNEDKTI IVTVNGEIYN HEELRKGLPG HTFRTGSDCE
110 120 130 140 150
VIAHLYEEHG ESFIHMLDGI FSFVLLDSRN NSFVAARDAI GVTPLYIGWG
160 170 180 190 200
LDGSVWISSE MKGLNDDCEH FKFFPPGHLY SSKEGSFKRW YNPPWFSEVI
210 220 230 240 250
PSVPFDPLAL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL VASVTARYLE
260 270 280 290 300
GTKAAELWGT QLHSFCVGLE GSPDLKAAKE VANFLGTIHH EFHFTVQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KEELHLETCH KIKALHQYDC LRANKATSAW GLEARVPFLD
410 420 430 440 450
KEFVNVAMSI DPEWKMIKPD IGRIEKWILR RAFDDEENPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKAHSA LHVTDKMMLN AAHIYPHNTP TTKEAYYYRM
510 520
IFERFFPQID SPWRSKCGLQ HSKSY
Length:525
Mass (Da):59,609
Last modified:January 23, 2007 - v3
Checksum:i5C7471022A11F722
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF005724 mRNA. Translation: AAB71532.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF005724 mRNA. Translation: AAB71532.1 .

3D structure databases

ProteinModelPortali O24338.
SMRi O24338. Positions 1-510.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of cDNA encoding asparagine synthetase from Sandersonia aurantiaca."
    Eason J.R., King G.A.
    Plant Gene Register PGR97-112
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Tepal.

Entry informationi

Entry nameiASNS_SANAU
AccessioniPrimary (citable) accession number: O24338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3