O24338 (ASNS_SANAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine synthetase [glutamine-hydrolyzing]
EC=6.3.5.4

Alternative name(s):
Glutamine-dependent asparagine synthetase

Gene names

Name:

AND1

Organism

Sandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)

Taxonomic identifier

61864 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Liliales › Liliaceae › Sandersonia

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Could play a role in remobilization of nitrogen in flowers during senescence.
Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Sequence similarities	Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 525	524	Asparagine synthetase [glutamine-hydrolyzing]		PRO_0000056928
Regions
	Domain	2 – 185	184	Glutamine amidotransferase type-2
	Domain	193 – 517	325	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24338 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5C7471022A11F722	FASTA	525	59,609
	10 20 30 40 50 60 MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLDH HGDCYLAHQR LAIIDPASGD 70 80 90 100 110 120 QPLYNEDKTI IVTVNGEIYN HEELRKGLPG HTFRTGSDCE VIAHLYEEHG ESFIHMLDGI 130 140 150 160 170 180 FSFVLLDSRN NSFVAARDAI GVTPLYIGWG LDGSVWISSE MKGLNDDCEH FKFFPPGHLY 190 200 210 220 230 240 SSKEGSFKRW YNPPWFSEVI PSVPFDPLAL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL 250 260 270 280 290 300 VASVTARYLE GTKAAELWGT QLHSFCVGLE GSPDLKAAKE VANFLGTIHH EFHFTVQDGI 310 320 330 340 350 360 DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG GYLYFHKAPN 370 380 390 400 410 420 KEELHLETCH KIKALHQYDC LRANKATSAW GLEARVPFLD KEFVNVAMSI DPEWKMIKPD 430 440 450 460 470 480 IGRIEKWILR RAFDDEENPY LPKHILYRQK EQFSDGVGYS WIDGLKAHSA LHVTDKMMLN 490 500 510 520 AAHIYPHNTP TTKEAYYYRM IFERFFPQID SPWRSKCGLQ HSKSY

References

[1]	"Nucleotide sequence of cDNA encoding asparagine synthetase from Sandersonia aurantiaca." Eason J.R., King G.A. Plant Gene Register PGR97-112 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tepal.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005724 mRNA. Translation: AAB71532.1.
3D structure databases
ProteinModelPortal	O24338.
SMR	O24338. Positions 1-510.
ModBase	Search...
Protein family/group databases
MEROPS	C44.974.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001589. Asn_synthetase_glu-h. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNS_SANAU

Accession

Primary (citable) accession number: O24338

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program