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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

AND1

Organism
Sandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Could play a role in remobilization of nitrogen in flowers during senescence.

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] (AND1)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AND1
OrganismiSandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
Taxonomic identifieri61864 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaLilialesLiliaceaeSandersonia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569282 – 525Asparagine synthetase [glutamine-hydrolyzing]Add BLAST524

Structurei

3D structure databases

ProteinModelPortaliO24338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini193 – 517Asparagine synthetaseAdd BLAST325

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLDH HGDCYLAHQR
60 70 80 90 100
LAIIDPASGD QPLYNEDKTI IVTVNGEIYN HEELRKGLPG HTFRTGSDCE
110 120 130 140 150
VIAHLYEEHG ESFIHMLDGI FSFVLLDSRN NSFVAARDAI GVTPLYIGWG
160 170 180 190 200
LDGSVWISSE MKGLNDDCEH FKFFPPGHLY SSKEGSFKRW YNPPWFSEVI
210 220 230 240 250
PSVPFDPLAL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL VASVTARYLE
260 270 280 290 300
GTKAAELWGT QLHSFCVGLE GSPDLKAAKE VANFLGTIHH EFHFTVQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KEELHLETCH KIKALHQYDC LRANKATSAW GLEARVPFLD
410 420 430 440 450
KEFVNVAMSI DPEWKMIKPD IGRIEKWILR RAFDDEENPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKAHSA LHVTDKMMLN AAHIYPHNTP TTKEAYYYRM
510 520
IFERFFPQID SPWRSKCGLQ HSKSY
Length:525
Mass (Da):59,609
Last modified:January 23, 2007 - v3
Checksum:i5C7471022A11F722
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005724 mRNA. Translation: AAB71532.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005724 mRNA. Translation: AAB71532.1.

3D structure databases

ProteinModelPortaliO24338.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_SANAU
AccessioniPrimary (citable) accession number: O24338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.