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O24338 (ASNS_SANAU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene names
Name:AND1
OrganismSandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
Taxonomic identifier61864 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaLilialesLiliaceaeSandersonia

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Could play a role in remobilization of nitrogen in flowers during senescence.

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 525524Asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056928

Regions

Domain2 – 185184Glutamine amidotransferase type-2
Domain193 – 517325Asparagine synthetase
Nucleotide binding341 – 3422ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site981Glutamine By similarity
Binding site2311ATP; via carbonyl oxygen By similarity
Binding site2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3431Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
O24338 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5C7471022A11F722

FASTA52559,609
        10         20         30         40         50         60 
MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLDH HGDCYLAHQR LAIIDPASGD 

        70         80         90        100        110        120 
QPLYNEDKTI IVTVNGEIYN HEELRKGLPG HTFRTGSDCE VIAHLYEEHG ESFIHMLDGI 

       130        140        150        160        170        180 
FSFVLLDSRN NSFVAARDAI GVTPLYIGWG LDGSVWISSE MKGLNDDCEH FKFFPPGHLY 

       190        200        210        220        230        240 
SSKEGSFKRW YNPPWFSEVI PSVPFDPLAL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL 

       250        260        270        280        290        300 
VASVTARYLE GTKAAELWGT QLHSFCVGLE GSPDLKAAKE VANFLGTIHH EFHFTVQDGI 

       310        320        330        340        350        360 
DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG GYLYFHKAPN 

       370        380        390        400        410        420 
KEELHLETCH KIKALHQYDC LRANKATSAW GLEARVPFLD KEFVNVAMSI DPEWKMIKPD 

       430        440        450        460        470        480 
IGRIEKWILR RAFDDEENPY LPKHILYRQK EQFSDGVGYS WIDGLKAHSA LHVTDKMMLN 

       490        500        510        520 
AAHIYPHNTP TTKEAYYYRM IFERFFPQID SPWRSKCGLQ HSKSY 

« Hide

References

[1]"Nucleotide sequence of cDNA encoding asparagine synthetase from Sandersonia aurantiaca."
Eason J.R., King G.A.
Plant Gene Register PGR97-112
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Tepal.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF005724 mRNA. Translation: AAB71532.1.

3D structure databases

ProteinModelPortalO24338.
SMRO24338. Positions 1-510.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNS_SANAU
AccessionPrimary (citable) accession number: O24338
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways