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O24338

- ASNS_SANAU

UniProt

O24338 - ASNS_SANAU

Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

AND1

Organism
Sandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Could play a role in remobilization of nitrogen in flowers during senescence.

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:AND1
    OrganismiSandersonia aurantiaca (Christmas-bells) (Chinese-lantern lily)
    Taxonomic identifieri61864 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaLilialesLiliaceaeSandersonia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 525524Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056928Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO24338.
    SMRiO24338. Positions 1-510.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 517325Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24338-1 [UniParc]FASTAAdd to Basket

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    MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLDH HGDCYLAHQR    50
    LAIIDPASGD QPLYNEDKTI IVTVNGEIYN HEELRKGLPG HTFRTGSDCE 100
    VIAHLYEEHG ESFIHMLDGI FSFVLLDSRN NSFVAARDAI GVTPLYIGWG 150
    LDGSVWISSE MKGLNDDCEH FKFFPPGHLY SSKEGSFKRW YNPPWFSEVI 200
    PSVPFDPLAL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL VASVTARYLE 250
    GTKAAELWGT QLHSFCVGLE GSPDLKAAKE VANFLGTIHH EFHFTVQDGI 300
    DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG 350
    GYLYFHKAPN KEELHLETCH KIKALHQYDC LRANKATSAW GLEARVPFLD 400
    KEFVNVAMSI DPEWKMIKPD IGRIEKWILR RAFDDEENPY LPKHILYRQK 450
    EQFSDGVGYS WIDGLKAHSA LHVTDKMMLN AAHIYPHNTP TTKEAYYYRM 500
    IFERFFPQID SPWRSKCGLQ HSKSY 525
    Length:525
    Mass (Da):59,609
    Last modified:January 23, 2007 - v3
    Checksum:i5C7471022A11F722
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005724 mRNA. Translation: AAB71532.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005724 mRNA. Translation: AAB71532.1 .

    3D structure databases

    ProteinModelPortali O24338.
    SMRi O24338. Positions 1-510.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of cDNA encoding asparagine synthetase from Sandersonia aurantiaca."
      Eason J.R., King G.A.
      Plant Gene Register PGR97-112
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Tepal.

    Entry informationi

    Entry nameiASNS_SANAU
    AccessioniPrimary (citable) accession number: O24338
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 73 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3