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O24319

- O24319_PHAVU

UniProt

O24319 - O24319_PHAVU

Protein
Submitted name:

Purple acid phosphatase

Gene

pap

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361Sulfate 1
    Binding sitei124 – 1241Sulfate 3
    Binding sitei124 – 1241Sulfate 8; via amide nitrogen
    Binding sitei148 – 1481Sulfate 3
    Metal bindingi162 – 1621IronImported
    Metal bindingi191 – 1911IronImported
    Metal bindingi191 – 1911ZincImported
    Metal bindingi194 – 1941IronImported
    Metal bindingi228 – 2281ZincImported
    Binding sitei290 – 2901Sulfate 10
    Metal bindingi313 – 3131Zinc; via tele nitrogenImported
    Binding sitei333 – 3331Sulfate 10
    Metal bindingi350 – 3501Zinc; via pros nitrogenImported
    Metal bindingi352 – 3521Iron; via tele nitrogenImported
    Binding sitei376 – 3761Sulfate 12; via amide nitrogen
    Binding sitei410 – 4101Sulfate 13
    Binding sitei410 – 4101Sulfate 15

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    HydrolaseImported

    Keywords - Ligandi

    IronImported, Metal-bindingImported, ZincImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Purple acid phosphataseImported (EC:3.1.3.2Imported)
    Gene namesi
    Name:papImported
    OrganismiPhaseolus vulgaris (Kidney bean) (French bean)Imported
    Taxonomic identifieri3885 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727 PotentialImportedAdd
    BLAST
    Chaini28 – 459432purple acid phosphataseImportedPRO_5000064272Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi108 – 1081N-linked (GlcNAc...)Imported
    Glycosylationi136 – 1361N-linked (GlcNAc...)Imported
    Glycosylationi170 – 1701N-linked (GlcNAc...)Imported
    Glycosylationi423 – 4231N-linked (GlcNAc...)Imported

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4DHLX-ray2.30A/B/C/D34-459[»]
    4DSYX-ray2.30A/B/C/D34-459[»]
    4DT2X-ray2.70A/B/C/D34-459[»]
    ProteinModelPortaliO24319.
    SMRiO24319. Positions 36-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni401 – 4033Sulfate 13 binding

    Keywords - Domaini

    SignalImported

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVVKGLLAL ALVLNVVVVS NGGKSSNFVR KTNKNRDMPL DSDVFRVPPG    50
    YNAPQQVHIT QGDLVGRAMI ISWVTMDEPG SSAVRYWSEK NGRKRIAKGK 100
    MSTYRFFNYS SGFIHHTTIR KLKYNTKYYY EVGLRNTTRR FSFITPPQTG 150
    LDVPYTFGLI GDLGQSFDSN TTLSHYELSP KKGQTVLFVG DLSYADRYPN 200
    HDNVRWDTWG RFTERSVAYQ PWIWTAGNHE IEFAPEINET EPFKPFSYRY 250
    HVPYEASQST SPFWYSIKRA SAHIIVLSSY SAYGRGTPQY TWLKKELRKV 300
    KRSETPWLIV LMHSPLYNSY NHHFMEGEAM RTKFEAWFVK YKVDVVFAGH 350
    VHAYERSERV SNIAYKITNG LCTPVKDQSA PVYITIGDAG NYGVIDSNMI 400
    QPQPEYSAFR EASFGHGMFD IKNRTHAHFS WNRNQDGVAV EADSVWFFNR 450
    HWYPVDDST 459
    Length:459
    Mass (Da):52,857
    Last modified:January 1, 1998 - v1
    Checksum:iCC379B7C1AE4D21A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001270 mRNA. Translation: CAA04644.1.
    PIRiT51097. S51031.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001270 mRNA. Translation: CAA04644.1 .
    PIRi T51097. S51031.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4DHL X-ray 2.30 A/B/C/D 34-459 [» ]
    4DSY X-ray 2.30 A/B/C/D 34-459 [» ]
    4DT2 X-ray 2.70 A/B/C/D 34-459 [» ]
    ProteinModelPortali O24319.
    SMRi O24319. Positions 36-459.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Heterologous expression and characterization of recombinant purple acid phosphatase from red kidney bean."
      Vogel A., Borchers T., Marcus K., Meyer H.E., Krebs B., Spener F.
      Arch. Biochem. Biophys. 401:164-172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: SeedImported.
    2. "Identification of purple acid phosphatase inhibitors by fragment-based screening: promising new leads for osteoporosis therapeutics."
      Feder D., Hussein W.M., Clayton D.J., Kan M.W., Schenk G., McGeary R.P., Guddat L.W.
      Chem. Biol. Drug Des. 80:665-674(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 34-459 IN COMPLEX WITH IRON AND ZINC, GLYCOSYLATION AT ASN-108; ASN-136; ASN-170 AND ASN-423.

    Entry informationi

    Entry nameiO24319_PHAVU
    AccessioniPrimary (citable) accession number: O24319
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 1, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3