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O24319

- O24319_PHAVU

UniProt

O24319 - O24319_PHAVU

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Protein

Purple acid phosphatase

Gene

pap

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Sulfate 1
Binding sitei124 – 1241Sulfate 3
Binding sitei124 – 1241Sulfate 8; via amide nitrogen
Binding sitei148 – 1481Sulfate 3
Metal bindingi162 – 1621IronImported
Metal bindingi191 – 1911IronImported
Metal bindingi191 – 1911ZincImported
Metal bindingi194 – 1941IronImported
Metal bindingi228 – 2281ZincImported
Binding sitei290 – 2901Sulfate 10
Metal bindingi313 – 3131Zinc; via tele nitrogenImported
Binding sitei333 – 3331Sulfate 10
Metal bindingi350 – 3501Zinc; via pros nitrogenImported
Metal bindingi352 – 3521Iron; via tele nitrogenImported
Binding sitei376 – 3761Sulfate 12; via amide nitrogen
Binding sitei410 – 4101Sulfate 13
Binding sitei410 – 4101Sulfate 15

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported

Keywords - Ligandi

IronImported, Metal-bindingImported, ZincImported

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphataseUniRule annotation (EC:3.1.3.2UniRule annotation)
Gene namesi
Name:papImported
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)Imported
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 PotentialImportedAdd
BLAST
Chaini28 – 459432purple acid phosphataseImportedPRO_5000064272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Imported
Glycosylationi136 – 1361N-linked (GlcNAc...)Imported
Glycosylationi170 – 1701N-linked (GlcNAc...)Imported
Glycosylationi423 – 4231N-linked (GlcNAc...)Imported

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DHLX-ray2.30A/B/C/D34-459[»]
4DSYX-ray2.30A/B/C/D34-459[»]
4DT2X-ray2.70A/B/C/D34-459[»]
ProteinModelPortaliO24319.
SMRiO24319. Positions 36-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni401 – 4033Sulfate 13 binding

Sequence similaritiesi

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.UniRule annotation

Keywords - Domaini

SignalUniRule annotationImported

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24319-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVVKGLLAL ALVLNVVVVS NGGKSSNFVR KTNKNRDMPL DSDVFRVPPG
60 70 80 90 100
YNAPQQVHIT QGDLVGRAMI ISWVTMDEPG SSAVRYWSEK NGRKRIAKGK
110 120 130 140 150
MSTYRFFNYS SGFIHHTTIR KLKYNTKYYY EVGLRNTTRR FSFITPPQTG
160 170 180 190 200
LDVPYTFGLI GDLGQSFDSN TTLSHYELSP KKGQTVLFVG DLSYADRYPN
210 220 230 240 250
HDNVRWDTWG RFTERSVAYQ PWIWTAGNHE IEFAPEINET EPFKPFSYRY
260 270 280 290 300
HVPYEASQST SPFWYSIKRA SAHIIVLSSY SAYGRGTPQY TWLKKELRKV
310 320 330 340 350
KRSETPWLIV LMHSPLYNSY NHHFMEGEAM RTKFEAWFVK YKVDVVFAGH
360 370 380 390 400
VHAYERSERV SNIAYKITNG LCTPVKDQSA PVYITIGDAG NYGVIDSNMI
410 420 430 440 450
QPQPEYSAFR EASFGHGMFD IKNRTHAHFS WNRNQDGVAV EADSVWFFNR

HWYPVDDST
Length:459
Mass (Da):52,857
Last modified:January 1, 1998 - v1
Checksum:iCC379B7C1AE4D21A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001270 mRNA. Translation: CAA04644.1.
PIRiT51097. S51031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001270 mRNA. Translation: CAA04644.1 .
PIRi T51097. S51031.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DHL X-ray 2.30 A/B/C/D 34-459 [» ]
4DSY X-ray 2.30 A/B/C/D 34-459 [» ]
4DT2 X-ray 2.70 A/B/C/D 34-459 [» ]
ProteinModelPortali O24319.
SMRi O24319. Positions 36-459.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Heterologous expression and characterization of recombinant purple acid phosphatase from red kidney bean."
    Vogel A., Borchers T., Marcus K., Meyer H.E., Krebs B., Spener F.
    Arch. Biochem. Biophys. 401:164-172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: SeedImported.
  2. "Identification of purple acid phosphatase inhibitors by fragment-based screening: promising new leads for osteoporosis therapeutics."
    Feder D., Hussein W.M., Clayton D.J., Kan M.W., Schenk G., McGeary R.P., Guddat L.W.
    Chem. Biol. Drug Des. 80:665-674(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 34-459 IN COMPLEX WITH IRON AND ZINC, GLYCOSYLATION AT ASN-108; ASN-136; ASN-170 AND ASN-423.

Entry informationi

Entry nameiO24319_PHAVU
AccessioniPrimary (citable) accession number: O24319
Entry historyi
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3