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O24319

- O24319_PHAVU

UniProt

O24319 - O24319_PHAVU

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Protein
Submitted name:

Purple acid phosphatase

Gene
pap
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Sulfate 1
Binding sitei124 – 1241Sulfate 3
Binding sitei124 – 1241Sulfate 8; via amide nitrogen
Binding sitei148 – 1481Sulfate 3
Metal bindingi162 – 1621IronImported
Metal bindingi191 – 1911IronImported
Metal bindingi191 – 1911ZincImported
Metal bindingi194 – 1941IronImported
Metal bindingi228 – 2281ZincImported
Binding sitei290 – 2901Sulfate 10
Metal bindingi313 – 3131Zinc; via tele nitrogenImported
Binding sitei333 – 3331Sulfate 10
Metal bindingi350 – 3501Zinc; via pros nitrogenImported
Metal bindingi352 – 3521Iron; via tele nitrogenImported
Binding sitei376 – 3761Sulfate 12; via amide nitrogen
Binding sitei410 – 4101Sulfate 13
Binding sitei410 – 4101Sulfate 15

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseImported

Keywords - Ligandi

IronImported, Metal-bindingImported, ZincImported

Names & Taxonomyi

Protein namesi
Submitted name:
Purple acid phosphataseImported (EC:3.1.3.2Imported)
Gene namesi
Name:papImported
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)Imported
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 PredictedImportedAdd
BLAST
Chaini28 – 459432purple acid phosphataseImportedPRO_5000064272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Imported
Glycosylationi136 – 1361N-linked (GlcNAc...)Imported
Glycosylationi170 – 1701N-linked (GlcNAc...)Imported
Glycosylationi423 – 4231N-linked (GlcNAc...)Imported

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DHLX-ray2.30A/B/C/D34-459[»]
4DSYX-ray2.30A/B/C/D34-459[»]
4DT2X-ray2.70A/B/C/D34-459[»]
ProteinModelPortaliO24319.
SMRiO24319. Positions 36-459.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni401 – 4033Sulfate 13 binding

Keywords - Domaini

SignalImported

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24319-1 [UniParc]FASTAAdd to Basket

« Hide

MGVVKGLLAL ALVLNVVVVS NGGKSSNFVR KTNKNRDMPL DSDVFRVPPG    50
YNAPQQVHIT QGDLVGRAMI ISWVTMDEPG SSAVRYWSEK NGRKRIAKGK 100
MSTYRFFNYS SGFIHHTTIR KLKYNTKYYY EVGLRNTTRR FSFITPPQTG 150
LDVPYTFGLI GDLGQSFDSN TTLSHYELSP KKGQTVLFVG DLSYADRYPN 200
HDNVRWDTWG RFTERSVAYQ PWIWTAGNHE IEFAPEINET EPFKPFSYRY 250
HVPYEASQST SPFWYSIKRA SAHIIVLSSY SAYGRGTPQY TWLKKELRKV 300
KRSETPWLIV LMHSPLYNSY NHHFMEGEAM RTKFEAWFVK YKVDVVFAGH 350
VHAYERSERV SNIAYKITNG LCTPVKDQSA PVYITIGDAG NYGVIDSNMI 400
QPQPEYSAFR EASFGHGMFD IKNRTHAHFS WNRNQDGVAV EADSVWFFNR 450
HWYPVDDST 459
Length:459
Mass (Da):52,857
Last modified:January 1, 1998 - v1
Checksum:iCC379B7C1AE4D21A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001270 mRNA. Translation: CAA04644.1.
PIRiT51097. S51031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001270 mRNA. Translation: CAA04644.1 .
PIRi T51097. S51031.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DHL X-ray 2.30 A/B/C/D 34-459 [» ]
4DSY X-ray 2.30 A/B/C/D 34-459 [» ]
4DT2 X-ray 2.70 A/B/C/D 34-459 [» ]
ProteinModelPortali O24319.
SMRi O24319. Positions 36-459.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Heterologous expression and characterization of recombinant purple acid phosphatase from red kidney bean."
    Vogel A., Borchers T., Marcus K., Meyer H.E., Krebs B., Spener F.
    Arch. Biochem. Biophys. 401:164-172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: SeedImported.
  2. "Identification of purple acid phosphatase inhibitors by fragment-based screening: promising new leads for osteoporosis therapeutics."
    Feder D., Hussein W.M., Clayton D.J., Kan M.W., Schenk G., McGeary R.P., Guddat L.W.
    Chem. Biol. Drug Des. 80:665-674(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 34-459 IN COMPLEX WITH IRON AND ZINC, GLYCOSYLATION AT ASN-108; ASN-136; ASN-170 AND ASN-423.

Entry informationi

Entry nameiO24319_PHAVU
AccessioniPrimary (citable) accession number: O24319
Entry historyi
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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