ID   TCMO_POPTM              Reviewed;         505 AA.
AC   O24312;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   24-MAR-2009, entry version 50.
DE   RecName: Full=Trans-cinnamate 4-monooxygenase;
DE            EC=1.14.13.11;
DE   AltName: Full=Cinnamic acid 4-hydroxylase;
DE            Short=CA4H;
DE            Short=C4H;
DE   AltName: Full=P450C4H;
DE   AltName: Full=Cytochrome P450 73;
GN   Name=CYP73A13;
OS   Populus tremuloides (Quaking aspen).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=3693;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ge L., Chiang V.L.;
RT   "A full-length cDNA encoding trans-cinnamate 4-hydroxylase from
RT   developing xylem of Populus tremuloides.";
RL   (er) Plant Gene Register PGR96-075.
CC   -!- FUNCTION: Controls carbon flux to pigments essential for
CC       pollination or UV protection, to numerous pytoalexins synthesized
CC       by plants when challenged by pathogens, and to lignins.
CC   -!- CATALYTIC ACTIVITY: Trans-cinnamate + NADPH + O(2) = 4-
CC       hydroxycinnamate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Phenylpropanoid metabolism; 4-coumaric acid biosynthesis;
CC       4-coumaric acid from trans-cinnamic acid: step 1/1.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; U47293; AAB67874.1; -; mRNA.
DR   BRENDA; 1.14.13.11; 262298.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0016710; F:trans-cinnamate 4-monooxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    505       Trans-cinnamate 4-monooxygenase.
FT                                /FTId=PRO_0000052252.
FT   METAL       447    447       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   505 AA;  58020 MW;  5BC05C4BCD1474CE CRC64;
     MDLLLLEKTL LGSFVAILVA ILVSKLRGKR FKLPPGPLPV PVFGNWLQVG DDLNHRNLTD
     LAKKFGDILL LRMGQRNLVV VSSPELSKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT
     VYGEHWRKMR RIMTVPFFTN KVVQQYRYGW EEEAAQVVED VKKNPGAATH GIVLRRRLQL
     MMYNNMYRIM FDRRFESEED PLFNKLKALN GERSRLAQSF DYNYGDFIPI LRPFLRGYLK
     ICQEVKERRL QLFKDYFVDE RKKLASTKNM CNEGLKCAID HILDAQKKGE INEDNVLYIV
     ENINVAAIET TLWSIEWGIA ELVNHPEIQK KLRHELDTLL GPGHQITEPD TYKLPYLNAV
     VKETLRLRMA IPLLVPHMNL HDAKLGGFDI PAESKILVNA WWLANNPAHW KNPEEFRPER
     FLEEEAKVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT LGRLVQNFEL LPPPGQSKID
     TSEKGGQFSL HILKHSTIVA KPRSF
//