Reviewed, UniProtKB/Swiss-Prot O24312 (TCMO_POPTM)

Names and origin

Protein names

Recommended name:
    Trans-cinnamate 4-monooxygenase
    EC=1.14.13.11
Alternative name(s):
    Cinnamic acid 4-hydroxylase
      Short name=CA4H
      Short name=C4H
    P450C4H
    Cytochrome P450 73

Gene names

Name:

CYP73A13

Organism

Populus tremuloides (Quaking aspen)

Taxonomic identifier

3693 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; 4-coumaric acid biosynthesis; 4-coumaric acid from trans-cinnamic acid: step 1/1.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052252
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24312-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5BC05C4BCD1474CE	FASTA	505	58,020
	10 20 30 40 50 60 MDLLLLEKTL LGSFVAILVA ILVSKLRGKR FKLPPGPLPV PVFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKKFGDILL LRMGQRNLVV VSSPELSKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRYGW EEEAAQVVED VKKNPGAATH GIVLRRRLQL 190 200 210 220 230 240 MMYNNMYRIM FDRRFESEED PLFNKLKALN GERSRLAQSF DYNYGDFIPI LRPFLRGYLK 250 260 270 280 290 300 ICQEVKERRL QLFKDYFVDE RKKLASTKNM CNEGLKCAID HILDAQKKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHPEIQK KLRHELDTLL GPGHQITEPD TYKLPYLNAV 370 380 390 400 410 420 VKETLRLRMA IPLLVPHMNL HDAKLGGFDI PAESKILVNA WWLANNPAHW KNPEEFRPER 430 440 450 460 470 480 FLEEEAKVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT LGRLVQNFEL LPPPGQSKID 490 500 TSEKGGQFSL HILKHSTIVA KPRSF

References

[1]	"A full-length cDNA encoding trans-cinnamate 4-hydroxylase from developing xylem of Populus tremuloides." Ge L., Chiang V.L. Plant Gene Register PGR96-075 Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47293 mRNA. Translation: AAB67874.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.11. 262298.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_POPTM

Accession

Primary (citable) accession number: O24312

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	March 24, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)