Reviewed, UniProtKB/Swiss-Prot O24296 (GPX1_PEA)

Names and origin

Protein names	Recommended name:     Phospholipid hydroperoxide glutathione peroxidase, chloroplastic       Short name=PHGPx     EC=1.11.1.12
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O.
Subcellular location	Plastid › chloroplast stroma.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: InterPro phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 64	64	Chloroplast Potential
	Chain	65 – 236	172	Phospholipid hydroperoxide glutathione peroxidase, chloroplastic		PRO_0000013087
Sites
	Active site	111	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24296-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 85300731A3455E5D	FASTA	236	26,400
	10 20 30 40 50 60 MASMAFSTTF FTPLRDFNQP RTNSTPSTSL PFTKSSIASS KSPFFQLGFS QQASSNFPIV 70 80 90 100 110 120 PSKTRSFSVN AKAIKDKTIY DFTVKDIDKK DVSLSKFKGK VLLIVNVASR CGLTSSNYTE 130 140 150 160 170 180 LSHLYENFKN KGLEVLAFPC NQFGMQEPGS NEEIKQFACT KFKAEFPIFD KVDVNGPFTA 190 200 210 220 230 PVYQFLKSSS GGFFGDIVKW NFEKFLVDKN GKVVERYPPT TSPFQIEKDI QKLLAA

References

[1]	"Identification of cDNAS encoding plastid-targeted glutathione peroxidase." Mullineaux P.M., Karpinski S., Jimenez A., Cleary S.P., Robinson C., Creissen G.P. Plant J. 13:375-379(1998) [PubMed: 9680987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Birte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ000508 mRNA. Translation: CAA04142.1.
PIR	T06462.
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
ModBase	Search...
Protein family/group databases
PeroxiBase	2897. PsGPx01.
Enzyme and pathway databases
BRENDA	1.11.1.12. 287.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX1_PEA

Accession

Primary (citable) accession number: O24296

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)