##gff-version 3
O24174	UniProtKB	Chain	1	505	.	.	.	ID=PRO_0000056530;Note=Betaine aldehyde dehydrogenase 1	
O24174	UniProtKB	Motif	503	505	.	.	.	Note=Microbody targeting signal;Ontology_term=ECO:0000255;evidence=ECO:0000255	
O24174	UniProtKB	Active site	262	262	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
O24174	UniProtKB	Active site	296	296	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
O24174	UniProtKB	Binding site	163	172	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Binding site	240	245	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
O24174	UniProtKB	Binding site	262	263	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Binding site	262	262	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Binding site	294	297	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Binding site	296	296	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Binding site	455	455	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Binding site	461	461	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
O24174	UniProtKB	Mutagenesis	164	164	.	.	.	Note=Slightly reduced affinity for NAD%2C 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald)%2C but 2-fold decrease in catalytic efficiency. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22534193;Dbxref=PMID:22534193	
O24174	UniProtKB	Mutagenesis	172	172	.	.	.	Note=Slightly reduced affinity for NAD%2C enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold). W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22534193;Dbxref=PMID:22534193	
O24174	UniProtKB	Mutagenesis	172	172	.	.	.	Note=Slightly reduced affinity for NAD%2C but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald. W->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22534193;Dbxref=PMID:22534193