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Protein

Betaine aldehyde dehydrogenase 1

Gene

BADH1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dehydrogenase that can use N-acetyl-c-aminobutyraldehyde (NAGABald), gamma-guanidinobutyraldehyde (GGBald), betaine aldehyde (Bet-ald), gamma-aminobutyraldehyde (GAB-ald), acetaldehyde, 4-aminobutylaldehyde (AB-ald), 3-aminopropionaldehyde (AP-ald), 4-N-trimethylaminobutyraldehyde (TMAB-ald) and 3-N-trimethylaminopropionaldehyde (TMAP-ald) as substrates. Catalyzes the oxidation of GAB-ald more efficiently than Bet-ald. May convert acetaldehyde into acetate, thus facilitating the production of acetyl-CoA in peroxisomes under anaerobic conditions.3 Publications

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.1 Publication

Kineticsi

  1. KM=420 µM for N-acetyl-c-aminobutyraldehyde (NAGABald, in PubMed:18704694)3 Publications
  2. KM=545 µM for gamma-guanidinobutyraldehyde (GGBald, in PubMed:18704694)3 Publications
  3. KM=3 mM for betaine aldehyde (in PubMed:18704694)3 Publications
  4. KM=497 µM for gamma-aminobutyraldehyde (in PubMed:18704694)3 Publications
  5. KM=1.29 mM for betaine aldehyde (at 30 degrees Celsius, in PubMed:22534193)3 Publications
  6. KM=432 µM for gamma-aminobutyraldehyde (at 30 degrees Celsius, in PubMed:22534193)3 Publications
  7. KM=99 µM for acetaldehyde (at 30 degrees Celsius, in PubMed:22534193)3 Publications
  8. KM=2.6 mM for betaine aldehyde (in PubMed:19850038)3 Publications
  9. KM=4.5 µM for 4-aminobutylaldehyde (AB-ald, in PubMed:19850038)3 Publications
  10. KM=17 µM for 3-aminopropionaldehyde (AP-ald, in PubMed:19850038)3 Publications
  11. KM=7.8 µM for 4-N-trimethylaminobutyraldehyde (TMAB-ald, in PubMed:19850038)3 Publications
  12. KM=35 µM for 3-N-trimethylaminopropionaldehyde (TMAP-ald, in PubMed:19850038)3 Publications
  13. KM=130 µM for acetaldehyde (in PubMed:19850038)3 Publications

Vmax=0.71 µmol/min/mg enzyme with acetaldehyde as substrate (in PubMed:19850038)3 Publications

pH dependencei

Optimum pH is 9.5.3 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei262 – 2621By similarity
Binding sitei262 – 2621Betaine aldehyde (Bet-ald)Curated
Active sitei296 – 2961By similarity
Binding sitei296 – 2961Gamma-4-aminobutyraldehyde (GAB-ald)Curated
Binding sitei455 – 4551Betaine aldehyde (Bet-ald)Curated
Binding sitei461 – 4611Gamma-4-aminobutyraldehyde (GAB-ald)Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi240 – 2456NADBy similarity

GO - Molecular functioni

  1. betaine-aldehyde dehydrogenase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to anoxia Source: UniProtKB
  2. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.8. 4460.
ReactomeiREACT_295428. Carnitine synthesis.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
Betaine aldehyde dehydrogenase 1 (EC:1.2.1.8)
Short name:
OsBADH1
Gene namesi
Name:BADH1
Ordered Locus Names:Os04g0464200, LOC_Os04g39020
ORF Names:OSJNBa0060P14.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 4

Organism-specific databases

GrameneiO24174.

Subcellular locationi

  1. Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi164 – 1641N → A: Slightly reduced affinity for NAD, 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald), but 2-fold decrease in catalytic efficiency. 1 Publication
Mutagenesisi172 – 1721W → A: Slightly reduced affinity for NAD, enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold). 1 Publication
Mutagenesisi172 – 1721W → F: Slightly reduced affinity for NAD, but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Betaine aldehyde dehydrogenase 1PRO_0000056530Add
BLAST

Proteomic databases

PRIDEiO24174.

Expressioni

Inductioni

Following submergence treatment, transient decreased levels that recovers after re-aeration.1 Publication

Gene expression databases

ExpressionAtlasiO24174. baseline.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO24174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 17210Betaine aldehyde (Bet-ald) bindingCurated
Regioni262 – 2632Gamma-4-aminobutyraldehyde (GAB-ald) bindingCurated
Regioni294 – 2974Betaine aldehyde (Bet-ald) bindingCurated

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi503 – 5053Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
InParanoidiO24174.
KOiK00130.
OMAiNRLQFYI.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSAIPRR GLFIGGGWRE PSLGRRLPVV NPATEATIGD IPAATAEDVE
60 70 80 90 100
LAVSAARDAF GRDGGRHWSR APGAVRAKYL KAIAAKIKDK KSYLALLETL
110 120 130 140 150
DSGKPLDEAA GDMEDVAACF EYYADLAEAL DGKQRAPISL PMENFESYVL
160 170 180 190 200
KEPIGVVGLI TPWNYPLLMA TWKVAPALAA GCTAVLKPSE LASLTCLELG
210 220 230 240 250
GICAEIGLPP GVLNIITGLG TEAGAPLASH PHVDKIAFTG STETGKRIMI
260 270 280 290 300
TASQMVKPVS LELGGKSPLI VFDDVDIDKA VEWAMFGCFA NAGQVCSATS
310 320 330 340 350
RLLLHEKIAK RFLDRLVAWA KSIKISDPLE EGCRLGSVVS EGQYQKIMKF
360 370 380 390 400
ISTARCEGAT ILYGGARPQH LKRGFFIEPT IITNVSTSMQ IWREEVFGPV
410 420 430 440 450
ICVKEFRTER EAVELANDTH YGLAGAVISN DLERCERISK AIQSGIVWIN
460 470 480 490 500
CSQPCFVQAP WGGNKRSGFG RELGQWGLDN YLSVKQVTKY CSDEPYGWYR

PPSKL
Length:505
Mass (Da):54,648
Last modified:January 1, 1998 - v1
Checksum:i85EFA42B059A8081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001348 Genomic DNA. Translation: BAA21098.1.
AL663017 Genomic DNA. Translation: CAD41035.1.
AK103582 mRNA. No translation available.
PIRiT03394.
RefSeqiNP_001053016.1. NM_001059551.1.
UniGeneiOs.26839.

Genome annotation databases

EnsemblPlantsiOS04T0464200-01; OS04T0464200-01; OS04G0464200.
GeneIDi4336081.
KEGGiosa:4336081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001348 Genomic DNA. Translation: BAA21098.1.
AL663017 Genomic DNA. Translation: CAD41035.1.
AK103582 mRNA. No translation available.
PIRiT03394.
RefSeqiNP_001053016.1. NM_001059551.1.
UniGeneiOs.26839.

3D structure databases

ProteinModelPortaliO24174.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO24174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS04T0464200-01; OS04T0464200-01; OS04G0464200.
GeneIDi4336081.
KEGGiosa:4336081.

Organism-specific databases

GrameneiO24174.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
InParanoidiO24174.
KOiK00130.
OMAiNRLQFYI.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.
BRENDAi1.2.1.8. 4460.
ReactomeiREACT_295428. Carnitine synthesis.

Gene expression databases

ExpressionAtlasiO24174. baseline.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes."
    Nakamura T., Yokota S., Muramoto Y., Tsutsui K., Oguri Y., Fukui K., Takabe T.
    Plant J. 11:1115-1120(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  4. "Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice."
    Bradbury L.M., Gillies S.A., Brushett D.J., Waters D.L., Henry R.J.
    Plant Mol. Biol. 68:439-449(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
  5. "OsBADH1 is possibly involved in acetaldehyde oxidation in rice plant peroxisomes."
    Mitsuya S., Yokota Y., Fujiwara T., Mori N., Takabe T.
    FEBS Lett. 583:3625-3629(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY SUBMERGENCE, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Nipponbare.
  6. "Dissecting substrate specificity of two rice BADH isoforms: Enzyme kinetics, docking and molecular dynamics simulation studies."
    Jiamsomboon K., Treesuwan W., Boonyalai N.
    Biochimie 94:1773-1783(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASN-164 AND TRP-172, BINDING OF BETAINE ALDEHYDE AND GAMMA-4-AMINOBUTYRALDEHYDE, INTERACTION WITH NAD, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiBADH1_ORYSJ
AccessioniPrimary (citable) accession number: O24174
Secondary accession number(s): Q7F9Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.