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O24174

- BADH1_ORYSJ

UniProt

O24174 - BADH1_ORYSJ

Protein

Betaine aldehyde dehydrogenase 1

Gene

BADH1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Dehydrogenase that can use N-acetyl-c-aminobutyraldehyde (NAGABald), gamma-guanidinobutyraldehyde (GGBald), betaine aldehyde (Bet-ald), gamma-aminobutyraldehyde (GAB-ald), acetaldehyde, 4-aminobutylaldehyde (AB-ald), 3-aminopropionaldehyde (AP-ald), 4-N-trimethylaminobutyraldehyde (TMAB-ald) and 3-N-trimethylaminopropionaldehyde (TMAP-ald) as substrates. Catalyzes the oxidation of GAB-ald more efficiently than Bet-ald. May convert acetaldehyde into acetate, thus facilitating the production of acetyl-CoA in peroxisomes under anaerobic conditions.3 Publications

    Catalytic activityi

    Betaine aldehyde + NAD+ + H2O = betaine + NADH.1 Publication

    Kineticsi

    1. KM=420 µM for N-acetyl-c-aminobutyraldehyde (NAGABald, in PubMed:18704694)3 Publications
    2. KM=545 µM for gamma-guanidinobutyraldehyde (GGBald, in PubMed:18704694)3 Publications
    3. KM=3 mM for betaine aldehyde (in PubMed:18704694)3 Publications
    4. KM=497 µM for gamma-aminobutyraldehyde (in PubMed:18704694)3 Publications
    5. KM=1.29 mM for betaine aldehyde (at 30 degrees Celsius, in PubMed:22534193)3 Publications
    6. KM=432 µM for gamma-aminobutyraldehyde (at 30 degrees Celsius, in PubMed:22534193)3 Publications
    7. KM=99 µM for acetaldehyde (at 30 degrees Celsius, in PubMed:22534193)3 Publications
    8. KM=2.6 mM for betaine aldehyde (in PubMed:19850038)3 Publications
    9. KM=4.5 µM for 4-aminobutylaldehyde (AB-ald, in PubMed:19850038)3 Publications
    10. KM=17 µM for 3-aminopropionaldehyde (AP-ald, in PubMed:19850038)3 Publications
    11. KM=7.8 µM for 4-N-trimethylaminobutyraldehyde (TMAB-ald, in PubMed:19850038)3 Publications
    12. KM=35 µM for 3-N-trimethylaminopropionaldehyde (TMAP-ald, in PubMed:19850038)3 Publications
    13. KM=130 µM for acetaldehyde (in PubMed:19850038)3 Publications

    Vmax=0.71 µmol/min/mg enzyme with acetaldehyde as substrate (in PubMed:19850038)3 Publications

    pH dependencei

    Optimum pH is 9.5.3 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei262 – 2621By similarity
    Binding sitei262 – 2621Betaine aldehyde (Bet-ald)Curated
    Active sitei296 – 2961By similarity
    Binding sitei296 – 2961Gamma-4-aminobutyraldehyde (GAB-ald)Curated
    Binding sitei455 – 4551Betaine aldehyde (Bet-ald)Curated
    Binding sitei461 – 4611Gamma-4-aminobutyraldehyde (GAB-ald)Curated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi240 – 2456NADBy similarity

    GO - Molecular functioni

    1. betaine-aldehyde dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to anoxia Source: UniProtKB
    2. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00529; UER00386.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Betaine aldehyde dehydrogenase 1 (EC:1.2.1.8)
    Short name:
    OsBADH1
    Gene namesi
    Name:BADH1
    Ordered Locus Names:Os04g0464200, LOC_Os04g39020
    ORF Names:OSJNBa0060P14.8
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 4

    Organism-specific databases

    GrameneiO24174.

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi164 – 1641N → A: Slightly reduced affinity for NAD, 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald), but 2-fold decrease in catalytic efficiency. 1 Publication
    Mutagenesisi172 – 1721W → A: Slightly reduced affinity for NAD, enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold). 1 Publication
    Mutagenesisi172 – 1721W → F: Slightly reduced affinity for NAD, but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 505505Betaine aldehyde dehydrogenase 1PRO_0000056530Add
    BLAST

    Proteomic databases

    PRIDEiO24174.

    Expressioni

    Inductioni

    Following submergence treatment, transient decreased levels that recovers after re-aeration.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO24174.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 17210Betaine aldehyde (Bet-ald) bindingCurated
    Regioni262 – 2632Gamma-4-aminobutyraldehyde (GAB-ald) bindingCurated
    Regioni294 – 2974Betaine aldehyde (Bet-ald) bindingCurated

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi503 – 5053Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000271505.
    KOiK00130.
    OMAiNEICHEA.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O24174-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPSAIPRR GLFIGGGWRE PSLGRRLPVV NPATEATIGD IPAATAEDVE    50
    LAVSAARDAF GRDGGRHWSR APGAVRAKYL KAIAAKIKDK KSYLALLETL 100
    DSGKPLDEAA GDMEDVAACF EYYADLAEAL DGKQRAPISL PMENFESYVL 150
    KEPIGVVGLI TPWNYPLLMA TWKVAPALAA GCTAVLKPSE LASLTCLELG 200
    GICAEIGLPP GVLNIITGLG TEAGAPLASH PHVDKIAFTG STETGKRIMI 250
    TASQMVKPVS LELGGKSPLI VFDDVDIDKA VEWAMFGCFA NAGQVCSATS 300
    RLLLHEKIAK RFLDRLVAWA KSIKISDPLE EGCRLGSVVS EGQYQKIMKF 350
    ISTARCEGAT ILYGGARPQH LKRGFFIEPT IITNVSTSMQ IWREEVFGPV 400
    ICVKEFRTER EAVELANDTH YGLAGAVISN DLERCERISK AIQSGIVWIN 450
    CSQPCFVQAP WGGNKRSGFG RELGQWGLDN YLSVKQVTKY CSDEPYGWYR 500
    PPSKL 505
    Length:505
    Mass (Da):54,648
    Last modified:January 1, 1998 - v1
    Checksum:i85EFA42B059A8081
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001348 Genomic DNA. Translation: BAA21098.1.
    AL663017 Genomic DNA. Translation: CAD41035.1.
    AK103582 mRNA. No translation available.
    PIRiT03394.
    RefSeqiNP_001053016.1. NM_001059551.1.
    UniGeneiOs.26839.

    Genome annotation databases

    EnsemblPlantsiOS04T0464200-01; OS04T0464200-01; OS04G0464200.
    GeneIDi4336081.
    KEGGiosa:4336081.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001348 Genomic DNA. Translation: BAA21098.1 .
    AL663017 Genomic DNA. Translation: CAD41035.1 .
    AK103582 mRNA. No translation available.
    PIRi T03394.
    RefSeqi NP_001053016.1. NM_001059551.1.
    UniGenei Os.26839.

    3D structure databases

    ProteinModelPortali O24174.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O24174.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS04T0464200-01 ; OS04T0464200-01 ; OS04G0464200 .
    GeneIDi 4336081.
    KEGGi osa:4336081.

    Organism-specific databases

    Gramenei O24174.

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000271505.
    KOi K00130.
    OMAi NEICHEA.

    Enzyme and pathway databases

    UniPathwayi UPA00529 ; UER00386 .

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes."
      Nakamura T., Yokota S., Muramoto Y., Tsutsui K., Oguri Y., Fukui K., Takabe T.
      Plant J. 11:1115-1120(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    4. "Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice."
      Bradbury L.M., Gillies S.A., Brushett D.J., Waters D.L., Henry R.J.
      Plant Mol. Biol. 68:439-449(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
    5. "OsBADH1 is possibly involved in acetaldehyde oxidation in rice plant peroxisomes."
      Mitsuya S., Yokota Y., Fujiwara T., Mori N., Takabe T.
      FEBS Lett. 583:3625-3629(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY SUBMERGENCE, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Nipponbare.
    6. "Dissecting substrate specificity of two rice BADH isoforms: Enzyme kinetics, docking and molecular dynamics simulation studies."
      Jiamsomboon K., Treesuwan W., Boonyalai N.
      Biochimie 94:1773-1783(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASN-164 AND TRP-172, BINDING OF BETAINE ALDEHYDE AND GAMMA-4-AMINOBUTYRALDEHYDE, INTERACTION WITH NAD, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiBADH1_ORYSJ
    AccessioniPrimary (citable) accession number: O24174
    Secondary accession number(s): Q7F9Q3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3