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O24174

- BADH1_ORYSJ

UniProt

O24174 - BADH1_ORYSJ

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Protein

Betaine aldehyde dehydrogenase 1

Gene
BADH1, Os04g0464200, LOC_Os04g39020, OSJNBa0060P14.8
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Dehydrogenase that can use N-acetyl-c-aminobutyraldehyde (NAGABald), gamma-guanidinobutyraldehyde (GGBald), betaine aldehyde (Bet-ald), gamma-aminobutyraldehyde (GAB-ald), acetaldehyde, 4-aminobutylaldehyde (AB-ald), 3-aminopropionaldehyde (AP-ald), 4-N-trimethylaminobutyraldehyde (TMAB-ald) and 3-N-trimethylaminopropionaldehyde (TMAP-ald) as substrates. Catalyzes the oxidation of GAB-ald more efficiently than Bet-ald. May convert acetaldehyde into acetate, thus facilitating the production of acetyl-CoA in peroxisomes under anaerobic conditions.

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.

Kineticsi

  1. KM=420 µM for N-acetyl-c-aminobutyraldehyde (NAGABald, in 1 Publication)
  2. KM=545 µM for gamma-guanidinobutyraldehyde (GGBald, in 1 Publication)
  3. KM=3 mM for betaine aldehyde (in 1 Publication)
  4. KM=497 µM for gamma-aminobutyraldehyde (in 1 Publication)
  5. KM=1.29 mM for betaine aldehyde (at 30 degrees Celsius, in 1 Publication)
  6. KM=432 µM for gamma-aminobutyraldehyde (at 30 degrees Celsius, in 1 Publication)
  7. KM=99 µM for acetaldehyde (at 30 degrees Celsius, in 1 Publication)
  8. KM=2.6 mM for betaine aldehyde (in 1 Publication)
  9. KM=4.5 µM for 4-aminobutylaldehyde (AB-ald, in 1 Publication)
  10. KM=17 µM for 3-aminopropionaldehyde (AP-ald, in 1 Publication)
  11. KM=7.8 µM for 4-N-trimethylaminobutyraldehyde (TMAB-ald, in 1 Publication)
  12. KM=35 µM for 3-N-trimethylaminopropionaldehyde (TMAP-ald, in 1 Publication)
  13. KM=130 µM for acetaldehyde (in 1 Publication)

Vmax=0.71 µmol/min/mg enzyme with acetaldehyde as substrate (in 1 Publication

)

pH dependencei

Optimum pH is 9.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei262 – 2621 By similarity
Binding sitei262 – 2621Betaine aldehyde (Bet-ald) Inferred
Active sitei296 – 2961 By similarity
Binding sitei296 – 2961Gamma-4-aminobutyraldehyde (GAB-ald) Inferred
Binding sitei455 – 4551Betaine aldehyde (Bet-ald) Inferred
Binding sitei461 – 4611Gamma-4-aminobutyraldehyde (GAB-ald) Inferred

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi240 – 2456NAD By similarity

GO - Molecular functioni

  1. betaine-aldehyde dehydrogenase activity Source: UniProtKB

GO - Biological processi

  1. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
Betaine aldehyde dehydrogenase 1 (EC:1.2.1.8)
Short name:
OsBADH1
Gene namesi
Name:BADH1
Ordered Locus Names:Os04g0464200, LOC_Os04g39020
ORF Names:OSJNBa0060P14.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 4

Organism-specific databases

GrameneiO24174.

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi164 – 1641N → A: Slightly reduced affinity for NAD, 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald), but 2-fold decrease in catalytic efficiency.
Mutagenesisi172 – 1721W → A: Slightly reduced affinity for NAD, enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold).
Mutagenesisi172 – 1721W → F: Slightly reduced affinity for NAD, but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Betaine aldehyde dehydrogenase 1PRO_0000056530Add
BLAST

Proteomic databases

PRIDEiO24174.

Expressioni

Inductioni

Following submergence treatment, transient decreased levels that recovers after re-aeration.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO24174.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 17210Betaine aldehyde (Bet-ald) binding Inferred
Regioni262 – 2632Gamma-4-aminobutyraldehyde (GAB-ald) binding Inferred
Regioni294 – 2974Betaine aldehyde (Bet-ald) binding Inferred

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi503 – 5053Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiNEICHEA.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24174-1 [UniParc]FASTAAdd to Basket

« Hide

MAAPSAIPRR GLFIGGGWRE PSLGRRLPVV NPATEATIGD IPAATAEDVE    50
LAVSAARDAF GRDGGRHWSR APGAVRAKYL KAIAAKIKDK KSYLALLETL 100
DSGKPLDEAA GDMEDVAACF EYYADLAEAL DGKQRAPISL PMENFESYVL 150
KEPIGVVGLI TPWNYPLLMA TWKVAPALAA GCTAVLKPSE LASLTCLELG 200
GICAEIGLPP GVLNIITGLG TEAGAPLASH PHVDKIAFTG STETGKRIMI 250
TASQMVKPVS LELGGKSPLI VFDDVDIDKA VEWAMFGCFA NAGQVCSATS 300
RLLLHEKIAK RFLDRLVAWA KSIKISDPLE EGCRLGSVVS EGQYQKIMKF 350
ISTARCEGAT ILYGGARPQH LKRGFFIEPT IITNVSTSMQ IWREEVFGPV 400
ICVKEFRTER EAVELANDTH YGLAGAVISN DLERCERISK AIQSGIVWIN 450
CSQPCFVQAP WGGNKRSGFG RELGQWGLDN YLSVKQVTKY CSDEPYGWYR 500
PPSKL 505
Length:505
Mass (Da):54,648
Last modified:January 1, 1998 - v1
Checksum:i85EFA42B059A8081
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001348 Genomic DNA. Translation: BAA21098.1.
AL663017 Genomic DNA. Translation: CAD41035.1.
AK103582 mRNA. No translation available.
PIRiT03394.
RefSeqiNP_001053016.1. NM_001059551.1.
UniGeneiOs.26839.

Genome annotation databases

EnsemblPlantsiOS04T0464200-01; OS04T0464200-01; OS04G0464200.
GeneIDi4336081.
KEGGiosa:4336081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001348 Genomic DNA. Translation: BAA21098.1 .
AL663017 Genomic DNA. Translation: CAD41035.1 .
AK103582 mRNA. No translation available.
PIRi T03394.
RefSeqi NP_001053016.1. NM_001059551.1.
UniGenei Os.26839.

3D structure databases

ProteinModelPortali O24174.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O24174.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS04T0464200-01 ; OS04T0464200-01 ; OS04G0464200 .
GeneIDi 4336081.
KEGGi osa:4336081.

Organism-specific databases

Gramenei O24174.

Phylogenomic databases

eggNOGi COG1012.
HOGENOMi HOG000271505.
KOi K00130.
OMAi NEICHEA.

Enzyme and pathway databases

UniPathwayi UPA00529 ; UER00386 .

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes."
    Nakamura T., Yokota S., Muramoto Y., Tsutsui K., Oguri Y., Fukui K., Takabe T.
    Plant J. 11:1115-1120(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  4. "Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice."
    Bradbury L.M., Gillies S.A., Brushett D.J., Waters D.L., Henry R.J.
    Plant Mol. Biol. 68:439-449(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY.
  5. "OsBADH1 is possibly involved in acetaldehyde oxidation in rice plant peroxisomes."
    Mitsuya S., Yokota Y., Fujiwara T., Mori N., Takabe T.
    FEBS Lett. 583:3625-3629(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY SUBMERGENCE, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Nipponbare.
  6. "Dissecting substrate specificity of two rice BADH isoforms: Enzyme kinetics, docking and molecular dynamics simulation studies."
    Jiamsomboon K., Treesuwan W., Boonyalai N.
    Biochimie 94:1773-1783(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASN-164 AND TRP-172, BINDING OF BETAINE ALDEHYDE AND GAMMA-4-AMINOBUTYRALDEHYDE, INTERACTION WITH NAD, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiBADH1_ORYSJ
AccessioniPrimary (citable) accession number: O24174
Secondary accession number(s): Q7F9Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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