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Protein

Betaine aldehyde dehydrogenase 1

Gene

BADH1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dehydrogenase that can use N-acetyl-gamma-aminobutyraldehyde (NAGABald), gamma-guanidinobutyraldehyde (GGBald), betaine aldehyde (Bet-ald), gamma-aminobutyraldehyde (GAB-ald), acetaldehyde, 4-aminobutylaldehyde (AB-ald), 3-aminopropionaldehyde (AP-ald), 4-N-trimethylaminobutyraldehyde (TMAB-ald) and 3-N-trimethylaminopropionaldehyde (TMAP-ald) as substrates. Catalyzes the oxidation of GAB-ald more efficiently than Bet-ald. May convert acetaldehyde into acetate, thus facilitating the production of acetyl-CoA in peroxisomes under anaerobic conditions.3 Publications

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.1 Publication

Kineticsi

  1. KM=420 µM for N-acetyl-gamma-aminobutyraldehyde (NAGABald)1 Publication
  2. KM=545 µM for gamma-guanidinobutyraldehyde (GGBald)1 Publication
  3. KM=3 mM for betaine aldehyde1 Publication
  4. KM=497 µM for gamma-aminobutyraldehyde1 Publication
  5. KM=1.29 mM for betaine aldehyde (at 30 degrees Celsius)1 Publication
  6. KM=432 µM for gamma-aminobutyraldehyde (at 30 degrees Celsius)1 Publication
  7. KM=99 µM for acetaldehyde (at 30 degrees Celsius)1 Publication
  8. KM=2.6 mM for betaine aldehyde1 Publication
  9. KM=4.5 µM for 4-aminobutylaldehyde (AB-ald)1 Publication
  10. KM=17 µM for 3-aminopropionaldehyde (AP-ald)1 Publication
  11. KM=7.8 µM for 4-N-trimethylaminobutyraldehyde (TMAB-ald)1 Publication
  12. KM=35 µM for 3-N-trimethylaminopropionaldehyde (TMAP-ald)1 Publication
  13. KM=130 µM for acetaldehyde1 Publication
  1. Vmax=0.71 µmol/min/mg enzyme with acetaldehyde as substrate1 Publication

pH dependencei

Optimum pH is 9.5.3 Publications

Pathwayi: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine from betaine aldehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Betaine aldehyde dehydrogenase 2 (BADH2), Betaine aldehyde dehydrogenase 1 (BADH1)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from betaine aldehyde, the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei262By similarity1
Binding sitei262Betaine aldehyde (Bet-ald)Curated1
Active sitei296By similarity1
Binding sitei296Gamma-4-aminobutyraldehyde (GAB-ald)Curated1
Binding sitei455Betaine aldehyde (Bet-ald)Curated1
Binding sitei461Gamma-4-aminobutyraldehyde (GAB-ald)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi240 – 245NADBy similarity6

GO - Molecular functioni

  • betaine-aldehyde dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • cellular response to anoxia Source: UniProtKB
  • glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BRENDAi1.2.1.8 4460
ReactomeiR-OSA-71262 Carnitine synthesis
UniPathwayiUPA00529; UER00386

Names & Taxonomyi

Protein namesi
Recommended name:
Betaine aldehyde dehydrogenase 1 (EC:1.2.1.8)
Short name:
OsBADH1
Gene namesi
Name:BADH1
Ordered Locus Names:Os04g0464200, LOC_Os04g39020
ORF Names:OSJNBa0060P14.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164N → A: Slightly reduced affinity for NAD, 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald), but 2-fold decrease in catalytic efficiency. 1 Publication1
Mutagenesisi172W → A: Slightly reduced affinity for NAD, enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold). 1 Publication1
Mutagenesisi172W → F: Slightly reduced affinity for NAD, but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000565301 – 505Betaine aldehyde dehydrogenase 1Add BLAST505

Proteomic databases

PaxDbiO24174
PRIDEiO24174

Expressioni

Inductioni

Following submergence treatment, transient decreased levels that recovers after re-aeration.1 Publication

Gene expression databases

ExpressionAtlasiO24174 differential
GenevisibleiO24174 OS

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os04g39020.1

Structurei

3D structure databases

ProteinModelPortaliO24174
SMRiO24174
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 172Betaine aldehyde (Bet-ald) bindingCurated10
Regioni262 – 263Gamma-4-aminobutyraldehyde (GAB-ald) bindingCurated2
Regioni294 – 297Betaine aldehyde (Bet-ald) bindingCurated4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi503 – 505Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG2450 Eukaryota
COG1012 LUCA
HOGENOMiHOG000271505
InParanoidiO24174
KOiK00130
OMAiWNFPLDM
OrthoDBiEOG093606WE

Family and domain databases

Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

O24174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSAIPRR GLFIGGGWRE PSLGRRLPVV NPATEATIGD IPAATAEDVE
60 70 80 90 100
LAVSAARDAF GRDGGRHWSR APGAVRAKYL KAIAAKIKDK KSYLALLETL
110 120 130 140 150
DSGKPLDEAA GDMEDVAACF EYYADLAEAL DGKQRAPISL PMENFESYVL
160 170 180 190 200
KEPIGVVGLI TPWNYPLLMA TWKVAPALAA GCTAVLKPSE LASLTCLELG
210 220 230 240 250
GICAEIGLPP GVLNIITGLG TEAGAPLASH PHVDKIAFTG STETGKRIMI
260 270 280 290 300
TASQMVKPVS LELGGKSPLI VFDDVDIDKA VEWAMFGCFA NAGQVCSATS
310 320 330 340 350
RLLLHEKIAK RFLDRLVAWA KSIKISDPLE EGCRLGSVVS EGQYQKIMKF
360 370 380 390 400
ISTARCEGAT ILYGGARPQH LKRGFFIEPT IITNVSTSMQ IWREEVFGPV
410 420 430 440 450
ICVKEFRTER EAVELANDTH YGLAGAVISN DLERCERISK AIQSGIVWIN
460 470 480 490 500
CSQPCFVQAP WGGNKRSGFG RELGQWGLDN YLSVKQVTKY CSDEPYGWYR

PPSKL
Length:505
Mass (Da):54,648
Last modified:January 1, 1998 - v1
Checksum:i85EFA42B059A8081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001348 Genomic DNA Translation: BAA21098.1
AL663017 Genomic DNA Translation: CAD41035.1
AP008210 Genomic DNA Translation: BAF14930.1
AP014960 Genomic DNA Translation: BAS89584.1
AK103582 mRNA Translation: BAG96152.1
PIRiT03394
RefSeqiXP_015637091.1, XM_015781605.1
UniGeneiOs.26839

Genome annotation databases

EnsemblPlantsiOs04t0464200-01; Os04t0464200-01; Os04g0464200
GeneIDi4336081
GrameneiOs04t0464200-01; Os04t0464200-01; Os04g0464200
KEGGiosa:4336081

Similar proteinsi

Entry informationi

Entry nameiBADH1_ORYSJ
AccessioniPrimary (citable) accession number: O24174
Secondary accession number(s): Q0JCK7, Q7F9Q3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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