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O24164

- PPOM_TOBAC

UniProt

O24164 - PPOM_TOBAC

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Protein
Protoporphyrinogen oxidase, mitochondrial
Gene
PPXII, PPOX2
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

Binds 1 FAD per subunit.1 Publication

Enzyme regulationi

Inhibited by the herbicide acifluorfen.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511FAD
Binding sitei264 – 2641FAD; via amide nitrogen and carbonyl oxygen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 256FAD
Nucleotide bindingi43 – 442FAD
Nucleotide bindingi65 – 684FAD
Nucleotide bindingi473 – 4753FAD

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11759.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase, mitochondrial (EC:1.3.3.4)
Alternative name(s):
PX-2
Protoporphyrinogen IX oxidase isozyme II
Short name:
PPO II
Short name:
PPX II
Gene namesi
Name:PPXII
Synonyms:PPOX2
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Protoporphyrinogen oxidase, mitochondrial
PRO_0000135273Add
BLAST

Expressioni

Developmental stagei

Expressed in expanding premature leaves. Decreased expression in oldest leaves. Also detected in roots.1 Publication

Inductioni

Oscillating expression during diurnal growth. Maximal expression in the dark period.1 Publication

Interactioni

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195
Helixi23 – 3311
Beta strandi38 – 425
Beta strandi44 – 485
Beta strandi54 – 574
Beta strandi60 – 656
Helixi74 – 829
Helixi86 – 883
Beta strandi98 – 10811
Helixi113 – 1186
Beta strandi119 – 1224
Helixi124 – 1318
Helixi133 – 1364
Helixi152 – 1609
Helixi162 – 1665
Helixi169 – 1779
Helixi181 – 1833
Helixi186 – 1894
Helixi191 – 1999
Helixi203 – 2108
Beta strandi236 – 2383
Helixi243 – 2508
Turni255 – 2573
Beta strandi264 – 2707
Beta strandi272 – 2776
Beta strandi279 – 2857
Beta strandi287 – 2904
Beta strandi296 – 3027
Helixi306 – 3105
Beta strandi312 – 3209
Beta strandi331 – 34212
Helixi343 – 3453
Beta strandi346 – 3483
Beta strandi352 – 3565
Helixi359 – 3646
Beta strandi368 – 3736
Helixi374 – 3774
Helixi379 – 3813
Beta strandi386 – 39510
Helixi399 – 4013
Helixi406 – 42116
Beta strandi428 – 44013
Helixi446 – 45914
Beta strandi463 – 4653
Beta strandi468 – 4714
Helixi475 – 49319

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SEZX-ray2.90A/B1-504[»]
ProteinModelPortaliO24164.
SMRiO24164. Positions 13-497.

Miscellaneous databases

EvolutionaryTraceiO24164.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

O24164-1 [UniParc]FASTAAdd to Basket

« Hide

MAPSAGEDKH SSAKRVAVIG AGVSGLAAAY KLKIHGLNVT VFEAEGKAGG    50
KLRSVSQDGL IWDEGANTMT ESEGDVTFLI DSLGLREKQQ FPLSQNKRYI 100
ARNGTPVLLP SNPIDLIKSN FLSTGSKLQM LLEPILWKNK KLSQVSDSHE 150
SVSGFFQRHF GKEVVDYLID PFVAGTCGGD PDSLSMHHSF PELWNLEKRF 200
GSVILGAIRS KLSPKNEKKQ GPPKTSANKK RQRGSFSFLG GMQTLTDAIC 250
KDLREDELRL NSRVLELSCS CTEDSAIDSW SIISASPHKR QSEEESFDAV 300
IMTAPLCDVK SMKIAKRGNP FLLNFIPEVD YVPLSVVITT FKRENVKYPL 350
EGFGVLVPSK EQQHGLKTLG TLFSSMMFPD RAPNNVYLYT TFVGGSRNRE 400
LAKASRTELK EIVTSDLKQL LGAEGEPTYV NHLYWSKAFP LYGHNYDSVL 450
DAIDKMEKNL PGLFYAGNHR GGLSVGKALS SGCNAADLVI SYLESVSTDS 500
KRHC 504
Length:504
Mass (Da):55,407
Last modified:January 1, 1998 - v1
Checksum:iB85B55EC881DC00A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13466 mRNA. Translation: CAA73866.1.
AB020500 mRNA. Translation: BAA34712.1.
AF044129 mRNA. Translation: AAD02291.1.
PIRiT04076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13466 mRNA. Translation: CAA73866.1 .
AB020500 mRNA. Translation: BAA34712.1 .
AF044129 mRNA. Translation: AAD02291.1 .
PIRi T04076.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SEZ X-ray 2.90 A/B 1-504 [» ]
ProteinModelPortali O24164.
SMRi O24164. Positions 13-497.
ModBasei Search...

Chemistry

BindingDBi O24164.
ChEMBLi CHEMBL1926489.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
BioCyci MetaCyc:MONOMER-11759.

Miscellaneous databases

EvolutionaryTracei O24164.

Family and domain databases

Gene3Di 3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase."
    Lermontova I., Kruse E., Mock H.-P., Grimm B.
    Proc. Natl. Acad. Sci. U.S.A. 94:8895-8900(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, SUBCELLULAR LOCATION.
    Strain: cv. SR1.
  2. "Molecular characterization of photomixotrophic cultured tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides."
    Watanabe N., Che F., Iwano M., Takayama S., Nakano T., Yoshida S., Isogai A.
    Plant Physiol. 118:751-758(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Samsun NN.
  3. "The molecular basis of photobleaching herbicide resistance in Tobacco."
    Horikoshi M., Mametsuka K., Hirooka T.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. SR1.
  4. "Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis."
    Koch M., Breithaupt C., Kiefersauer R., Freigang J., Huber R., Messerschmidt A.
    EMBO J. 23:1720-1728(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) IN COMPLEX WITH SYNTHETIC INHIBITOR AND FAD, IDENTIFICATION BY MASS SPECTROMETRY, COFACTOR.

Entry informationi

Entry nameiPPOM_TOBAC
AccessioniPrimary (citable) accession number: O24164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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