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O24164

- PPOM_TOBAC

UniProt

O24164 - PPOM_TOBAC

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Protein

Protoporphyrinogen oxidase, mitochondrial

Gene

PPXII

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Enzyme regulationi

Inhibited by the herbicide acifluorfen.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511FAD1 Publication
Binding sitei264 – 2641FAD; via amide nitrogen and carbonyl oxygen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 256FAD1 Publication
Nucleotide bindingi43 – 442FAD1 Publication
Nucleotide bindingi65 – 684FAD1 Publication
Nucleotide bindingi473 – 4753FAD1 Publication

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11759.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase, mitochondrial (EC:1.3.3.4)
Alternative name(s):
PX-2
Protoporphyrinogen IX oxidase isozyme II
Short name:
PPO II
Short name:
PPX II
Gene namesi
Name:PPXII
Synonyms:PPOX2
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Protoporphyrinogen oxidase, mitochondrialPRO_0000135273Add
BLAST

Expressioni

Developmental stagei

Expressed in expanding premature leaves. Decreased expression in oldest leaves. Also detected in roots.1 Publication

Inductioni

Oscillating expression during diurnal growth. Maximal expression in the dark period.1 Publication

Interactioni

Structurei

Secondary structure

1
504
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Helixi23 – 3311Combined sources
Beta strandi38 – 425Combined sources
Beta strandi44 – 485Combined sources
Beta strandi54 – 574Combined sources
Beta strandi60 – 656Combined sources
Helixi74 – 829Combined sources
Helixi86 – 883Combined sources
Beta strandi98 – 10811Combined sources
Helixi113 – 1186Combined sources
Beta strandi119 – 1224Combined sources
Helixi124 – 1318Combined sources
Helixi133 – 1364Combined sources
Helixi152 – 1609Combined sources
Helixi162 – 1665Combined sources
Helixi169 – 1779Combined sources
Helixi181 – 1833Combined sources
Helixi186 – 1894Combined sources
Helixi191 – 1999Combined sources
Helixi203 – 2108Combined sources
Beta strandi236 – 2383Combined sources
Helixi243 – 2508Combined sources
Turni255 – 2573Combined sources
Beta strandi264 – 2707Combined sources
Beta strandi272 – 2776Combined sources
Beta strandi279 – 2857Combined sources
Beta strandi287 – 2904Combined sources
Beta strandi296 – 3027Combined sources
Helixi306 – 3105Combined sources
Beta strandi312 – 3209Combined sources
Beta strandi331 – 34212Combined sources
Helixi343 – 3453Combined sources
Beta strandi346 – 3483Combined sources
Beta strandi352 – 3565Combined sources
Helixi359 – 3646Combined sources
Beta strandi368 – 3736Combined sources
Helixi374 – 3774Combined sources
Helixi379 – 3813Combined sources
Beta strandi386 – 39510Combined sources
Helixi399 – 4013Combined sources
Helixi406 – 42116Combined sources
Beta strandi428 – 44013Combined sources
Helixi446 – 45914Combined sources
Beta strandi463 – 4653Combined sources
Beta strandi468 – 4714Combined sources
Helixi475 – 49319Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SEZX-ray2.90A/B1-504[»]
ProteinModelPortaliO24164.
SMRiO24164. Positions 13-497.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24164.

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

O24164-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSAGEDKH SSAKRVAVIG AGVSGLAAAY KLKIHGLNVT VFEAEGKAGG
60 70 80 90 100
KLRSVSQDGL IWDEGANTMT ESEGDVTFLI DSLGLREKQQ FPLSQNKRYI
110 120 130 140 150
ARNGTPVLLP SNPIDLIKSN FLSTGSKLQM LLEPILWKNK KLSQVSDSHE
160 170 180 190 200
SVSGFFQRHF GKEVVDYLID PFVAGTCGGD PDSLSMHHSF PELWNLEKRF
210 220 230 240 250
GSVILGAIRS KLSPKNEKKQ GPPKTSANKK RQRGSFSFLG GMQTLTDAIC
260 270 280 290 300
KDLREDELRL NSRVLELSCS CTEDSAIDSW SIISASPHKR QSEEESFDAV
310 320 330 340 350
IMTAPLCDVK SMKIAKRGNP FLLNFIPEVD YVPLSVVITT FKRENVKYPL
360 370 380 390 400
EGFGVLVPSK EQQHGLKTLG TLFSSMMFPD RAPNNVYLYT TFVGGSRNRE
410 420 430 440 450
LAKASRTELK EIVTSDLKQL LGAEGEPTYV NHLYWSKAFP LYGHNYDSVL
460 470 480 490 500
DAIDKMEKNL PGLFYAGNHR GGLSVGKALS SGCNAADLVI SYLESVSTDS

KRHC
Length:504
Mass (Da):55,407
Last modified:January 1, 1998 - v1
Checksum:iB85B55EC881DC00A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13466 mRNA. Translation: CAA73866.1.
AB020500 mRNA. Translation: BAA34712.1.
AF044129 mRNA. Translation: AAD02291.1.
PIRiT04076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13466 mRNA. Translation: CAA73866.1 .
AB020500 mRNA. Translation: BAA34712.1 .
AF044129 mRNA. Translation: AAD02291.1 .
PIRi T04076.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SEZ X-ray 2.90 A/B 1-504 [» ]
ProteinModelPortali O24164.
SMRi O24164. Positions 13-497.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi O24164.
ChEMBLi CHEMBL1926489.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
BioCyci MetaCyc:MONOMER-11759.

Miscellaneous databases

EvolutionaryTracei O24164.

Family and domain databases

Gene3Di 3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase."
    Lermontova I., Kruse E., Mock H.-P., Grimm B.
    Proc. Natl. Acad. Sci. U.S.A. 94:8895-8900(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, SUBCELLULAR LOCATION.
    Strain: cv. SR1.
  2. "Molecular characterization of photomixotrophic cultured tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides."
    Watanabe N., Che F., Iwano M., Takayama S., Nakano T., Yoshida S., Isogai A.
    Plant Physiol. 118:751-758(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Samsun NN.
  3. "The molecular basis of photobleaching herbicide resistance in Tobacco."
    Horikoshi M., Mametsuka K., Hirooka T.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. SR1.
  4. "Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis."
    Koch M., Breithaupt C., Kiefersauer R., Freigang J., Huber R., Messerschmidt A.
    EMBO J. 23:1720-1728(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) IN COMPLEX WITH SYNTHETIC INHIBITOR AND FAD, IDENTIFICATION BY MASS SPECTROMETRY, COFACTOR.

Entry informationi

Entry nameiPPOM_TOBAC
AccessioniPrimary (citable) accession number: O24164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3