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O24163 (PPOC_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protoporphyrinogen oxidase, chloroplastic

Short name=PPO
EC=1.3.3.4
Alternative name(s):
Protoporphyrinogen IX oxidase isozyme I
Short name=PPO I
Short name=PPX I
Gene names
Name:PPXI
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Ref.1

Catalytic activity

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactor

Binds 1 FAD per subunit By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast Ref.1.

Developmental stage

Expressed in expanding premature leaves. Decreased expression in oldest leaves. Expressed at very low level in roots. Ref.1

Induction

Oscillating expression during diurnal growth. Maximal expression in the dark period. Ref.1

Sequence similarities

Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
   Biological processChlorophyll biosynthesis
Heme biosynthesis
Porphyrin biosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processchlorophyll biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

protoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxygen-dependent protoporphyrinogen oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Potential
Chain51 – 548498Protoporphyrinogen oxidase, chloroplastic
PRO_0000013326

Regions

Nucleotide binding78 – 836FAD By similarity
Nucleotide binding101 – 1022FAD By similarity
Nucleotide binding123 – 1264FAD By similarity
Nucleotide binding522 – 5243FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
O24163 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 66892E78FB8A3E30

FASTA54859,230
        10         20         30         40         50         60 
MTTTPIANHP NIFTHQSSSS PLAFLNRTSF IPFSSISKRN SVNCNGWRTR CSVAKDYTVP 

        70         80         90        100        110        120 
SSAVDGGPAA ELDCVIVGAG ISGLCIAQVM SANYPNLMVT EARDRAGGNI TTVERDGYLW 

       130        140        150        160        170        180 
EEGPNSFQPS DPMLTMAVDC GLKDDLVLGD PNAPRFVLWK GKLRPVPSKL TDLAFFDLMS 

       190        200        210        220        230        240 
IPGKLRAGFG AIGLRPSPPG HEESVEQFVR RNLGGEVFER LIEPFCSGVY AGDPSKLSMK 

       250        260        270        280        290        300 
AAFGKVWKLE ETGGSIIGGT FKAIKERSST PKAPRDPRLP KPKGQTVGSF RKGLRMLPDA 

       310        320        330        340        350        360 
ISARLGSKLK LSWKLSSITK SEKGGYHLTY ETPEGVVSLQ SRSIVMTVPS YVASNILRPL 

       370        380        390        400        410        420 
SVAAADALSN FYYPPVGAVT ITYPQEAIRD ERLVDGELKG FGQLHPRTQG VETLGTIYSS 

       430        440        450        460        470        480 
SLFPNRAPKG RVLLLNYIGG AKNPEILSKT ESQLVEVVDR DLRKMLIKPK AQDPLVVGVR 

       490        500        510        520        530        540 
VWPQAIPQFL VGHLDTLSTA KAAMNDNGLE GLFLGGNYVS GVALGRCVEG AYEVASEVTG 


FLSRYAYK 

« Hide

References

[1]"Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase."
Lermontova I., Kruse E., Mock H.-P., Grimm B.
Proc. Natl. Acad. Sci. U.S.A. 94:8895-8900(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, SUBCELLULAR LOCATION.
Strain: cv. SR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13465 mRNA. Translation: CAA73865.1.
PIRT04058.

3D structure databases

ProteinModelPortalO24163.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-11760.
BRENDA1.3.3.4. 3645.
UniPathwayUPA00251; UER00324.
UPA00668.

Family and domain databases

Gene3D3.90.660.20. 1 hit.
InterProIPR002937. Amino_oxidase.
IPR004572. Protoporphyrinogen_oxidase.
IPR027418. Protoporphyrinogen_oxidase_C.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00562. proto_IX_ox. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPOC_TOBAC
AccessionPrimary (citable) accession number: O24163
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways