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Protein

Protoporphyrinogen oxidase, chloroplastic

Gene

PPXI

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 836FADBy similarity
Nucleotide bindingi101 – 1022FADBy similarity
Nucleotide bindingi123 – 1264FADBy similarity
Nucleotide bindingi522 – 5243FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11760.
BRENDAi1.3.3.4. 3645.
UniPathwayiUPA00251; UER00324.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase, chloroplastic (EC:1.3.3.4)
Short name:
PPO
Alternative name(s):
Protoporphyrinogen IX oxidase isozyme I
Short name:
PPO I
Short name:
PPX I
Gene namesi
Name:PPXI
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050ChloroplastSequence AnalysisAdd
BLAST
Chaini51 – 548498Protoporphyrinogen oxidase, chloroplasticPRO_0000013326Add
BLAST

Expressioni

Developmental stagei

Expressed in expanding premature leaves. Decreased expression in oldest leaves. Expressed at very low level in roots.1 Publication

Inductioni

Oscillating expression during diurnal growth. Maximal expression in the dark period.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO24163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTPIANHP NIFTHQSSSS PLAFLNRTSF IPFSSISKRN SVNCNGWRTR
60 70 80 90 100
CSVAKDYTVP SSAVDGGPAA ELDCVIVGAG ISGLCIAQVM SANYPNLMVT
110 120 130 140 150
EARDRAGGNI TTVERDGYLW EEGPNSFQPS DPMLTMAVDC GLKDDLVLGD
160 170 180 190 200
PNAPRFVLWK GKLRPVPSKL TDLAFFDLMS IPGKLRAGFG AIGLRPSPPG
210 220 230 240 250
HEESVEQFVR RNLGGEVFER LIEPFCSGVY AGDPSKLSMK AAFGKVWKLE
260 270 280 290 300
ETGGSIIGGT FKAIKERSST PKAPRDPRLP KPKGQTVGSF RKGLRMLPDA
310 320 330 340 350
ISARLGSKLK LSWKLSSITK SEKGGYHLTY ETPEGVVSLQ SRSIVMTVPS
360 370 380 390 400
YVASNILRPL SVAAADALSN FYYPPVGAVT ITYPQEAIRD ERLVDGELKG
410 420 430 440 450
FGQLHPRTQG VETLGTIYSS SLFPNRAPKG RVLLLNYIGG AKNPEILSKT
460 470 480 490 500
ESQLVEVVDR DLRKMLIKPK AQDPLVVGVR VWPQAIPQFL VGHLDTLSTA
510 520 530 540
KAAMNDNGLE GLFLGGNYVS GVALGRCVEG AYEVASEVTG FLSRYAYK
Length:548
Mass (Da):59,230
Last modified:December 31, 1997 - v1
Checksum:i66892E78FB8A3E30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13465 mRNA. Translation: CAA73865.1.
PIRiT04058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13465 mRNA. Translation: CAA73865.1.
PIRiT04058.

3D structure databases

ProteinModelPortaliO24163.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2366455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
UPA00668.
BioCyciMetaCyc:MONOMER-11760.
BRENDAi1.3.3.4. 3645.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase."
    Lermontova I., Kruse E., Mock H.-P., Grimm B.
    Proc. Natl. Acad. Sci. U.S.A. 94:8895-8900(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, SUBCELLULAR LOCATION.
    Strain: cv. SR1.

Entry informationi

Entry nameiPPOC_TOBAC
AccessioniPrimary (citable) accession number: O24163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: December 31, 1997
Last modified: January 6, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.