Reviewed, UniProtKB/Swiss-Prot O24146 (4CL2_TOBAC)

Names and origin

Protein names

Recommended name:
    4-coumarate--CoA ligase 2
      Short name=4CL 2
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 2

Gene names

Name:

4CL2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	4-coumarate--CoA ligase 2		PRO_0000193041

Sequences

	Sequence		Length	Mass (Da)
	O24146-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CB5579AEDFCFC003	FASTA	542	59,480
	10 20 30 40 50 60 MEKDTKQVDI IFRSKLPDIY IPNHLPLHSY CFENISEFSS RPCLINGANK QIYTYADVEL 70 80 90 100 110 120 NSRKVAAGLH KQGIQPKDTI MILLPNSPEF VFAFIGASYL GAISTMANPL FTPAEVVKQA 130 140 150 160 170 180 KASSAKIIVT QACHVNKVKD YAFENDVKII CIDSAPEGCL HFSVLTQANE HDIPEVEIQP 190 200 210 220 230 240 DDVVALPYSS GTTGLPKGVM LTHKGLVTSV AQQVDGENPN LYIHSEDVML CVLPLFHIYS 250 260 270 280 290 300 LNSVLLCGLR VGAAILIMQK FDIVSFLELI QRYKVTIGPF VPPIVLAIAK SPMVDDYDLS 310 320 330 340 350 360 SVRTVMSGAA PLGKELEDTV RAKFPNAKLG QGYGMTEAGP VLAMCLAFAK EPFEIKSGAC 370 380 390 400 410 420 GTVVRNAEMK IVDPKTGNSL PRNQSGEICI RGDQIMKGYL NDPEATARTI DKEGWLYTGD 430 440 450 460 470 480 IGYIDDDDEL FIVDRLKELI KYKGFQVAPA ELEALLLNHP NISDAAVVPM KDEQAGEVPV 490 500 510 520 530 540 AFVVRSNGST ITEDEVKDFI SKQVIFYKRI KRVFFVDAIP KSPSGKILRK DLRAKLAAGL PN

References

[1]	"Two divergent members of a tobacco 4-coumarate:coenzyme A ligase (4CL) gene family. cDNA structure, gene inheritance and expression, and properties of recombinant proteins." Lee D., Douglas C.J. Plant Physiol. 112:193-205(1996) [PubMed: 8819324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U50846 mRNA. Translation: AAB18638.1.
PIR	T03789.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.2.1.12. 298.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL2_TOBAC

Accession

Primary (citable) accession number: O24146

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)