O24143 (NDUS8_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial EC=1.6.5.3 EC=1.6.99.3
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. May donate electrons to ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion Probable.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Mitochondrion By similarity
	Chain	43 – 230	188	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial		PRO_0000020018
Regions
	Domain	122 – 151	30	4Fe-4S ferredoxin-type 1
	Domain	161 – 190	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	131	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	134	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	137	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	141	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	170	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	173	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	176	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	180	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O24143 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: EA4AE7C205213412	FASTA	230	26,354
	10 20 30 40 50 60 MAAILARKSL SALRSRQLVL AGQAWQQGAN TSNGTLLGTR TFATNDSFST DKDDEEREQL 70 80 90 100 110 120 AKELSKDWNS VFEQQINTLF LTEMVRGLML TLKYFFEKKV TINYPFEKGP LSPRFRGEQP 130 140 150 160 170 180 LRRYPTGEER CIACKLCEAI CPAQAITIEA EAREDGSRRT TRYDIDMTKC IYCGFCQEAC 190 200 210 220 230 PVDAIVEGPN FEFATETHEE LLYDKEKLLE NGDRWETEIA ENLRSESLYR

References

[1]	"The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants." Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L. Mol. Gen. Genet. 253:448-454(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Virginia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09109 mRNA. Translation: CAA70326.1.
3D structure databases
ProteinModelPortal	O24143.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1060.10. 1 hit.
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012285. Fum_reductase_C. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF12838. Fer4_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS8_TOBAC

Accession

Primary (citable) accession number: O24143

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program