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O24143 (NDUS8_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. May donate electrons to ubiquinone. HAMAP-Rule MF_01351

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01351

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP-Rule MF_01351

Subunit structure

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Subcellular location

Mitochondrion Probable HAMAP-Rule MF_01351.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4242Mitochondrion By similarity
Chain43 – 230188NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial HAMAP-Rule MF_01351
PRO_0000020018

Regions

Domain122 – 151304Fe-4S ferredoxin-type 1
Domain161 – 190304Fe-4S ferredoxin-type 2

Sites

Metal binding1311Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1341Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1371Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1411Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1701Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1731Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1761Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1801Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O24143 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: EA4AE7C205213412

FASTA23026,354
        10         20         30         40         50         60 
MAAILARKSL SALRSRQLVL AGQAWQQGAN TSNGTLLGTR TFATNDSFST DKDDEEREQL 

        70         80         90        100        110        120 
AKELSKDWNS VFEQQINTLF LTEMVRGLML TLKYFFEKKV TINYPFEKGP LSPRFRGEQP 

       130        140        150        160        170        180 
LRRYPTGEER CIACKLCEAI CPAQAITIEA EAREDGSRRT TRYDIDMTKC IYCGFCQEAC 

       190        200        210        220        230 
PVDAIVEGPN FEFATETHEE LLYDKEKLLE NGDRWETEIA ENLRSESLYR 

« Hide

References

[1]"The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants."
Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L.
Mol. Gen. Genet. 253:448-454(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Virginia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09109 mRNA. Translation: CAA70326.1.

3D structure databases

ProteinModelPortalO24143.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS8_TOBAC
AccessionPrimary (citable) accession number: O24143
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families