ID   MDHC_MESCR              Reviewed;         332 AA.
AC   O24047;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 67.
DE   RecName: Full=Malate dehydrogenase, cytoplasmic;
DE            EC=1.1.1.37;
GN   Name=MDH;
OS   Mesembryanthemum crystallinum (Common ice plant).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Caryophyllales; Aizoaceae; Mesembryanthemum.
OX   NCBI_TaxID=3544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Green leaf;
RX   MEDLINE=98019082; PubMed=9358050; DOI=10.1016/S0378-1119(97)00361-2;
RA   Ocheretina O., Scheibe R.;
RT   "Cloning and sequence analysis of cDNAs encoding plant cytosolic
RT   malate dehydrogenase.";
RL   Gene 199:145-148(1997).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
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DR   EMBL; X96539; CAA65384.1; -; mRNA.
DR   PIR; T12433; T12433.
DR   HSSP; P11708; 4MDH.
DR   BRENDA; 1.1.1.37; 2210.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR011274; Malate_DH_NAD-dep_euk.
DR   InterPro; IPR010945; Malate_DH_SF1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR23382; MDH_SF1; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   ProDom; PD003052; Mal_dehydrog; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   TIGRFAMs; TIGR01758; MDH_euk_cyt; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN         1    332       Malate dehydrogenase, cytoplasmic.
FT                                /FTId=PRO_0000113417.
FT   NP_BIND      12     18       NAD (By similarity).
FT   NP_BIND     130    132       NAD (By similarity).
FT   ACT_SITE    188    188       Proton acceptor (By similarity).
FT   BINDING      93     93       Substrate (By similarity).
FT   BINDING      99     99       Substrate (By similarity).
FT   BINDING     106    106       NAD (By similarity).
FT   BINDING     113    113       NAD (By similarity).
FT   BINDING     132    132       Substrate (By similarity).
FT   BINDING     163    163       Substrate (By similarity).
SQ   SEQUENCE   332 AA;  35498 MW;  E5AF1F3013CEEAE6 CRC64;
     MAVEPLRVLV TGAAGQIGYA LVPMIARGIM LGANQPVILH MLDIPPAAEA LNGVKMELVD
     AAFPLLKGVV ATTDAAEACK GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KAQASALEQH
     AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNISCLTRLD HNRALGQISE RLNVQVSDVK
     NVIIWGNHSS TQYPDVNHAT VKTQGVDKPV RELVADDAWL NGEFITTVQQ RGAAIIKARK
     LSSALSAASS ACDHIHDWVL GTPEGTWVSM GVYSDGSYNV PAGIIYSFPV TCKNGEWTIV
     QGLPIDDDSR KKMDATAAEL VEEKTLAYSC LT
//