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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH

Organism
Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstratePROSITE-ProRule annotation
Binding sitei99 – 991SubstratePROSITE-ProRule annotation
Binding sitei106 – 1061NADBy similarity
Binding sitei113 – 1131NADBy similarity
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei163 – 1631SubstratePROSITE-ProRule annotation
Active sitei188 – 1881Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADBy similarity
Nucleotide bindingi130 – 1323NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.37. 3238.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Gene namesi
Name:MDH
OrganismiMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Taxonomic identifieri3544 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Malate dehydrogenase, cytoplasmicPRO_0000113417Add
BLAST

Proteomic databases

PRIDEiO24047.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO24047.
SMRiO24047. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVEPLRVLV TGAAGQIGYA LVPMIARGIM LGANQPVILH MLDIPPAAEA
60 70 80 90 100
LNGVKMELVD AAFPLLKGVV ATTDAAEACK GVNVAVMVGG FPRKEGMERK
110 120 130 140 150
DVMSKNVSIY KAQASALEQH AAPNCKVLVV ANPANTNALI LKEFAPSIPE
160 170 180 190 200
KNISCLTRLD HNRALGQISE RLNVQVSDVK NVIIWGNHSS TQYPDVNHAT
210 220 230 240 250
VKTQGVDKPV RELVADDAWL NGEFITTVQQ RGAAIIKARK LSSALSAASS
260 270 280 290 300
ACDHIHDWVL GTPEGTWVSM GVYSDGSYNV PAGIIYSFPV TCKNGEWTIV
310 320 330
QGLPIDDDSR KKMDATAAEL VEEKTLAYSC LT
Length:332
Mass (Da):35,498
Last modified:January 1, 1998 - v1
Checksum:iE5AF1F3013CEEAE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96539 mRNA. Translation: CAA65384.1.
PIRiT12433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96539 mRNA. Translation: CAA65384.1.
PIRiT12433.

3D structure databases

ProteinModelPortaliO24047.
SMRiO24047. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO24047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.1.1.37. 3238.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of cDNAs encoding plant cytosolic malate dehydrogenase."
    Ocheretina O., Scheibe R.
    Gene 199:145-148(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Green leaf.

Entry informationi

Entry nameiMDHC_MESCR
AccessioniPrimary (citable) accession number: O24047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.