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Reviewed, UniProtKB/Swiss-Prot O24047 (MDHC_MESCR)

Last modified February 9, 2010. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37
Gene names
Name: MDH
OrganismMesembryanthemum crystallinum (Common ice plant)
Taxonomic identifier3544 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Aizoaceae › Mesembryanthemum

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Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

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Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Malate dehydrogenase, cytoplasmic
PRO_0000113417

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding130 – 1323NAD By similarity

Sites

Active site1881Proton acceptor By similarity
Binding site931Substrate By similarity
Binding site991Substrate By similarity
Binding site1061NAD By similarity
Binding site1131NAD By similarity
Binding site1321Substrate By similarity
Binding site1631Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
O24047-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: E5AF1F3013CEEAE6

FASTA33235,498
        10         20         30         40         50         60 
MAVEPLRVLV TGAAGQIGYA LVPMIARGIM LGANQPVILH MLDIPPAAEA LNGVKMELVD 

        70         80         90        100        110        120 
AAFPLLKGVV ATTDAAEACK GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KAQASALEQH 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNISCLTRLD HNRALGQISE RLNVQVSDVK 

       190        200        210        220        230        240 
NVIIWGNHSS TQYPDVNHAT VKTQGVDKPV RELVADDAWL NGEFITTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
LSSALSAASS ACDHIHDWVL GTPEGTWVSM GVYSDGSYNV PAGIIYSFPV TCKNGEWTIV 

       310        320        330 
QGLPIDDDSR KKMDATAAEL VEEKTLAYSC LT

« Hide

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References

[1]"Cloning and sequence analysis of cDNAs encoding plant cytosolic malate dehydrogenase."
Ocheretina O., Scheibe R.
Gene 199:145-148(1997) [PubMed: 9358050] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Green leaf.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X96539 mRNA. Translation: CAA65384.1.
PIRT12433.

3D structure databases

SMRO24047. Positions 4-332.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.37. 2210.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_SF1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR23382. MDH_SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHC_MESCR
AccessionPrimary (citable) accession number: O24047
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: February 9, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents