O24035 (PANC_LOTJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantoate--beta-alanine ligase
EC=6.3.2.1

Alternative name(s):
Pantoate-activating enzyme
Pantothenate synthetase

Gene names

Name:

PANC

Organism

Lotus japonicus (Lotus corniculatus var. japonicus)

Taxonomic identifier

34305 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Loteae › Lotus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.
Subunit structure	Homodimer Potential.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Expressed at low levels in leaf and root. Ref.1
Sequence similarities	Belongs to the pantothenate synthetase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.8. Activity decreases sharply with increasing acidity and is null at pH 7. There is only a slight decrease toward higher pH.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Propeptide	2	1	Removed; partial		PRO_0000023005
	Chain	3 – 308	306	Pantoate--beta-alanine ligase		PRO_0000023006

Sequences

	Sequence		Length	Mass (Da)
	O24035 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 13344F2A8508D2D0	FASTA	308	34,240
	10 20 30 40 50 60 MAPMVISDKD EMRKWSRSMR SQGKLIALVP TMGFLHEGHL SLVRDAHNHA DLVAVSIYVN 70 80 90 100 110 120 PGQFSPTEDL SAYPSDFQGD LQKLMSVPGG VDVVFHPHNL YDYGGDGGDA VAECGGDGVV 130 140 150 160 170 180 SCVDRRSGFG HETWVRAEKL EKPLCGKSRP VFFRGVATIV TKLFNIVEPD VAVFGKKDYQ 190 200 210 220 230 240 QWKIIQRMVR DLDFSIKVIG SEVIREKDGL AMSSRNVYLS PEEREKAVSI NKSLFRAKSA 250 260 270 280 290 300 AEDGQIHCEK LINLVVQSIT EAGGRIDYAE IVDQNNLEKV EWIKGPVVFC VSAWFGKARL IDNIEINL

References

[1]

"The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice)." Genschel U., Powell C.A., Abell C., Smith A.G. Biochem. J. 341:669-678(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17, TISSUE SPECIFICITY. Strain: cv. Gifu / B-129. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10252 mRNA. Translation: CAA71302.1.
UniGene	Lja.12058.
3D structure databases
ProteinModelPortal	O24035.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-9445.
BRENDA	6.3.2.1. 3076.
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_LOTJA

Accession

Primary (citable) accession number: O24035

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program