Reviewed, UniProtKB/Swiss-Prot O23920 (HPPD_DAUCA)

Names and origin

Protein names	Recommended name:     4-hydroxyphenylpyruvate dioxygenase     EC=1.13.11.27 Alternative name(s):     4-hydroxyphenylpyruvic acid oxidase       Short name=HPPDase       Short name=4HPPD       Short name=HPD
Organism	Daucus carota (Carrot)
Taxonomic identifier	4039 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › Scandiceae › Daucinae › Daucus

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 3/6. Cofactor biosynthesis; prenylquinone biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088398
Sites
	Metal binding	219	1	Iron By similarity
	Metal binding	301	1	Iron By similarity
	Metal binding	387	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23920-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A484AFFA267F7BF7	FASTA	442	48,148
	10 20 30 40 50 60 MGKKQSEAEI LSSNSSNTSP ATFKLVGFNN FVRANPKSDH FAVKRFHHIE FWCGDATNTS 70 80 90 100 110 120 RRFSWGLGMP LVAKSDLSTG NSVHASYLVR SANLSFVFTA PYSPSTTTSS GSAAIPSFSA 130 140 150 160 170 180 SGFHSFAAKH GLAVRAIALE VADVAAAFEA SVARGARPAS APVELDDQAW LAEVELYGDV 190 200 210 220 230 240 VLRFVSFGRE EGLFLPGFEA VEGTASFPDL DYGIRRLDHA VGNVTELGPV VEYIKGFTGF 250 260 270 280 290 300 HEFAEFTAED VGTLESGLNS VVLANNEEMV LLPLNEPVYG TKRKSQIQTY LEHNEGAGVQ 310 320 330 340 350 360 HLALVSEDIF RTLREMRKRS CLGGFEFMPS PPPTYYKNLK NRVGDVLSDE QIKECEDLGI 370 380 390 400 410 420 LVDRDDQGTL LQIFTKPVGD RPTLFIEIIQ RVGCMLKDDA GQMYQKGGCG GFGKGNFSEL 430 440 FKSIEEYEKT LEAKQITGSA AA

References

[1]

"Subcellular localization and purification of a p-hydroxyphenylpyruvate dioxygenase from cultured carrot cells and characterization of the corresponding cDNA." Garcia I., Rodgers M., Lenne C., Rolland A., Sailland A., Matringe M. Biochem. J. 325:761-769(1997) [PubMed: 9271098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U87257 mRNA. Translation: AAC49815.1.
PIR	T14353.
3D structure databases
SMR	O23920. Positions 32-430.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.27. 1081.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_bleo-R_dOase. [Graphical view]
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_DAUCA

Accession

Primary (citable) accession number: O23920

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)