Reviewed, UniProtKB/Swiss-Prot O23877 (FENR3_ORYSJ)

Names and origin

Protein names

Recommended name:
    Ferredoxin--NADP reductase, embryo isozyme, chloroplastic
      Short name=FNR
    EC=1.18.1.2

Gene names

Ordered Locus Names:	Os07g0147900, LOC_Os07g05400
ORF Names:	P0022E03.21-1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Is involved in nitrate assimilation.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Pathway	Energy metabolism; photosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O23877-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O23877-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-218: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 62	62	Chloroplast
	Chain	63 – 378	316	Ferredoxin--NADP reductase, embryo isozyme, chloroplastic		PRO_0000019417
Regions
	Domain	93 – 221	129	FAD-binding FR-type
	Nucleotide binding	153 – 156	4	FAD By similarity
	Nucleotide binding	174 – 176	3	FAD By similarity
	Nucleotide binding	195 – 197	3	FAD By similarity
	Nucleotide binding	269 – 270	2	NADP By similarity
	Nucleotide binding	299 – 300	2	NADP By similarity
	Nucleotide binding	337 – 338	2	NADP By similarity
Sites
	Binding site	156	1	NADP By similarity
	Binding site	176	1	NADP By similarity
	Binding site	180	1	FAD By similarity
	Binding site	237	1	FAD By similarity
	Binding site	237	1	NADP; via amide nitrogen By similarity
	Binding site	309	1	NADP By similarity
	Binding site	376	1	NADP By similarity
Natural variations
	Alternative sequence	1 – 218	218	Missing in isoform 2.		VSP_017442

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CDDB2CFBC4689145	FASTA	378	41,815
	10 20 30 40 50 60 MASALGAQAS VAAPIGAGGY GRSSSSKGSN TVNFCNKSWI GTTLAWESKA LKSRHMNKIF 70 80 90 100 110 120 SMSVQQASKS KVAVKPLELD NAKEPPLNLY KPKEPYTATI VSVERLVGPK APGETCHIVI 130 140 150 160 170 180 DHGGNVPYWE GQSYGVIPPG ENPKKPGSPN TVRLYSIAST RYGDSFDGKT ASLCVRRAVY 190 200 210 220 230 240 YDPETGKEDP TKKGICSNFL CDSKPGDKVQ ITGPSGKIML LPEDDPNATH IMIATGTGVA 250 260 270 280 290 300 PYRGYLRRMF MEDVPSFKFG GLAWLFLGVA NTDSLLYDEE FTNYLQQYPD NFRYDKALSR 310 320 330 340 350 360 EQKNKNGGKM YVQDKIEEYS DEIFKLLDGG AHIYFCGLKG MMPGIQDTLK RVAEQRGESW 370 EQKLSQLKKN KQWHVEVY
	Isoform 2. Checksum: 760EA413339EBCAD	FASTA	160	18,608
	10 20 30 40 50 60 MLLPEDDPNA THIMIATGTG VAPYRGYLRR MFMEDVPSFK FGGLAWLFLG VANTDSLLYD 70 80 90 100 110 120 EEFTNYLQQY PDNFRYDKAL SREQKNKNGG KMYVQDKIEE YSDEIFKLLD GGAHIYFCGL 130 140 150 160 KGMMPGIQDT LKRVAEQRGE SWEQKLSQLK KNKQWHVEVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of a cDNA encoding rice embryo ferredoxin-NADP+ oxidoreductase." Aoki H., Kumada H.O., Masumura T., Tanaka K., Ida S. Plant Gene Register PGR96-115 Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Nipponbare.
[2]	"Oryza sativa nipponbare chromosome 7 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Nipponbare.
[4]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 281-289. Strain: cv. Nipponbare. Tissue: Stem.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87547 mRNA. Translation: BAA13417.1. AP004263 Genomic DNA. Translation: BAC83340.1. AK072768 mRNA. No translation available.
PIR	T02977.
RefSeq	NP_001058890.1.
UniGene	Os.1
3D structure databases
SMR	O23877. Positions 68-378.
ModBase	Search...
Genome annotation databases
GeneID	4342399.
KEGG	osa:4342399.
Organism-specific databases
Gramene	O23877.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012146. Frd-NADP+_RD. IPR015701. FRD_Red. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR19384:SF1. FRD_Red. 1 hit.
Pfam	PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000361. Frd-NADP+_RD. 1 hit.
PRINTS	PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FENR3_ORYSJ

Accession

Primary (citable) accession number: O23877
Secondary accession number(s): Q6ZF67

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)