Reviewed, UniProtKB/Swiss-Prot O23814 (GPX4_SPIOL)

Names and origin

Protein names	Recommended name:     Probable phospholipid hydroperoxide glutathione peroxidase       Short name=PHGPx     EC=1.11.1.12
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: InterPro phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Probable phospholipid hydroperoxide glutathione peroxidase		PRO_0000066635
Sites
	Active site	44	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23814-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0DA82AAB80BAD476	FASTA	171	19,048
	10 20 30 40 50 60 MASDSSAQPK SVHEFVVRDA RGNDVDLSIY KGKVLLIVNV ASQCGLTNSN YTEMTELYEK 70 80 90 100 110 120 YRELGLEILA FPCNQFGNQE PGSNEEVLEF ACTRFKAEYP IFDKVDVNGS NAAPIYKFLK 130 140 150 160 170 SSKGGLFGDG LKWNFTKFLV DKDGNVVDRY APTTSPKSIE KDVKKLLGIQ K

References

[1]

"Molecular cloning and characterization of a cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase from spinach." Sugimoto M., Furui S., Suzuki Y. Biosci. Biotechnol. Biochem. 61:1379-1381(1997) [PubMed: 9301122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. King of Denmark.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63425 mRNA. Translation: BAA22194.1.
PIR	JC5619.
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
ModBase	Search...
Protein-protein interaction databases
IntAct	O23814. 1 interaction.
Protein family/group databases
PeroxiBase	2925. SoGPx06.
Enzyme and pathway databases
BRENDA	1.11.1.12. 286.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX4_SPIOL

Accession

Primary (citable) accession number: O23814

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)