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O23791 (BROM1_ANACO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 3, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fruit bromelain

EC=3.4.22.33
Alternative name(s):
Allergen=Ana c 2
OrganismAnanas comosus (Pineapple) (Ananas ananas)
Taxonomic identifier4615 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesBromeliaceaeAnanas

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-|-NHMec (c.f. stem bromelain).

Allergenic properties

Causes an allergic reaction in human.

Sequence similarities

Belongs to the peptidase C1 family.

Ontologies

Keywords
   DiseaseAllergen
   DomainSignal
   Molecular functionHydrolase
Protease
Thiol protease
   PTMDisulfide bond
Zymogen
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 12197 By similarity
PRO_0000045809
Chain122 – 351230Fruit bromelain
PRO_0000045810

Sites

Active site1471 By similarity
Active site2791 By similarity

Amino acid modifications

Disulfide bond144 ↔ 184 By similarity
Disulfide bond178 ↔ 217 By similarity
Disulfide bond273 ↔ 325 By similarity

Sequences

Sequence LengthMass (Da)Tools
O23791 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 33781C55144242B0

FASTA35139,055
        10         20         30         40         50         60 
MASKVQLVFL FLFLCAMWAS PSAASRDEPN DPMMKRFEEW MAEYGRVYKD DDEKMRRFQI 

        70         80         90        100        110        120 
FKNNVKHIET FNSRNENSYT LGINQFTDMT KSEFVAQYTG VSLPLNIERE PVVSFDDVNI 

       130        140        150        160        170        180 
SAVPQSIDWR DYGAVNEVKN QNPCGSCWSF AAIATVEGIY KIKTGYLVSL SEQEVLDCAV 

       190        200        210        220        230        240 
SYGCKGGWVN KAYDFIISNN GVTTEENYPY LAYQGTCNAN SFPNSAYITG YSYVRRNDER 

       250        260        270        280        290        300 
SMMYAVSNQP IAALIDASEN FQYYNGGVFS GPCGTSLNHA ITIIGYGQDS SGTKYWIVRN 

       310        320        330        340        350 
SWGSSWGEGG YVRMARGVSS SSGVCGIAMA PLFPTLQSGA NAEVIKMVSE T 

« Hide

References

[1]"Cloning and sequencing of fruit bromelain."
Muta E., Aramaki H., Takata Y., Kono A., Okamoto Y., Ota S.
Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. N67-10.
Tissue: Fruit.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14059 mRNA. Translation: BAA21849.1.
PIRT10503.

3D structure databases

ProteinModelPortalO23791.
SMRO23791. Positions 123-338.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3076. Ana c 2.0101.
694. Ana c 2.
MEROPSC01.028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.4.22.33. 333.

Family and domain databases

InterProIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERPTHR12411. PTHR12411. 1 hit.
PfamPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSPR00705. PAPAIN.
SMARTSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBROM1_ANACO
AccessionPrimary (citable) accession number: O23791
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 1, 1998
Last modified: October 3, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Allergens

Nomenclature of allergens and list of entries