O23791 (BROM1_ANACO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Fruit bromelain EC=3.4.22.33 Alternative name(s): Allergen=Ana c 2
Organism	Ananas comosus (Pineapple) (Ananas ananas)
Taxonomic identifier	4615 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Bromeliaceae › Ananas

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-|-NHMec (c.f. stem bromelain).
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 121	97	By similarity		PRO_0000045809
	Chain	122 – 351	230	Fruit bromelain		PRO_0000045810
Sites
	Active site	147	1	By similarity
	Active site	279	1	By similarity
Amino acid modifications
	Disulfide bond	144 ↔ 184		By similarity
	Disulfide bond	178 ↔ 217		By similarity
	Disulfide bond	273 ↔ 325		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23791 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 33781C55144242B0	FASTA	351	39,055
	10 20 30 40 50 60 MASKVQLVFL FLFLCAMWAS PSAASRDEPN DPMMKRFEEW MAEYGRVYKD DDEKMRRFQI 70 80 90 100 110 120 FKNNVKHIET FNSRNENSYT LGINQFTDMT KSEFVAQYTG VSLPLNIERE PVVSFDDVNI 130 140 150 160 170 180 SAVPQSIDWR DYGAVNEVKN QNPCGSCWSF AAIATVEGIY KIKTGYLVSL SEQEVLDCAV 190 200 210 220 230 240 SYGCKGGWVN KAYDFIISNN GVTTEENYPY LAYQGTCNAN SFPNSAYITG YSYVRRNDER 250 260 270 280 290 300 SMMYAVSNQP IAALIDASEN FQYYNGGVFS GPCGTSLNHA ITIIGYGQDS SGTKYWIVRN 310 320 330 340 350 SWGSSWGEGG YVRMARGVSS SSGVCGIAMA PLFPTLQSGA NAEVIKMVSE T

References

[1]	"Cloning and sequencing of fruit bromelain." Muta E., Aramaki H., Takata Y., Kono A., Okamoto Y., Ota S. Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. N67-10. Tissue: Fruit.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14059 mRNA. Translation: BAA21849.1.
PIR	T10503.
3D structure databases
ProteinModelPortal	O23791.
SMR	O23791. Positions 123-338.
ModBase	Search...
Protein family/group databases
Allergome	3076. Ana c 2.0101. 694. Ana c 2.
MEROPS	C01.028.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.4.22.33. 333.
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BROM1_ANACO

Accession

Primary (citable) accession number: O23791

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 1, 1998
Last modified:	October 3, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program