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Protein

Fruit bromelain

Gene
N/A
Organism
Ananas comosus (Pineapple) (Ananas ananas)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-|-NHMec (c.f. stem bromelain).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471By similarity
Active sitei279 – 2791By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.33. 333.

Protein family/group databases

MEROPSiC01.028.

Names & Taxonomyi

Protein namesi
Recommended name:
Fruit bromelain (EC:3.4.22.33)
Alternative name(s):
Allergen: Ana c 2
OrganismiAnanas comosus (Pineapple) (Ananas ananas)
Taxonomic identifieri4615 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesBromeliaceaeAnanas

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3076. Ana c 2.0101.
694. Ana c 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 12197By similarityPRO_0000045809Add
BLAST
Chaini122 – 351230Fruit bromelainPRO_0000045810Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi144 ↔ 184By similarity
Disulfide bondi178 ↔ 217By similarity
Disulfide bondi273 ↔ 325By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliO23791.
SMRiO23791. Positions 123-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKVQLVFL FLFLCAMWAS PSAASRDEPN DPMMKRFEEW MAEYGRVYKD
60 70 80 90 100
DDEKMRRFQI FKNNVKHIET FNSRNENSYT LGINQFTDMT KSEFVAQYTG
110 120 130 140 150
VSLPLNIERE PVVSFDDVNI SAVPQSIDWR DYGAVNEVKN QNPCGSCWSF
160 170 180 190 200
AAIATVEGIY KIKTGYLVSL SEQEVLDCAV SYGCKGGWVN KAYDFIISNN
210 220 230 240 250
GVTTEENYPY LAYQGTCNAN SFPNSAYITG YSYVRRNDER SMMYAVSNQP
260 270 280 290 300
IAALIDASEN FQYYNGGVFS GPCGTSLNHA ITIIGYGQDS SGTKYWIVRN
310 320 330 340 350
SWGSSWGEGG YVRMARGVSS SSGVCGIAMA PLFPTLQSGA NAEVIKMVSE

T
Length:351
Mass (Da):39,055
Last modified:January 1, 1998 - v1
Checksum:i33781C55144242B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14059 mRNA. Translation: BAA21849.1.
PIRiT10503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14059 mRNA. Translation: BAA21849.1.
PIRiT10503.

3D structure databases

ProteinModelPortaliO23791.
SMRiO23791. Positions 123-338.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3076. Ana c 2.0101.
694. Ana c 2.
MEROPSiC01.028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.22.33. 333.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of fruit bromelain."
    Muta E., Aramaki H., Takata Y., Kono A., Okamoto Y., Ota S.
    Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. N67-10.
    Tissue: Fruit.

Entry informationi

Entry nameiBROM1_ANACO
AccessioniPrimary (citable) accession number: O23791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.