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O23791

- BROM1_ANACO

UniProt

O23791 - BROM1_ANACO

Protein

Fruit bromelain

Gene
N/A
Organism
Ananas comosus (Pineapple) (Ananas ananas)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-|-NHMec (c.f. stem bromelain).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei147 – 1471By similarity
    Active sitei279 – 2791By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Enzyme and pathway databases

    BRENDAi3.4.22.33. 333.

    Protein family/group databases

    MEROPSiC01.028.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fruit bromelain (EC:3.4.22.33)
    Alternative name(s):
    Allergen: Ana c 2
    OrganismiAnanas comosus (Pineapple) (Ananas ananas)
    Taxonomic identifieri4615 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesBromeliaceaeAnanas

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei3076. Ana c 2.0101.
    694. Ana c 2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 12197By similarityPRO_0000045809Add
    BLAST
    Chaini122 – 351230Fruit bromelainPRO_0000045810Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi144 ↔ 184By similarity
    Disulfide bondi178 ↔ 217By similarity
    Disulfide bondi273 ↔ 325By similarity

    Keywords - PTMi

    Disulfide bond, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliO23791.
    SMRiO23791. Positions 123-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view]
    PANTHERiPTHR12411. PTHR12411. 1 hit.
    PfamiPF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00705. PAPAIN.
    SMARTiSM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view]
    PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O23791-1 [UniParc]FASTAAdd to Basket

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    MASKVQLVFL FLFLCAMWAS PSAASRDEPN DPMMKRFEEW MAEYGRVYKD    50
    DDEKMRRFQI FKNNVKHIET FNSRNENSYT LGINQFTDMT KSEFVAQYTG 100
    VSLPLNIERE PVVSFDDVNI SAVPQSIDWR DYGAVNEVKN QNPCGSCWSF 150
    AAIATVEGIY KIKTGYLVSL SEQEVLDCAV SYGCKGGWVN KAYDFIISNN 200
    GVTTEENYPY LAYQGTCNAN SFPNSAYITG YSYVRRNDER SMMYAVSNQP 250
    IAALIDASEN FQYYNGGVFS GPCGTSLNHA ITIIGYGQDS SGTKYWIVRN 300
    SWGSSWGEGG YVRMARGVSS SSGVCGIAMA PLFPTLQSGA NAEVIKMVSE 350
    T 351
    Length:351
    Mass (Da):39,055
    Last modified:January 1, 1998 - v1
    Checksum:i33781C55144242B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14059 mRNA. Translation: BAA21849.1.
    PIRiT10503.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14059 mRNA. Translation: BAA21849.1 .
    PIRi T10503.

    3D structure databases

    ProteinModelPortali O23791.
    SMRi O23791. Positions 123-338.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3076. Ana c 2.0101.
    694. Ana c 2.
    MEROPSi C01.028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 3.4.22.33. 333.

    Family and domain databases

    InterProi IPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view ]
    PANTHERi PTHR12411. PTHR12411. 1 hit.
    Pfami PF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00705. PAPAIN.
    SMARTi SM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view ]
    PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of fruit bromelain."
      Muta E., Aramaki H., Takata Y., Kono A., Okamoto Y., Ota S.
      Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. N67-10.
      Tissue: Fruit.

    Entry informationi

    Entry nameiBROM1_ANACO
    AccessioniPrimary (citable) accession number: O23791
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3