Reviewed, UniProtKB/Swiss-Prot O23735 (CYSK2_BRAJU)

Names and origin

Protein names	Recommended name:     Cysteine synthase       Short name=CSase     EC=2.5.1.47 Alternative name(s):     O-acetylserine sulfhydrylase     O-acetylserine (thiol)-lyase       Short name=OAS-TL     OAS-TL6
Organism	Brassica juncea (Leaf mustard) (Indian mustard)
Taxonomic identifier	3707 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Cysteine synthase		PRO_0000167118
Regions
	Region	183 – 187	5	Pyridoxal phosphate binding By similarity
	Compositional bias	274 – 279	6	Poly-Ala
Sites
	Binding site	79	1	Pyridoxal phosphate By similarity
	Binding site	271	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	48	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23735-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 091B5B2C0BED1EA1	FASTA	324	34,132
	10 20 30 40 50 60 MASRIGIAND VTELIGNTPL VYLNSVAEGC VGRVAAKLEM MEPCSSVKDR IGFSMISDAE 70 80 90 100 110 120 KKGLIKPGES VLIEPTSGNT GVGLAFTAAA KGYKLIITMP ASMSIERRII LLAFGVELVL 130 140 150 160 170 180 TDPAKGMKGA IAKAEEILAK TPNGYMLQQF ENPANPKIHY ETTGPEIWKG TEGKIDGFIS 190 200 210 220 230 240 GIGTGGTITG AGKYLKEQNP NVKLYGVEPV ESAILSGGKP GPHKIQGIGA GFIPNVLETN 250 260 270 280 290 300 LIDEVVQVSS DESIDMARLL AREEGLLVGI SSGAAAAAAI KLAKRPENAG KLFVAVFPSF 310 320 GERYLSTVLF DATRKEAESM TFEA

References

[1]

"cDNA cloning and expression analysis of genes encoding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L.: evidence for Cd-induction of a putative mitochondrial gamma-glutamylcysteine synthetase isoform." Schaefer H.J., Haag-Kerwer A., Rausch T.H. Plant Mol. Biol. 37:87-97(1998) [PubMed: 9620267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Vittasso. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y10847 mRNA. Translation: CAA71800.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
SMR	O23735. Positions 7-324.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.47. 21235.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK2_BRAJU

Accession

Primary (citable) accession number: O23735

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)