O23733 (CYSK1_BRAJU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cysteine synthase Short name=CSase EC=2.5.1.47 Alternative name(s): O-acetylserine (thiol)-lyase Short name=OAS-TL O-acetylserine sulfhydrylase OAS-TL4
Organism	Brassica juncea (Indian mustard) (Sinapis juncea)
Taxonomic identifier	3707 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Cysteine synthase		PRO_0000167117
Regions
	Region	181 – 185	5	Pyridoxal phosphate binding By similarity
	Compositional bias	272 – 277	6	Poly-Ala
Sites
	Binding site	77	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23733 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 28DA67631EF0ABF2	FASTA	322	33,902
	10 20 30 40 50 60 MASRIAKDVT ELIGNTPLVY LNNVAEGCVG RVAAKLEMME PCSSVKDRIG FSMISDAEQK 70 80 90 100 110 120 GLIKPGESVL IEPTSGNTGV GLAFTAAAKG YKLIITMPAS MSVERRIILL AFGVELVLTD 130 140 150 160 170 180 PAKGMKGAIA KAEEILAKTP NGYMLQQFEN PANPKIHYET TGPEIWKGTD GKIDGFVSGI 190 200 210 220 230 240 GTGGTITGAG KYLKEQNPNV KLYGVEPIES AILSGGKPGP HKIQGIGAGF IPSVLEVDLI 250 260 270 280 290 300 DEVVQVSSDE SIDMARLLAL KEGLLVGISS GAAAAAAIKL AKRPENAGKL FVAVFPSFGE 310 320 RYLSTVLFDA TRKEAESMTF QA

References

[1]

"cDNA cloning and expression analysis of genes encoding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L.: evidence for Cd-induction of a putative mitochondrial gamma-glutamylcysteine synthetase isoform." Schaefer H.J., Haag-Kerwer A., Rausch T.H. Plant Mol. Biol. 37:87-97(1998) [PubMed: 9620267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Vittasso. Tissue: Root.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10845 mRNA. Translation: CAA71798.1.
3D structure databases
ProteinModelPortal	O23733.
SMR	O23733. Positions 3-322.
ModBase	Search...
Proteomic databases
PRIDE	O23733.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK1_BRAJU

Accession

Primary (citable) accession number: O23733

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	May 31, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program