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O23732 (GSHB_BRAJU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase, chloroplastic

Short name=GSH synthetase
Short name=GSH-S
Short name=Glutathione synthase
EC=6.3.2.3
Gene names
Name:GSH2
Synonyms:GSHII
OrganismBrassica juncea (Indian mustard) (Sinapis juncea)
Taxonomic identifier3707 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast Potential.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5252Chloroplast Potential
Chain53 – 530478Glutathione synthetase, chloroplastic
PRO_0000013059

Regions

Nucleotide binding419 – 42810ATP By similarity
Nucleotide binding455 – 4584ATP By similarity
Region204 – 2074Substrate binding By similarity
Region272 – 2743Substrate binding By similarity
Region326 – 3294Substrate binding By similarity
Region517 – 5182Substrate binding By similarity

Sites

Metal binding2001Magnesium By similarity
Metal binding2021Magnesium By similarity
Metal binding4231Magnesium By similarity
Binding site1841Substrate By similarity
Binding site2001ATP By similarity
Binding site2781Substrate By similarity
Binding site3651ATP By similarity
Binding site4301ATP By similarity
Binding site4811ATP By similarity
Binding site5061Substrate By similarity
Binding site5081ATP By similarity
Binding site5141ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
O23732 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B149C24DB4F5EAFD

FASTA53059,741
        10         20         30         40         50         60 
MVGGCSSLSY SSSSTFIATT TLSSSLKLNP QSFIFHLNLR KRPPLRCLSS LTMESPKPIL 

        70         80         90        100        110        120 
DFEKFDDGFV QKLVYDALVW SSLHGLVVGD KTHQRSGTVP GVGMMHAPIA LLPTPFPESY 

       130        140        150        160        170        180 
WNQACEVAPI FNELVDRISL DGKFIQDSLS RTRKADVFTS RLLEIHSKML ESNKREEIRL 

       190        200        210        220        230        240 
GLHRSDYMLD EETKSLLQIE MNTISCSFPG FGRLVTELHQ SLLRSHGDHL GLDSKRVPRN 

       250        260        270        280        290        300 
GSTSQFADAM AKAWLEYNNP RAVVMVVVQP DERNMYDQHW LSSVLREKHN IVTIRKSLAE 

       310        320        330        340        350        360 
VETEGRVHED GTLTVGGQAV SVVYYRSGYT PRDYPSESEW NARLLIEQSS AVKCPSIAYH 

       370        380        390        400        410        420 
LAGTKKIQQE LAKPGVLERF MDNKDDIAKL RKCFAGLWSL DDPEIIKKAI EKPELFVMKP 

       430        440        450        460        470        480 
QREGGGNNIY GDDVRENLLR LQREGEEENA AYILMQRIFP KVSNVFLLRD GVYHKDQAIS 

       490        500        510        520        530 
ELGVYGAYLR NKERVIINEH SGYLMRTKVS SSDEGGVAAG YAVLDSIYLN 

« Hide

References

[1]"cDNA cloning and expression analysis of genes encoding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L.: evidence for Cd-induction of a putative mitochondrial gamma-glutamylcysteine synthetase isoform."
Schaefer H.J., Haag-Kerwer A., Rausch T.H.
Plant Mol. Biol. 37:87-97(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Root.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10984 mRNA. Translation: CAA71878.1.

3D structure databases

ProteinModelPortalO23732.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSHB_BRAJU
AccessionPrimary (citable) accession number: O23732
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways