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Protein

Glutathione synthetase, chloroplastic

Gene

GSH2

Organism
Brassica juncea (Indian mustard) (Sinapis juncea)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase, chloroplastic (GSH1)
  2. Glutathione synthetase, chloroplastic (GSH2)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei184SubstrateBy similarity1
Metal bindingi200MagnesiumBy similarity1
Binding sitei200ATPBy similarity1
Metal bindingi202MagnesiumBy similarity1
Binding sitei278SubstrateBy similarity1
Binding sitei365ATPBy similarity1
Metal bindingi423MagnesiumBy similarity1
Binding sitei430ATPBy similarity1
Binding sitei481ATPBy similarity1
Binding sitei506SubstrateBy similarity1
Binding sitei508ATPBy similarity1
Binding sitei514ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi419 – 428ATPBy similarity10
Nucleotide bindingi455 – 458ATPBy similarity4

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase, chloroplastic (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Short name:
Glutathione synthase
Gene namesi
Name:GSH2
Synonyms:GSHII
OrganismiBrassica juncea (Indian mustard) (Sinapis juncea)
Taxonomic identifieri3707 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000001305953 – 530Glutathione synthetase, chloroplasticAdd BLAST478

Proteomic databases

PRIDEiO23732

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliO23732
SMRiO23732
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni204 – 207Substrate bindingBy similarity4
Regioni272 – 274Substrate bindingBy similarity3
Regioni326 – 329Substrate bindingBy similarity4
Regioni517 – 518Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

CDDicd00228 eu-GS, 1 hit
Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 3 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGGCSSLSY SSSSTFIATT TLSSSLKLNP QSFIFHLNLR KRPPLRCLSS
60 70 80 90 100
LTMESPKPIL DFEKFDDGFV QKLVYDALVW SSLHGLVVGD KTHQRSGTVP
110 120 130 140 150
GVGMMHAPIA LLPTPFPESY WNQACEVAPI FNELVDRISL DGKFIQDSLS
160 170 180 190 200
RTRKADVFTS RLLEIHSKML ESNKREEIRL GLHRSDYMLD EETKSLLQIE
210 220 230 240 250
MNTISCSFPG FGRLVTELHQ SLLRSHGDHL GLDSKRVPRN GSTSQFADAM
260 270 280 290 300
AKAWLEYNNP RAVVMVVVQP DERNMYDQHW LSSVLREKHN IVTIRKSLAE
310 320 330 340 350
VETEGRVHED GTLTVGGQAV SVVYYRSGYT PRDYPSESEW NARLLIEQSS
360 370 380 390 400
AVKCPSIAYH LAGTKKIQQE LAKPGVLERF MDNKDDIAKL RKCFAGLWSL
410 420 430 440 450
DDPEIIKKAI EKPELFVMKP QREGGGNNIY GDDVRENLLR LQREGEEENA
460 470 480 490 500
AYILMQRIFP KVSNVFLLRD GVYHKDQAIS ELGVYGAYLR NKERVIINEH
510 520 530
SGYLMRTKVS SSDEGGVAAG YAVLDSIYLN
Length:530
Mass (Da):59,741
Last modified:January 1, 1998 - v1
Checksum:iB149C24DB4F5EAFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10984 mRNA Translation: CAA71878.1

Similar proteinsi

Entry informationi

Entry nameiGSHB_BRAJU
AccessioniPrimary (citable) accession number: O23732
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health