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O23732

- GSHB_BRAJU

UniProt

O23732 - GSHB_BRAJU

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Protein

Glutathione synthetase, chloroplastic

Gene
GSH2, GSHII
Organism
Brassica juncea (Indian mustard) (Sinapis juncea)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei184 – 1841Substrate By similarity
Metal bindingi200 – 2001Magnesium By similarity
Binding sitei200 – 2001ATP By similarity
Metal bindingi202 – 2021Magnesium By similarity
Binding sitei278 – 2781Substrate By similarity
Binding sitei365 – 3651ATP By similarity
Metal bindingi423 – 4231Magnesium By similarity
Binding sitei430 – 4301ATP By similarity
Binding sitei481 – 4811ATP By similarity
Binding sitei506 – 5061Substrate By similarity
Binding sitei508 – 5081ATP By similarity
Binding sitei514 – 5141ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi419 – 42810ATP By similarity
Nucleotide bindingi455 – 4584ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: UniProtKB-EC
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase, chloroplastic (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Short name:
Glutathione synthase
Gene namesi
Name:GSH2
Synonyms:GSHII
OrganismiBrassica juncea (Indian mustard) (Sinapis juncea)
Taxonomic identifieri3707 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Plastidchloroplast Reviewed prediction

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252Chloroplast Reviewed predictionAdd
BLAST
Chaini53 – 530478Glutathione synthetase, chloroplasticPRO_0000013059Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO23732.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni204 – 2074Substrate binding By similarity
Regioni272 – 2743Substrate binding By similarity
Regioni326 – 3294Substrate binding By similarity
Regioni517 – 5182Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23732-1 [UniParc]FASTAAdd to Basket

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MVGGCSSLSY SSSSTFIATT TLSSSLKLNP QSFIFHLNLR KRPPLRCLSS    50
LTMESPKPIL DFEKFDDGFV QKLVYDALVW SSLHGLVVGD KTHQRSGTVP 100
GVGMMHAPIA LLPTPFPESY WNQACEVAPI FNELVDRISL DGKFIQDSLS 150
RTRKADVFTS RLLEIHSKML ESNKREEIRL GLHRSDYMLD EETKSLLQIE 200
MNTISCSFPG FGRLVTELHQ SLLRSHGDHL GLDSKRVPRN GSTSQFADAM 250
AKAWLEYNNP RAVVMVVVQP DERNMYDQHW LSSVLREKHN IVTIRKSLAE 300
VETEGRVHED GTLTVGGQAV SVVYYRSGYT PRDYPSESEW NARLLIEQSS 350
AVKCPSIAYH LAGTKKIQQE LAKPGVLERF MDNKDDIAKL RKCFAGLWSL 400
DDPEIIKKAI EKPELFVMKP QREGGGNNIY GDDVRENLLR LQREGEEENA 450
AYILMQRIFP KVSNVFLLRD GVYHKDQAIS ELGVYGAYLR NKERVIINEH 500
SGYLMRTKVS SSDEGGVAAG YAVLDSIYLN 530
Length:530
Mass (Da):59,741
Last modified:January 1, 1998 - v1
Checksum:iB149C24DB4F5EAFD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10984 mRNA. Translation: CAA71878.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10984 mRNA. Translation: CAA71878.1 .

3D structure databases

ProteinModelPortali O23732.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .

Family and domain databases

Gene3Di 1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and expression analysis of genes encoding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L.: evidence for Cd-induction of a putative mitochondrial gamma-glutamylcysteine synthetase isoform."
    Schaefer H.J., Haag-Kerwer A., Rausch T.H.
    Plant Mol. Biol. 37:87-97(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Root.

Entry informationi

Entry nameiGSHB_BRAJU
AccessioniPrimary (citable) accession number: O23732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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