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O23732

- GSHB_BRAJU

UniProt

O23732 - GSHB_BRAJU

Protein

Glutathione synthetase, chloroplastic

Gene

GSH2

Organism
Brassica juncea (Indian mustard) (Sinapis juncea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei184 – 1841SubstrateBy similarity
    Metal bindingi200 – 2001MagnesiumBy similarity
    Binding sitei200 – 2001ATPBy similarity
    Metal bindingi202 – 2021MagnesiumBy similarity
    Binding sitei278 – 2781SubstrateBy similarity
    Binding sitei365 – 3651ATPBy similarity
    Metal bindingi423 – 4231MagnesiumBy similarity
    Binding sitei430 – 4301ATPBy similarity
    Binding sitei481 – 4811ATPBy similarity
    Binding sitei506 – 5061SubstrateBy similarity
    Binding sitei508 – 5081ATPBy similarity
    Binding sitei514 – 5141ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi419 – 42810ATPBy similarity
    Nucleotide bindingi455 – 4584ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: UniProtKB-EC
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase, chloroplastic (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Short name:
    Glutathione synthase
    Gene namesi
    Name:GSH2
    Synonyms:GSHII
    OrganismiBrassica juncea (Indian mustard) (Sinapis juncea)
    Taxonomic identifieri3707 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252ChloroplastSequence AnalysisAdd
    BLAST
    Chaini53 – 530478Glutathione synthetase, chloroplasticPRO_0000013059Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO23732.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni204 – 2074Substrate bindingBy similarity
    Regioni272 – 2743Substrate bindingBy similarity
    Regioni326 – 3294Substrate bindingBy similarity
    Regioni517 – 5182Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.1080.10. 1 hit.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O23732-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGGCSSLSY SSSSTFIATT TLSSSLKLNP QSFIFHLNLR KRPPLRCLSS    50
    LTMESPKPIL DFEKFDDGFV QKLVYDALVW SSLHGLVVGD KTHQRSGTVP 100
    GVGMMHAPIA LLPTPFPESY WNQACEVAPI FNELVDRISL DGKFIQDSLS 150
    RTRKADVFTS RLLEIHSKML ESNKREEIRL GLHRSDYMLD EETKSLLQIE 200
    MNTISCSFPG FGRLVTELHQ SLLRSHGDHL GLDSKRVPRN GSTSQFADAM 250
    AKAWLEYNNP RAVVMVVVQP DERNMYDQHW LSSVLREKHN IVTIRKSLAE 300
    VETEGRVHED GTLTVGGQAV SVVYYRSGYT PRDYPSESEW NARLLIEQSS 350
    AVKCPSIAYH LAGTKKIQQE LAKPGVLERF MDNKDDIAKL RKCFAGLWSL 400
    DDPEIIKKAI EKPELFVMKP QREGGGNNIY GDDVRENLLR LQREGEEENA 450
    AYILMQRIFP KVSNVFLLRD GVYHKDQAIS ELGVYGAYLR NKERVIINEH 500
    SGYLMRTKVS SSDEGGVAAG YAVLDSIYLN 530
    Length:530
    Mass (Da):59,741
    Last modified:January 1, 1998 - v1
    Checksum:iB149C24DB4F5EAFD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10984 mRNA. Translation: CAA71878.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10984 mRNA. Translation: CAA71878.1 .

    3D structure databases

    ProteinModelPortali O23732.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 1.10.1080.10. 1 hit.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression analysis of genes encoding GSH synthesis in roots of the heavy-metal accumulator Brassica juncea L.: evidence for Cd-induction of a putative mitochondrial gamma-glutamylcysteine synthetase isoform."
      Schaefer H.J., Haag-Kerwer A., Rausch T.H.
      Plant Mol. Biol. 37:87-97(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Root.

    Entry informationi

    Entry nameiGSHB_BRAJU
    AccessioniPrimary (citable) accession number: O23732
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3