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Protein

Proteasome subunit beta type-5-A

Gene

PBE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei58NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.A10.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-5-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit E-1
Proteasome component E
Proteasome epsilon-1 chain
Gene namesi
Name:PBE1
Synonyms:PRCE
Ordered Locus Names:At1g13060
ORF Names:F3F19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G13060.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266051 – 57Removed in mature formBy similarityAdd BLAST57
ChainiPRO_000002660658 – 274Proteasome subunit beta type-5-AAdd BLAST217

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiO23717.

Expressioni

Tissue specificityi

Ubiquitous low levels, higher expression in siliques and flowers.1 Publication

Gene expression databases

ExpressionAtlasiO23717. differential.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi23103. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliO23717.
SMRiO23717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000091082.
InParanoidiO23717.
KOiK02737.
PhylomeDBiO23717.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O23717-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLDTSGFET SMPMIGFGSS SDMLDELSSV PSFDLPRTKE FDGFQKKAKD
60 70 80 90 100
MLKHAKGTTT LAFIFKGGVM VAADSRASMG GYISSQSVKK IIEINPYMLG
110 120 130 140 150
TMAGGAADCQ FWHRNLGIKC RLHELANKRR ISVSGASKLL ANMLYSYRGM
160 170 180 190 200
GLSVGTMIAG WDETGPGLYY VDNEGGRLKG DRFSVGSGSP YAYGVLDSGY
210 220 230 240 250
KYDMSVEEAS ELARRSIYHA TFRDGASGGV ASVYHVGPEG WTKLSGDDVG
260 270
ELHYHYYPVA PATAEQVMEE ATAE
Length:274
Mass (Da):29,668
Last modified:January 1, 1998 - v1
Checksum:i4CF135F32C031AA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23M → V in AAK92808 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13695 mRNA. Translation: CAA74029.1.
AF043536 mRNA. Translation: AAC32072.1.
AC007357 Genomic DNA. Translation: AAD31059.1.
CP002684 Genomic DNA. Translation: AEE28965.1.
AF348579 mRNA. Translation: AAK15550.1.
AY050871 mRNA. Translation: AAK92808.1.
AY150473 mRNA. Translation: AAN12998.1.
PIRiF86264.
RefSeqiNP_172765.1. NM_101176.3. [O23717-1]
UniGeneiAt.22436.

Genome annotation databases

EnsemblPlantsiAT1G13060.1; AT1G13060.1; AT1G13060. [O23717-1]
GeneIDi837863.
GrameneiAT1G13060.1; AT1G13060.1; AT1G13060.
KEGGiath:AT1G13060.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13695 mRNA. Translation: CAA74029.1.
AF043536 mRNA. Translation: AAC32072.1.
AC007357 Genomic DNA. Translation: AAD31059.1.
CP002684 Genomic DNA. Translation: AEE28965.1.
AF348579 mRNA. Translation: AAK15550.1.
AY050871 mRNA. Translation: AAK92808.1.
AY150473 mRNA. Translation: AAN12998.1.
PIRiF86264.
RefSeqiNP_172765.1. NM_101176.3. [O23717-1]
UniGeneiAt.22436.

3D structure databases

ProteinModelPortaliO23717.
SMRiO23717.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23103. 2 interactors.

Protein family/group databases

MEROPSiT01.A10.

Proteomic databases

PRIDEiO23717.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G13060.1; AT1G13060.1; AT1G13060. [O23717-1]
GeneIDi837863.
GrameneiAT1G13060.1; AT1G13060.1; AT1G13060.
KEGGiath:AT1G13060.

Organism-specific databases

TAIRiAT1G13060.

Phylogenomic databases

HOGENOMiHOG000091082.
InParanoidiO23717.
KOiK02737.
PhylomeDBiO23717.

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO23717.

Gene expression databases

ExpressionAtlasiO23717. differential.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB5A_ARATH
AccessioniPrimary (citable) accession number: O23717
Secondary accession number(s): Q949V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.