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Protein

Proteasome subunit alpha type-3

Gene

PAG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-3 (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit G-1
DiDi 17A-2a
Proteasome component 8
Proteasome subunit alpha type-7
Gene namesi
Name:PAG1
Synonyms:PRC8
Ordered Locus Names:At2g27020
ORF Names:T20P8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G27020.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • proteasome complex Source: TAIR
  • proteasome core complex, alpha-subunit complex Source: InterPro
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Lethal due to defect in male gametogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001240982 – 249Proteasome subunit alpha type-3Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei214N-acetylserine1 Publication1
Modified residuei220N-acetylserine1 Publication1
Cross-linki221Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO23715.
PRIDEiO23715.

PTM databases

iPTMnetiO23715.

Expressioni

Tissue specificityi

Ubiquitous low levels.2 Publications

Inductioni

Induced by the endoparasitic nematode M.incognita. Levels increase after infection in both giants cells and endodermal cells of galls. Later confined to giant cells at high levels.1 Publication

Gene expression databases

GenevisibleiO23715. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi2596. 2 interactors.
IntActiO23715. 2 interactors.
STRINGi3702.AT2G27020.1.

Structurei

3D structure databases

ProteinModelPortaliO23715.
SMRiO23715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0184. Eukaryota.
ENOG410XP01. LUCA.
HOGENOMiHOG000091086.
InParanoidiO23715.
KOiK02727.
OMAiVPDGRHF.
OrthoDBiEOG09360L97.
PhylomeDBiO23715.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIGTGYDL SVTTFSPDGR VFQIEYAAKA VDNSGTVVGI KCKDGIVMGV
60 70 80 90 100
EKLIASKMML PGSNRRIHSV HRHAGMAVAG LAADGRQIVA RAKSEARSYE
110 120 130 140 150
SVYGDAVPVK ELSERVASYV HLCTLYWWLR PFGCGVILGG YDRDGPQLYM
160 170 180 190 200
IEPSGISYRY FGAAIGKGKQ AAKTEIEKLN LSEMTCKEGV IEVAKIIYKL
210 220 230 240
HDEAKDKAFE LEMSWICEES KREHQKVPDD LLEEAKTAAK TALEEMDAD
Length:249
Mass (Da):27,377
Last modified:December 8, 2000 - v2
Checksum:iFB5BAA07AC45A25D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20R → G in CAA74027 (PubMed:9373170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13693 mRNA. Translation: CAA74027.1.
AF043528 mRNA. Translation: AAC32064.1.
AC005623 Genomic DNA. Translation: AAC77860.1.
CP002685 Genomic DNA. Translation: AEC07923.1.
AF375455 mRNA. Translation: AAK53039.1.
AY124806 mRNA. Translation: AAM70515.1.
AY088609 mRNA. Translation: AAM66932.1.
AK175942 mRNA. Translation: BAD43705.1.
AK175949 mRNA. Translation: BAD43712.1.
AJ286351 mRNA. Translation: CAB71015.1.
PIRiG84667.
RefSeqiNP_180270.1. NM_128260.5.
UniGeneiAt.22596.

Genome annotation databases

EnsemblPlantsiAT2G27020.1; AT2G27020.1; AT2G27020.
GeneIDi817244.
GrameneiAT2G27020.1; AT2G27020.1; AT2G27020.
KEGGiath:AT2G27020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13693 mRNA. Translation: CAA74027.1.
AF043528 mRNA. Translation: AAC32064.1.
AC005623 Genomic DNA. Translation: AAC77860.1.
CP002685 Genomic DNA. Translation: AEC07923.1.
AF375455 mRNA. Translation: AAK53039.1.
AY124806 mRNA. Translation: AAM70515.1.
AY088609 mRNA. Translation: AAM66932.1.
AK175942 mRNA. Translation: BAD43705.1.
AK175949 mRNA. Translation: BAD43712.1.
AJ286351 mRNA. Translation: CAB71015.1.
PIRiG84667.
RefSeqiNP_180270.1. NM_128260.5.
UniGeneiAt.22596.

3D structure databases

ProteinModelPortaliO23715.
SMRiO23715.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2596. 2 interactors.
IntActiO23715. 2 interactors.
STRINGi3702.AT2G27020.1.

PTM databases

iPTMnetiO23715.

Proteomic databases

PaxDbiO23715.
PRIDEiO23715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G27020.1; AT2G27020.1; AT2G27020.
GeneIDi817244.
GrameneiAT2G27020.1; AT2G27020.1; AT2G27020.
KEGGiath:AT2G27020.

Organism-specific databases

TAIRiAT2G27020.

Phylogenomic databases

eggNOGiKOG0184. Eukaryota.
ENOG410XP01. LUCA.
HOGENOMiHOG000091086.
InParanoidiO23715.
KOiK02727.
OMAiVPDGRHF.
OrthoDBiEOG09360L97.
PhylomeDBiO23715.

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO23715.

Gene expression databases

GenevisibleiO23715. AT.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSA3_ARATH
AccessioniPrimary (citable) accession number: O23715
Secondary accession number(s): O81151, Q680B8, Q9M3S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 8, 2000
Last modified: November 30, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.