O23715 (PSA3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-3
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit G-1
DiDi 17A-2a
Proteasome component 8
Proteasome subunit alpha type-7

Gene names

Name:	PAG1
Synonyms:	PRC8
Ordered Locus Names:	At2g27020
ORF Names:	T20P8.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.9 Ref.10
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Ubiquitous low levels. Ref.2 Ref.8
Induction	Induced by the endoparasitic nematode M.incognita. Levels increase after infection in both giants cells and endodermal cells of galls. Later confined to giant cells at high levels. Ref.8
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to cadmium ion Inferred from expression pattern. Source: TAIR response to cold Inferred from expression pattern. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Proteasome subunit alpha type-3		PRO_0000124098
Experimental info
	Sequence conflict	20	1	R → G in CAA74027. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O23715 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: FB5BAA07AC45A25D	FASTA	249	27,377
	10 20 30 40 50 60 MSSIGTGYDL SVTTFSPDGR VFQIEYAAKA VDNSGTVVGI KCKDGIVMGV EKLIASKMML 70 80 90 100 110 120 PGSNRRIHSV HRHAGMAVAG LAADGRQIVA RAKSEARSYE SVYGDAVPVK ELSERVASYV 130 140 150 160 170 180 HLCTLYWWLR PFGCGVILGG YDRDGPQLYM IEPSGISYRY FGAAIGKGKQ AAKTEIEKLN 190 200 210 220 230 240 LSEMTCKEGV IEVAKIIYKL HDEAKDKAFE LEMSWICEES KREHQKVPDD LLEEAKTAAK TALEEMDAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 20S proteasome gene family in Arabidopsis thaliana." Parmentier Y., Bouchez D., Fleck J., Genschik P. FEBS Lett. 416:281-285(1997) [PubMed: 9373170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana." Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D. Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 211-249. Strain: cv. Columbia.
[8]	"Arabidopsis thaliana genes expressed in the early compatible interaction with root-knot nematodes." Vercauteren I., van der Schueren E., Van Montagu M., Gheysen G. Mol. Plant Microbe Interact. 14:288-299(2001) [PubMed: 11277426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 219-249, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia. Tissue: Root.
[9]	"Structure and functional analyses of the 26S proteasome subunits from plants." Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D. Mol. Biol. Rep. 26:137-146(1999) [PubMed: 10363660] [Abstract] Cited for: SUBUNIT.
[10]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13693 mRNA. Translation: CAA74027.1. AF043528 mRNA. Translation: AAC32064.1. AC005623 Genomic DNA. Translation: AAC77860.1. CP002685 Genomic DNA. Translation: AEC07923.1. AF375455 mRNA. Translation: AAK53039.1. AY124806 mRNA. Translation: AAM70515.1. AY088609 mRNA. Translation: AAM66932.1. AK175942 mRNA. Translation: BAD43705.1. AK175949 mRNA. Translation: BAD43712.1. AJ286351 mRNA. Translation: CAB71015.1.
IPI	IPI00521106.
PIR	G84667.
RefSeq	NP_180270.1. NM_128260.4.
UniGene	At.22596.
3D structure databases
ProteinModelPortal	O23715.
SMR	O23715. Positions 2-245.
ModBase	Search...
Protein-protein interaction databases
IntAct	O23715. 2 interactions.
STRING	O23715.
Protein family/group databases
MEROPS	T01.977.
Proteomic databases
PRIDE	O23715.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G27020.1; AT2G27020.1; AT2G27020.
GeneID	817244.
GenomeReviews	Gene locus AT2G27020 in contig CT485783_GR.
KEGG	ath:AT2G27020.
NMPDR	fig|3702.1.peg.9730.
Organism-specific databases
TAIR	At2g27020.
Phylogenomic databases
eggNOG	KOG0184.
GeneTree	EPGT00050000005272.
HOGENOM	HBG499923.
InParanoid	O23715.
OMA	DIPLKHL.
PhylomeDB	O23715.
ProtClustDB	CLSN2683434.
Gene expression databases
Genevestigator	O23715.
GermOnline	AT2G27020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K02727.
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA3_ARATH

Accession

Primary (citable) accession number: O23715
Secondary accession number(s): O81151, Q680B8, Q9M3S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program