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O23714

- PSB2A_ARATH

UniProt

O23714 - PSB2A_ARATH

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Protein

Proteasome subunit beta type-2-A

Gene
PBD1, PRCGB, At3g22630, F16J14.18
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciARA:AT3G22630-MONOMER.

Protein family/group databases

MEROPSiT01.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-2-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit D-1
Proteasome component GB
Proteasome subunit beta type-4
Gene namesi
Name:PBD1
Synonyms:PRCGB
Ordered Locus Names:At3g22630
ORF Names:F16J14.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G22630.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. cytosol Source: TAIR
  3. nucleus Source: UniProtKB-SubCell
  4. plasma membrane Source: TAIR
  5. proteasome complex Source: TAIR
  6. proteasome core complex Source: InterPro
  7. vacuolar membrane Source: TAIR
  8. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Proteasome subunit beta type-2-APRO_0000148049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO23714.
PRIDEiO23714.

Expressioni

Gene expression databases

GenevestigatoriO23714.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi7166. 1 interaction.
IntActiO23714. 1 interaction.
STRINGi3702.AT3G22630.1-P.

Structurei

3D structure databases

ProteinModelPortaliO23714.
SMRiO23714. Positions 1-189.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000188743.
InParanoidiO23714.
KOiK02734.
OMAiPRDRHQD.
PhylomeDBiO23714.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23714-1 [UniParc]FASTAAdd to Basket

« Hide

MECVFGLVGN GFAIVAADTS AVHSILLHKN NEDKIMVLDS HKLVAASGEP    50
GDRVQFTEYV QKNVSLYKFR NGIPLTTAAA ANFTRGELAT ALRKNPYSVN 100
ILMAGYDDES GASLYYIDYI ATLHKVDKGA FGYGSYFSLS TMDRHYRSDM 150
SVEEAIELVD KCILEIRSRL VVAPPNFVIK IVDKDGARDY AWRQSVKDVT 200
TAVV 204
Length:204
Mass (Da):22,541
Last modified:January 1, 1998 - v1
Checksum:iB0EE0400FEF1F7AD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13692 mRNA. Translation: CAA74026.1.
AF043534 mRNA. Translation: AAC32070.1.
AP000731 Genomic DNA. Translation: BAB01477.1.
CP002686 Genomic DNA. Translation: AEE76659.1.
AY052249 mRNA. Translation: AAK97719.1.
AY143817 mRNA. Translation: AAN28756.1.
PIRiT51982.
RefSeqiNP_188902.1. NM_113162.2.
UniGeneiAt.398.
At.48695.

Genome annotation databases

EnsemblPlantsiAT3G22630.1; AT3G22630.1; AT3G22630.
GeneIDi821834.
KEGGiath:AT3G22630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13692 mRNA. Translation: CAA74026.1 .
AF043534 mRNA. Translation: AAC32070.1 .
AP000731 Genomic DNA. Translation: BAB01477.1 .
CP002686 Genomic DNA. Translation: AEE76659.1 .
AY052249 mRNA. Translation: AAK97719.1 .
AY143817 mRNA. Translation: AAN28756.1 .
PIRi T51982.
RefSeqi NP_188902.1. NM_113162.2.
UniGenei At.398.
At.48695.

3D structure databases

ProteinModelPortali O23714.
SMRi O23714. Positions 1-189.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 7166. 1 interaction.
IntActi O23714. 1 interaction.
STRINGi 3702.AT3G22630.1-P.

Protein family/group databases

MEROPSi T01.984.

Proteomic databases

PaxDbi O23714.
PRIDEi O23714.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G22630.1 ; AT3G22630.1 ; AT3G22630 .
GeneIDi 821834.
KEGGi ath:AT3G22630.

Organism-specific databases

TAIRi AT3G22630.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000188743.
InParanoidi O23714.
KOi K02734.
OMAi PRDRHQD.
PhylomeDBi O23714.

Enzyme and pathway databases

BioCyci ARA:AT3G22630-MONOMER.

Gene expression databases

Genevestigatori O23714.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 20S proteasome gene family in Arabidopsis thaliana."
    Parmentier Y., Bouchez D., Fleck J., Genschik P.
    FEBS Lett. 416:281-285(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
    Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
    Genetics 149:677-692(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Structure and functional analyses of the 26S proteasome subunits from plants."
    Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D.
    Mol. Biol. Rep. 26:137-146(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  7. "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
    Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
    J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
    Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
    J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT MET-1.

Entry informationi

Entry nameiPSB2A_ARATH
AccessioniPrimary (citable) accession number: O23714
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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