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Protein

Proteasome subunit beta type-2-A

Gene

PBD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciARA:AT3G22630-MONOMER.
ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-2-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit D-1
Proteasome component GB
Proteasome subunit beta type-4
Gene namesi
Name:PBD1
Synonyms:PRCGB
Ordered Locus Names:At3g22630
ORF Names:F16J14.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G22630.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

  • apoplast Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
  • proteasome complex Source: TAIR
  • proteasome core complex Source: InterPro
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Proteasome subunit beta type-2-APRO_0000148049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO23714.
PRIDEiO23714.

Expressioni

Gene expression databases

GenevisibleiO23714. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi7166. 2 interactions.
IntActiO23714. 1 interaction.
STRINGi3702.AT3G22630.1.

Structurei

3D structure databases

ProteinModelPortaliO23714.
SMRiO23714. Positions 1-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0177. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000188743.
InParanoidiO23714.
KOiK02734.
OMAiDRYHDPE.
OrthoDBiEOG09360MSV.
PhylomeDBiO23714.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23714-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MECVFGLVGN GFAIVAADTS AVHSILLHKN NEDKIMVLDS HKLVAASGEP
60 70 80 90 100
GDRVQFTEYV QKNVSLYKFR NGIPLTTAAA ANFTRGELAT ALRKNPYSVN
110 120 130 140 150
ILMAGYDDES GASLYYIDYI ATLHKVDKGA FGYGSYFSLS TMDRHYRSDM
160 170 180 190 200
SVEEAIELVD KCILEIRSRL VVAPPNFVIK IVDKDGARDY AWRQSVKDVT

TAVV
Length:204
Mass (Da):22,541
Last modified:January 1, 1998 - v1
Checksum:iB0EE0400FEF1F7AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13692 mRNA. Translation: CAA74026.1.
AF043534 mRNA. Translation: AAC32070.1.
AP000731 Genomic DNA. Translation: BAB01477.1.
CP002686 Genomic DNA. Translation: AEE76659.1.
AY052249 mRNA. Translation: AAK97719.1.
AY143817 mRNA. Translation: AAN28756.1.
PIRiT51982.
RefSeqiNP_188902.1. NM_113162.2.
UniGeneiAt.398.
At.48695.

Genome annotation databases

EnsemblPlantsiAT3G22630.1; AT3G22630.1; AT3G22630.
GeneIDi821834.
GrameneiAT3G22630.1; AT3G22630.1; AT3G22630.
KEGGiath:AT3G22630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13692 mRNA. Translation: CAA74026.1.
AF043534 mRNA. Translation: AAC32070.1.
AP000731 Genomic DNA. Translation: BAB01477.1.
CP002686 Genomic DNA. Translation: AEE76659.1.
AY052249 mRNA. Translation: AAK97719.1.
AY143817 mRNA. Translation: AAN28756.1.
PIRiT51982.
RefSeqiNP_188902.1. NM_113162.2.
UniGeneiAt.398.
At.48695.

3D structure databases

ProteinModelPortaliO23714.
SMRiO23714. Positions 1-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7166. 2 interactions.
IntActiO23714. 1 interaction.
STRINGi3702.AT3G22630.1.

Protein family/group databases

MEROPSiT01.984.

Proteomic databases

PaxDbiO23714.
PRIDEiO23714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22630.1; AT3G22630.1; AT3G22630.
GeneIDi821834.
GrameneiAT3G22630.1; AT3G22630.1; AT3G22630.
KEGGiath:AT3G22630.

Organism-specific databases

TAIRiAT3G22630.

Phylogenomic databases

eggNOGiKOG0177. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000188743.
InParanoidiO23714.
KOiK02734.
OMAiDRYHDPE.
OrthoDBiEOG09360MSV.
PhylomeDBiO23714.

Enzyme and pathway databases

BioCyciARA:AT3G22630-MONOMER.
ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO23714.

Gene expression databases

GenevisibleiO23714. AT.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB2A_ARATH
AccessioniPrimary (citable) accession number: O23714
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.