O23712 (PSA1B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-1-B
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit F-2
Proteasome component 2B
Proteasome subunit alpha type-6

Gene names

Name:	PAF2
Synonyms:	PRC2B, PRS1
Ordered Locus Names:	At1g47250
ORF Names:	F8G22.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. May play a role in thiol biosynthesis and arsenic tolerance in association with PAF1/ARS5. Ref.8
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.6 Ref.7
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Disruption phenotype	Do not show arsenic tolerance phenotype. Ref.8
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Proteasome subunit alpha type-1-B		PRO_0000124073

Sequences

	Sequence		Length	Mass (Da)
	O23712 [UniParc]. Last modified May 1, 1999. Version 2. Checksum: C5FBCE302217C521	FASTA	277	30,410
	10 20 30 40 50 60 MFRNQYDTDV TTWSPTGRLF QVEYAMEAVK QGSAAIGLRS RSHVVLACVN KAQSELSSHQ 70 80 90 100 110 120 RKIFKVDDHI GVAIAGLTAD GRVLSRYMRS ESINHSFTYE SPLPVGRLVV HLADKAQVCT 130 140 150 160 170 180 QRSWKRPYGV GLLVGGLDES GAHLYYNCPS GNYFEYQAFA IGSRSQAAKT YLERKFESFQ 190 200 210 220 230 240 ESSKEDLIKD AIMAIRETLQ GETLKSSLCT VSVLGVDEPF HFLDQESIQK VIDTFEKVPE 250 260 270 EEEDAGEGEA EPEAAPGAAG TGEQGGSGDQ DVAPMEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana." Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D. Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The 20S proteasome gene family in Arabidopsis thaliana." Parmentier Y., Bouchez D., Fleck J., Genschik P. FEBS Lett. 416:281-285(1997) [PubMed: 9373170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-277. Strain: cv. Columbia.
[6]	"Structure and functional analyses of the 26S proteasome subunits from plants." Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D. Mol. Biol. Rep. 26:137-146(1999) [PubMed: 10363660] [Abstract] Cited for: SUBUNIT.
[7]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis." Sung D.-Y., Kim T.-H., Komives E.A., Mendoza-Cozatl D.G., Schroeder J.I. Plant J. 59:802-812(2009) [PubMed: 19453443] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043527 mRNA. Translation: AAC32063.1. AC079677 Genomic DNA. Translation: AAG52642.1. CP002684 Genomic DNA. Translation: AEE32143.1. AY057649 mRNA. Translation: AAL15280.1. AY141996 mRNA. Translation: AAM98260.1. Y13182 mRNA. Translation: CAA73625.1.
IPI	IPI00530242.
PIR	T51975.
RefSeq	NP_175158.1. NM_103619.4.
UniGene	At.48280. At.72522.
3D structure databases
ProteinModelPortal	O23712.
SMR	O23712. Positions 1-232.
ModBase	Search...
Protein-protein interaction databases
IntAct	O23712. 1 interaction.
STRING	O23712.
Protein family/group databases
MEROPS	T01.976.
Proteomic databases
PRIDE	O23712.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G47250.1; AT1G47250.1; AT1G47250.
GeneID	841128.
GenomeReviews	Gene locus AT1G47250 in contig CT485782_GR.
KEGG	ath:AT1G47250.
NMPDR	fig|3702.1.peg.4184.
Organism-specific databases
TAIR	At1g47250.
Phylogenomic databases
eggNOG	KOG0863.
GeneTree	EPGT00050000005271.
HOGENOM	HBG499923.
InParanoid	O23712.
OMA	ELTFKNT.
PhylomeDB	O23712.
ProtClustDB	CLSN2681914.
Gene expression databases
ArrayExpress	O23712.
Genevestigator	O23712.
GermOnline	AT1G47250. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K02725.
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA1B_ARATH

Accession

Primary (citable) accession number: O23712

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program