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O23710 (PSB7A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-7-A

EC=3.4.25.1
Alternative name(s):
20S proteasome beta subunit B-1
Proteasome component FA
Proteasome component FB
Proteasome subunit beta type-2
Gene names
Name:PBB1
Synonyms:PRCFA, PRCFB
Ordered Locus Names:At3g27430
ORF Names:K1G2.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.7 Ref.8

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O23710-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O23710-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-267: EVLLTKITPLLERVEITEV → GSSHQNHPIAGASRNHRSW
     268-273: Missing.
Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 3737Removed in mature form
PRO_0000042830
Chain38 – 273236Proteasome subunit beta type-7-A
PRO_0000042831

Sites

Active site381Nucleophile By similarity

Natural variations

Alternative sequence249 – 26719EVLLT…EITEV → GSSHQNHPIAGASRNHRSW in isoform 2.
VSP_018144
Alternative sequence268 – 2736Missing in isoform 2.
VSP_018145

Experimental info

Sequence conflict271Q → P in CAA73621. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 4133891315E0915C

FASTA27329,555
        10         20         30         40         50         60 
MSQSTVDVPP KGGFSFDLCK RNDMLTQKGL KAPSFLKTGT TIVGLIFKDG VILGADTRAT 

        70         80         90        100        110        120 
EGPIVADKNC EKIHYMAPNI YCCGAGTAAD TEAVTDMVSS QLRLHRYQTG RDSRVITALT 

       130        140        150        160        170        180 
LLKKHLFSYQ GHVSAALVLG GVDITGPHLH TIYPHGSTDT LPFATMGSGS LAAMSVFEAK 

       190        200        210        220        230        240 
YKEGLTRDEG IKLVAESICS GIFNDLGSGS NVDICVITKG NKEYLRNYME PNPRTYVSSK 

       250        260        270 
GYSFTKKTEV LLTKITPLLE RVEITEVGEA MEE 

« Hide

Isoform 2 [UniParc].

Checksum: E6B193FC30548281
Show »

FASTA26728,812

References

« Hide 'large scale' references
[1]"The 20S proteasome gene family in Arabidopsis thaliana."
Parmentier Y., Bouchez D., Fleck J., Genschik P.
FEBS Lett. 416:281-285(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [MRNA] OF 155-273 (ISOFORM 1).
Strain: cv. Columbia.
[2]"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
Genetics 149:677-692(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]"Structure and functional analyses of the 26S proteasome subunits from plants."
Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D.
Mol. Biol. Rep. 26:137-146(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[8]"Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13174 mRNA. Translation: CAA73617.1.
Y13178 mRNA. Translation: CAA73621.1.
AF043530 mRNA. Translation: AAC32066.1.
AB024028 Genomic DNA. Translation: BAA95719.1.
CP002686 Genomic DNA. Translation: AEE77318.1.
CP002686 Genomic DNA. Translation: AEE77319.1.
BT003339 mRNA. Translation: AAO29958.1.
BT006306 mRNA. Translation: AAP13414.1.
AY086464 mRNA. Translation: AAM63467.1.
PIRT51977.
RefSeqNP_566818.1. NM_113658.2. [O23710-1]
NP_850641.1. NM_180310.1. [O23710-2]
UniGeneAt.24382.

3D structure databases

ProteinModelPortalO23710.
SMRO23710. Positions 40-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid7694. 2 interactions.
IntActO23710. 1 interaction.

Proteomic databases

PaxDbO23710.
PRIDEO23710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G27430.2; AT3G27430.2; AT3G27430. [O23710-1]
GeneID822364.
KEGGath:AT3G27430.

Organism-specific databases

GeneFarm5119.
TAIRAT3G27430.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000182856.
InParanoidO23710.
KOK02739.
OMAGYYIAHR.
PhylomeDBO23710.

Enzyme and pathway databases

BioCycARA:AT3G27430-MONOMER.
ARA:GQT-2215-MONOMER.
ARA:GQT-2216-MONOMER.

Gene expression databases

ArrayExpressO23710.
GenevestigatorO23710.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSB7A_ARATH
AccessionPrimary (citable) accession number: O23710
Secondary accession number(s): O81152, Q3EAZ2, Q9T0L7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names