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O23710

- PSB7A_ARATH

UniProt

O23710 - PSB7A_ARATH

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Protein

Proteasome subunit beta type-7-A

Gene

PBB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciARA:AT3G27430-MONOMER.
ARA:GQT-2215-MONOMER.
ARA:GQT-2216-MONOMER.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_187719. ER-Phagosome pathway.
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_240263. Ubiquitin-dependent degradation of Cyclin D1.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_244054. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_252436. CDK-mediated phosphorylation and removal of Cdc6.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-7-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit B-1
Proteasome component FA
Proteasome component FB
Proteasome subunit beta type-2
Gene namesi
Name:PBB1
Synonyms:PRCFA, PRCFB
Ordered Locus Names:At3g27430
ORF Names:K1G2.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G27430.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome complex Source: TAIR
  4. proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 3737Removed in mature formPRO_0000042830Add
BLAST
Chaini38 – 273236Proteasome subunit beta type-7-APRO_0000042831Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiO23710.
PRIDEiO23710.

Expressioni

Gene expression databases

ExpressionAtlasiO23710. baseline and differential.
GenevestigatoriO23710.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.2 Publications

Protein-protein interaction databases

BioGridi7694. 2 interactions.
IntActiO23710. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO23710.
SMRiO23710. Positions 40-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000182856.
InParanoidiO23710.
KOiK02739.
OMAiGYYIAHR.
PhylomeDBiO23710.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O23710-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSTVDVPP KGGFSFDLCK RNDMLTQKGL KAPSFLKTGT TIVGLIFKDG
60 70 80 90 100
VILGADTRAT EGPIVADKNC EKIHYMAPNI YCCGAGTAAD TEAVTDMVSS
110 120 130 140 150
QLRLHRYQTG RDSRVITALT LLKKHLFSYQ GHVSAALVLG GVDITGPHLH
160 170 180 190 200
TIYPHGSTDT LPFATMGSGS LAAMSVFEAK YKEGLTRDEG IKLVAESICS
210 220 230 240 250
GIFNDLGSGS NVDICVITKG NKEYLRNYME PNPRTYVSSK GYSFTKKTEV
260 270
LLTKITPLLE RVEITEVGEA MEE
Length:273
Mass (Da):29,555
Last modified:November 22, 2005 - v2
Checksum:i4133891315E0915C
GO
Isoform 2 (identifier: O23710-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-267: EVLLTKITPLLERVEITEV → GSSHQNHPIAGASRNHRSW
     268-273: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Show »
Length:267
Mass (Da):28,812
Checksum:iE6B193FC30548281
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271Q → P in CAA73621. (PubMed:9373170)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei249 – 26719EVLLT…EITEV → GSSHQNHPIAGASRNHRSW in isoform 2. CuratedVSP_018144Add
BLAST
Alternative sequencei268 – 2736Missing in isoform 2. CuratedVSP_018145

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13174 mRNA. Translation: CAA73617.1.
Y13178 mRNA. Translation: CAA73621.1.
AF043530 mRNA. Translation: AAC32066.1.
AB024028 Genomic DNA. Translation: BAA95719.1.
CP002686 Genomic DNA. Translation: AEE77318.1.
CP002686 Genomic DNA. Translation: AEE77319.1.
BT003339 mRNA. Translation: AAO29958.1.
BT006306 mRNA. Translation: AAP13414.1.
AY086464 mRNA. Translation: AAM63467.1.
PIRiT51977.
RefSeqiNP_566818.1. NM_113658.2. [O23710-1]
NP_850641.1. NM_180310.1. [O23710-2]
UniGeneiAt.24382.

Genome annotation databases

EnsemblPlantsiAT3G27430.2; AT3G27430.2; AT3G27430. [O23710-1]
GeneIDi822364.
KEGGiath:AT3G27430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13174 mRNA. Translation: CAA73617.1 .
Y13178 mRNA. Translation: CAA73621.1 .
AF043530 mRNA. Translation: AAC32066.1 .
AB024028 Genomic DNA. Translation: BAA95719.1 .
CP002686 Genomic DNA. Translation: AEE77318.1 .
CP002686 Genomic DNA. Translation: AEE77319.1 .
BT003339 mRNA. Translation: AAO29958.1 .
BT006306 mRNA. Translation: AAP13414.1 .
AY086464 mRNA. Translation: AAM63467.1 .
PIRi T51977.
RefSeqi NP_566818.1. NM_113658.2. [O23710-1 ]
NP_850641.1. NM_180310.1. [O23710-2 ]
UniGenei At.24382.

3D structure databases

ProteinModelPortali O23710.
SMRi O23710. Positions 40-258.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 7694. 2 interactions.
IntActi O23710. 1 interaction.

Proteomic databases

PaxDbi O23710.
PRIDEi O23710.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G27430.2 ; AT3G27430.2 ; AT3G27430 . [O23710-1 ]
GeneIDi 822364.
KEGGi ath:AT3G27430.

Organism-specific databases

GeneFarmi 5119.
TAIRi AT3G27430.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000182856.
InParanoidi O23710.
KOi K02739.
OMAi GYYIAHR.
PhylomeDBi O23710.

Enzyme and pathway databases

BioCyci ARA:AT3G27430-MONOMER.
ARA:GQT-2215-MONOMER.
ARA:GQT-2216-MONOMER.
Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_187719. ER-Phagosome pathway.
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_240263. Ubiquitin-dependent degradation of Cyclin D1.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_244054. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_252436. CDK-mediated phosphorylation and removal of Cdc6.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

ExpressionAtlasi O23710. baseline and differential.
Genevestigatori O23710.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 20S proteasome gene family in Arabidopsis thaliana."
    Parmentier Y., Bouchez D., Fleck J., Genschik P.
    FEBS Lett. 416:281-285(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [MRNA] OF 155-273 (ISOFORM 1).
    Strain: cv. Columbia.
  2. "Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
    Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
    Genetics 149:677-692(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "Structure and functional analyses of the 26S proteasome subunits from plants."
    Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D.
    Mol. Biol. Rep. 26:137-146(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
    Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
    J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT.

Entry informationi

Entry nameiPSB7A_ARATH
AccessioniPrimary (citable) accession number: O23710
Secondary accession number(s): O81152, Q3EAZ2, Q9T0L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 26, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3