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Protein

Translocase of chloroplast 33, chloroplastic

Gene

TOC33

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Binds GTP, GDP, XTP, but not ATP. Probably specialized in the import of nuclear encoded photosynthetic preproteins from the cytoplasm to the chloroplast, especially during early development stages.10 Publications

Cofactori

Mg2+3 PublicationsNote: Binds 1 Mg2+ ion by subunit.3 Publications

Kineticsi

  1. KM=290 nM for GDP (at pH 7.4)2 Publications
  2. KM=5.7 µM for GTP (at pH 7.6 and 25 degrees Celsius)2 Publications
  1. Vmax=1040 nmol/min/µg enzyme with GTP as substrate (at pH 7.4)2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Magnesium3 Publications
Metal bindingi68 – 681Magnesium3 Publications
Binding sitei160 – 1601GTP; via amide nitrogenCombined sources1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 516GTPCombined sources1 Publication
Nucleotide bindingi65 – 706GTP1 Publication
Nucleotide bindingi208 – 2092GTPCombined sources1 Publication

GO - Molecular functioni

  • GTPase activity Source: TAIR
  • GTP binding Source: TAIR
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
  • protein homodimerization activity Source: TAIR

GO - Biological processi

  • protein targeting to chloroplast Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Receptor

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G02280-MONOMER.
BRENDAi3.6.5.2. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocase of chloroplast 33, chloroplastic (EC:3.6.5.-)
Short name:
AtToc33
Alternative name(s):
33 kDa chloroplast outer envelope protein
Plastid protein import 1
Gene namesi
Name:TOC33
Synonyms:PPI1
Ordered Locus Names:At1g02280
ORF Names:T7I23.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02280.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei37 – 5317HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast outer membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane

Pathology & Biotechi

Disruption phenotypei

Plants exhibits a pale yellowish phenotype.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 506GGVGKS → RGVGNR: Reduced GTPase activity and impaired interaction with TOC159. 1 Publication
Mutagenesisi130 – 1301R → A: Loss of homidimerization and heterodimerization with TOC159, reduction of GTPase activity. 3 Publications
Mutagenesisi170 – 1701S → A: Normal phosphorylation. 1 Publication
Mutagenesisi175 – 1751S → A: Normal phosphorylation. 1 Publication
Mutagenesisi181 – 1811S → A: Loss of phosphorylation, normal activity. 2 Publications
Mutagenesisi181 – 1811S → D or E: According to PubMed:16412428, supposed to mimic the effects of phosphoserine, but normal activity. 2 Publications
Mutagenesisi181 – 1811S → T: Phosphothreonine instead of phosphoserine. 2 Publications
Mutagenesisi190 – 1901S → A: Normal phosphorylation. 1 Publication
Mutagenesisi200 – 2001S → A: Normal phosphorylation. 1 Publication
Mutagenesisi208 – 2081E → Q: Normal GTPase activity, but weaker nucleotide binding. 1 Publication
Mutagenesisi217 – 2171D → N: Normal GTPase activity. 1 Publication
Mutagenesisi219 – 2191D → N: Normal GTPase activity. 1 Publication
Mutagenesisi220 – 2201E → Q: Normal GTPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 297296Translocase of chloroplast 33, chloroplasticPRO_0000352655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Phosphoserine3 Publications

Post-translational modificationi

Phosphorylated by a kinase present in the outer envelope of chloroplast. When Ser-181 is phosphorylated, the binding to preprotein, GTP and GDP is inhibited, and thus, GTPase activity is repressed.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO23680.
PRIDEiO23680.

PTM databases

iPTMnetiO23680.

Expressioni

Tissue specificityi

Mostly expressed in seedlings and flowers, and, to a lower extent, in roots, stems, and leaves.2 Publications

Developmental stagei

Mostly expressed in photosynthetic tissues undergoing rapid growth. Observed in cotyledons and vascular tissues of hypocotyls of young seedling. In roots, restricted to apical and lateral meristems, and vascular bundles. In stems, mostly detected in the upper part. Expressed in young and middle-aged leaves. In flowers, confined to sepals.2 Publications

Inductioni

Up-regulated by CIA2 in leaves. Induced in light but repressed in darkness.2 Publications

Gene expression databases

GenevisibleiO23680. AT.

Interactioni

Subunit structurei

Homodimer, heterodimer with TOC34 and TOC159, and monomer. The homodimerization and the dimerization with TOC159 require the binding of GTP on Arg-130, and a hypothetical coGAP factor. The dimeric form has a higher GTPase activity than the monomeric form. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). Interacts at least with TOC75-3. Forms large complexes including TOC33, pPORA and OEP161 during pPORA import into plastids at the plastid envelope membrane (PubMed:10998188, PubMed:12473690, PubMed:12951325, PubMed:15773849, PubMed:17337454, PubMed:18400179, PubMed:18541539). Interacts with SP1 (PubMed:23118188).8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-639377,EBI-639377
At1g63890Q8L7N42EBI-639377,EBI-6559199
CPK11Q390164EBI-639377,EBI-979321
CPK4Q388695EBI-639377,EBI-979475

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: TAIR

Protein-protein interaction databases

BioGridi24483. 8 interactions.
IntActiO23680. 8 interactions.
STRINGi3702.AT1G02280.1.

Structurei

Secondary structure

1
297
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 124Combined sources
Helixi16 – 3116Combined sources
Beta strandi36 – 438Combined sources
Helixi49 – 579Combined sources
Beta strandi58 – 603Combined sources
Beta strandi75 – 817Combined sources
Beta strandi84 – 907Combined sources
Beta strandi94 – 963Combined sources
Beta strandi97 – 1004Combined sources
Helixi102 – 11110Combined sources
Turni112 – 1143Combined sources
Beta strandi119 – 1279Combined sources
Helixi133 – 14614Combined sources
Helixi148 – 1536Combined sources
Beta strandi154 – 1596Combined sources
Helixi171 – 19020Combined sources
Helixi191 – 1933Combined sources
Helixi194 – 2007Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi224 – 2285Combined sources
Helixi229 – 24113Combined sources
Beta strandi243 – 2453Combined sources
Beta strandi248 – 2503Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J3EX-ray3.20A2-250[»]
3BB3X-ray2.94A1-251[»]
3BB4X-ray2.85A1-251[»]
3DEFX-ray1.96A1-251[»]
ProteinModelPortaliO23680.
SMRiO23680. Positions 2-250.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO23680.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 259226AIG1-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 684Homodimerization1 Publication
Regioni125 – 1306Homodimerization1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IERB. Eukaryota.
ENOG410ZHTP. LUCA.
HOGENOMiHOG000264764.
InParanoidiO23680.
OMAiFPAATQE.
OrthoDBiEOG09360FPG.
PhylomeDBiO23680.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006703. G_AIG1.
IPR027417. P-loop_NTPase.
IPR005688. Toc34.
[Graphical view]
PfamiPF04548. AIG1. 1 hit.
[Graphical view]
PIRSFiPIRSF038134. Toc34. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00991. 3a0901s02IAP34. 1 hit.
PROSITEiPS51720. G_AIG1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLVREWVG FQQFPAATQE KLIEFFGKLK QKDMNSMTVL VLGKGGVGKS
60 70 80 90 100
STVNSLIGEQ VVRVSPFQAE GLRPVMVSRT MGGFTINIID TPGLVEAGYV
110 120 130 140 150
NHQALELIKG FLVNRTIDVL LYVDRLDVYR VDELDKQVVI AITQTFGKEI
160 170 180 190 200
WCKTLLVLTH AQFSPPDELS YETFSSKRSD SLLKTIRAGS KMRKQEFEDS
210 220 230 240 250
AIAVVYAENS GRCSKNDKDE KALPNGEAWI PNLVKAITDV ATNQRKAIHV
260 270 280 290
DKKMVDGSYS DDKGKKLIPL IIGAQYLIVK MIQGAIRNDI KTSGKPL
Length:297
Mass (Da):32,925
Last modified:January 1, 1998 - v1
Checksum:iE48892E123BA412D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471G → A in AAK68809 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010724 mRNA. Translation: CAC17698.1.
U89959 Genomic DNA. Translation: AAC24375.1.
CP002684 Genomic DNA. Translation: AEE27409.1.
CP002684 Genomic DNA. Translation: AEE27410.1.
AY042869 mRNA. Translation: AAK68809.1.
AY056448 mRNA. Translation: AAL08304.1.
BT025654 mRNA. Translation: ABF74715.1.
RefSeqiNP_001117215.1. NM_001123743.1.
NP_171730.1. NM_100108.4.
UniGeneiAt.10710.
At.70625.

Genome annotation databases

EnsemblPlantsiAT1G02280.1; AT1G02280.1; AT1G02280.
AT1G02280.2; AT1G02280.2; AT1G02280.
GeneIDi839248.
GrameneiAT1G02280.1; AT1G02280.1; AT1G02280.
AT1G02280.2; AT1G02280.2; AT1G02280.
KEGGiath:AT1G02280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010724 mRNA. Translation: CAC17698.1.
U89959 Genomic DNA. Translation: AAC24375.1.
CP002684 Genomic DNA. Translation: AEE27409.1.
CP002684 Genomic DNA. Translation: AEE27410.1.
AY042869 mRNA. Translation: AAK68809.1.
AY056448 mRNA. Translation: AAL08304.1.
BT025654 mRNA. Translation: ABF74715.1.
RefSeqiNP_001117215.1. NM_001123743.1.
NP_171730.1. NM_100108.4.
UniGeneiAt.10710.
At.70625.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J3EX-ray3.20A2-250[»]
3BB3X-ray2.94A1-251[»]
3BB4X-ray2.85A1-251[»]
3DEFX-ray1.96A1-251[»]
ProteinModelPortaliO23680.
SMRiO23680. Positions 2-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24483. 8 interactions.
IntActiO23680. 8 interactions.
STRINGi3702.AT1G02280.1.

PTM databases

iPTMnetiO23680.

Proteomic databases

PaxDbiO23680.
PRIDEiO23680.

Protocols and materials databases

DNASUi839248.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02280.1; AT1G02280.1; AT1G02280.
AT1G02280.2; AT1G02280.2; AT1G02280.
GeneIDi839248.
GrameneiAT1G02280.1; AT1G02280.1; AT1G02280.
AT1G02280.2; AT1G02280.2; AT1G02280.
KEGGiath:AT1G02280.

Organism-specific databases

TAIRiAT1G02280.

Phylogenomic databases

eggNOGiENOG410IERB. Eukaryota.
ENOG410ZHTP. LUCA.
HOGENOMiHOG000264764.
InParanoidiO23680.
OMAiFPAATQE.
OrthoDBiEOG09360FPG.
PhylomeDBiO23680.

Enzyme and pathway databases

BioCyciARA:AT1G02280-MONOMER.
BRENDAi3.6.5.2. 399.

Miscellaneous databases

EvolutionaryTraceiO23680.
PROiO23680.

Gene expression databases

GenevisibleiO23680. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006703. G_AIG1.
IPR027417. P-loop_NTPase.
IPR005688. Toc34.
[Graphical view]
PfamiPF04548. AIG1. 1 hit.
[Graphical view]
PIRSFiPIRSF038134. Toc34. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00991. 3a0901s02IAP34. 1 hit.
PROSITEiPS51720. G_AIG1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOC33_ARATH
AccessioniPrimary (citable) accession number: O23680
Secondary accession number(s): Q94B42, Q9GDD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.