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O23654

- VATA_ARATH

UniProt

O23654 - VATA_ARATH

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Protein

V-type proton ATPase catalytic subunit A

Gene

VHA-A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi252 – 2598ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protease binding Source: UniProtKB
  3. proton-transporting ATPase activity, rotational mechanism Source: InterPro

GO - Biological processi

  1. ATP hydrolysis coupled proton transport Source: InterPro
  2. Golgi organization Source: TAIR
  3. pollen development Source: TAIR
  4. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G78900-MONOMER.
ARA:GQT-1615-MONOMER.
ReactomeiREACT_190851. Phagosomal maturation (early endosomal stage).
REACT_239607. Transferrin endocytosis and recycling.
REACT_253823. Insulin receptor recycling.

Protein family/group databases

TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase catalytic subunit A (EC:3.6.3.14)
Short name:
V-ATPase subunit A
Alternative name(s):
V-ATPase 69 kDa subunit
Vacuolar H(+)-ATPase subunit A
Vacuolar proton pump subunit alpha
Gene namesi
Name:VHA-A
Ordered Locus Names:At1g78900
ORF Names:F9K20.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G78900.

Subcellular locationi

Vacuole membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. cell wall Source: TAIR
  3. chloroplast Source: TAIR
  4. chloroplast envelope Source: TAIR
  5. Golgi apparatus Source: TAIR
  6. membrane Source: TAIR
  7. plant-type vacuole Source: TAIR
  8. plasma membrane Source: TAIR
  9. plasmodesma Source: TAIR
  10. proton-transporting V-type ATPase, V1 domain Source: InterPro
  11. vacuolar membrane Source: TAIR
  12. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623V-type proton ATPase catalytic subunit APRO_0000144574Add
BLAST

Proteomic databases

PaxDbiO23654.
PRIDEiO23654.

Expressioni

Gene expression databases

ExpressionAtlasiO23654. baseline and differential.
GenevestigatoriO23654.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e). Binds to the deubiquitinating enzyme AMSH3.

Protein-protein interaction databases

BioGridi29447. 3 interactions.
IntActiO23654. 4 interactions.
MINTiMINT-8068433.
STRINGi3702.AT1G78900.2-P.

Structurei

3D structure databases

ProteinModelPortaliO23654.
SMRiO23654. Positions 22-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiCOG1155.
HOGENOMiHOG000161057.
InParanoidiO23654.
KOiK02145.
OMAiETTRMIR.
PhylomeDBiO23654.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00309. ATP_synth_A_arch.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR005725. ATPase_V1-cplx_asu.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01042. V-ATPase_V1_A. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23654-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPAFYGGKLT TFEDDEKESE YGYVRKVSGP VVVADGMAGA AMYELVRVGH
60 70 80 90 100
DNLIGEIIRL EGDSATIQVY EETAGLTVND PVLRTHKPLS VELGPGILGN
110 120 130 140 150
IFDGIQRPLK TIARISGDVY IPRGVSVPAL DKDCLWEFQP NKFVEGDTIT
160 170 180 190 200
GGDLYATVFE NTLMNHLVAL PPDAMGKITY IAPAGQYSLK DTVIELEFQG
210 220 230 240 250
IKKSYTMLQS WPVRTPRPVA SKLAADTPLL TGQRVLDALF PSVLGGTCAI
260 270 280 290 300
PGAFGCGKTV ISQALSKYSN SDAVVYVGCG ERGNEMAEVL MDFPQLTMTL
310 320 330 340 350
PDGREESVMK RTTLVANTSN MPVAAREASI YTGITIAEYF RDMGYNVSMM
360 370 380 390 400
ADSTSRWAEA LREISGRLAE MPADSGYPAY LAARLASFYE RAGKVKCLGG
410 420 430 440 450
PERNGSVTIV GAVSPPGGDF SDPVTSATLS IVQVFWGLDK KLAQRKHFPS
460 470 480 490 500
VNWLISYSKY STALESFYEK FDPDFINIRT KAREVLQRED DLNEIVQLVG
510 520 530 540 550
KDALAEGDKI TLETAKLLRE DYLAQNAFTP YDKFCPFYKS VWMMRNIIHF
560 570 580 590 600
YNLANQAVER AAGMDGQKIT YTLIKHRLGD LFYRLVSQKF EDPAEGEDTL
610 620
VEKFKKLYDD LNAGFRALED ETR
Length:623
Mass (Da):68,812
Last modified:January 1, 1998 - v1
Checksum:iF63CAA1A9294CD4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65638 mRNA. Translation: AAB97128.1.
AC005679 Genomic DNA. Translation: AAC83021.1.
CP002684 Genomic DNA. Translation: AEE36171.1.
CP002684 Genomic DNA. Translation: AEE36172.1.
AY059909 mRNA. Translation: AAL24391.1.
AY081296 mRNA. Translation: AAL91185.1.
BT002589 mRNA. Translation: AAO00949.1.
BT008383 mRNA. Translation: AAP37742.1.
AY085759 mRNA. Translation: AAM62977.1.
PIRiE96818.
RefSeqiNP_001031299.1. NM_001036222.2.
NP_178011.1. NM_106539.5.
UniGeneiAt.24629.
At.67925.

Genome annotation databases

EnsemblPlantsiAT1G78900.1; AT1G78900.1; AT1G78900.
AT1G78900.2; AT1G78900.2; AT1G78900.
GeneIDi844228.
KEGGiath:AT1G78900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65638 mRNA. Translation: AAB97128.1 .
AC005679 Genomic DNA. Translation: AAC83021.1 .
CP002684 Genomic DNA. Translation: AEE36171.1 .
CP002684 Genomic DNA. Translation: AEE36172.1 .
AY059909 mRNA. Translation: AAL24391.1 .
AY081296 mRNA. Translation: AAL91185.1 .
BT002589 mRNA. Translation: AAO00949.1 .
BT008383 mRNA. Translation: AAP37742.1 .
AY085759 mRNA. Translation: AAM62977.1 .
PIRi E96818.
RefSeqi NP_001031299.1. NM_001036222.2.
NP_178011.1. NM_106539.5.
UniGenei At.24629.
At.67925.

3D structure databases

ProteinModelPortali O23654.
SMRi O23654. Positions 22-602.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 29447. 3 interactions.
IntActi O23654. 4 interactions.
MINTi MINT-8068433.
STRINGi 3702.AT1G78900.2-P.

Protein family/group databases

TCDBi 3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

PaxDbi O23654.
PRIDEi O23654.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G78900.1 ; AT1G78900.1 ; AT1G78900 .
AT1G78900.2 ; AT1G78900.2 ; AT1G78900 .
GeneIDi 844228.
KEGGi ath:AT1G78900.

Organism-specific databases

TAIRi AT1G78900.

Phylogenomic databases

eggNOGi COG1155.
HOGENOMi HOG000161057.
InParanoidi O23654.
KOi K02145.
OMAi ETTRMIR.
PhylomeDBi O23654.

Enzyme and pathway databases

BioCyci ARA:AT1G78900-MONOMER.
ARA:GQT-1615-MONOMER.
Reactomei REACT_190851. Phagosomal maturation (early endosomal stage).
REACT_239607. Transferrin endocytosis and recycling.
REACT_253823. Insulin receptor recycling.

Miscellaneous databases

PROi O23654.

Gene expression databases

ExpressionAtlasi O23654. baseline and differential.
Genevestigatori O23654.

Family and domain databases

Gene3Di 1.10.1140.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00309. ATP_synth_A_arch.
InterProi IPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR005725. ATPase_V1-cplx_asu.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view ]
Pfami PF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01042. V-ATPase_V1_A. 1 hit.
PROSITEi PS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and isolation of a vacuolar type H+-ATPase subunit a cDNA from Arabidopsis thaliana."
    Magnotta S.M., Gogarten J.P.
    Plant Gene Register PGR97-177
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase."
    Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L.
    Trends Plant Sci. 7:157-161(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana."
    Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T.
    Plant Cell Physiol. 45:672-683(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
  8. "The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana."
    Isono E., Katsiarimpa A., Mueller I.K., Anzenberger F., Stierhof Y.-D., Geldner N., Chory J., Schwechheimer C.
    Plant Cell 22:1826-1837(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AMSH3.

Entry informationi

Entry nameiVATA_ARATH
AccessioniPrimary (citable) accession number: O23654
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3