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O23654

- VATA_ARATH

UniProt

O23654 - VATA_ARATH

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Protein
V-type proton ATPase catalytic subunit A
Gene
VHA-A, At1g78900, F9K20.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.UniRule annotation

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi252 – 2598ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protease binding Source: UniProtKB
  3. proton-transporting ATPase activity, rotational mechanism Source: InterPro

GO - Biological processi

  1. ATP hydrolysis coupled proton transport Source: InterPro
  2. Golgi organization Source: TAIR
  3. pollen development Source: TAIR
  4. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G78900-MONOMER.
ARA:GQT-1615-MONOMER.

Protein family/group databases

TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase catalytic subunit A (EC:3.6.3.14)
Short name:
V-ATPase subunit A
Alternative name(s):
V-ATPase 69 kDa subunit
Vacuolar H(+)-ATPase subunit A
Vacuolar proton pump subunit alpha
Gene namesi
Name:VHA-A
Ordered Locus Names:At1g78900
ORF Names:F9K20.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G78900.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. apoplast Source: TAIR
  3. cell wall Source: TAIR
  4. chloroplast Source: TAIR
  5. chloroplast envelope Source: TAIR
  6. membrane Source: TAIR
  7. plant-type vacuole Source: TAIR
  8. plasma membrane Source: TAIR
  9. plasmodesma Source: TAIR
  10. proton-transporting V-type ATPase, V1 domain Source: InterPro
  11. vacuolar membrane Source: TAIR
  12. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623V-type proton ATPase catalytic subunit AUniRule annotation
PRO_0000144574Add
BLAST

Proteomic databases

PaxDbiO23654.
PRIDEiO23654.

Expressioni

Gene expression databases

GenevestigatoriO23654.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e). Binds to the deubiquitinating enzyme AMSH3.1 Publication

Protein-protein interaction databases

BioGridi29447. 3 interactions.
IntActiO23654. 4 interactions.
MINTiMINT-8068433.
STRINGi3702.AT1G78900.2-P.

Structurei

3D structure databases

ProteinModelPortaliO23654.
SMRiO23654. Positions 22-602.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1155.
HOGENOMiHOG000161057.
InParanoidiO23654.
KOiK02145.
OMAiETTRMIR.
PhylomeDBiO23654.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00309. ATP_synth_A_arch.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR005725. ATPase_V1-cplx_asu.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01042. V-ATPase_V1_A. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23654-1 [UniParc]FASTAAdd to Basket

« Hide

MPAFYGGKLT TFEDDEKESE YGYVRKVSGP VVVADGMAGA AMYELVRVGH    50
DNLIGEIIRL EGDSATIQVY EETAGLTVND PVLRTHKPLS VELGPGILGN 100
IFDGIQRPLK TIARISGDVY IPRGVSVPAL DKDCLWEFQP NKFVEGDTIT 150
GGDLYATVFE NTLMNHLVAL PPDAMGKITY IAPAGQYSLK DTVIELEFQG 200
IKKSYTMLQS WPVRTPRPVA SKLAADTPLL TGQRVLDALF PSVLGGTCAI 250
PGAFGCGKTV ISQALSKYSN SDAVVYVGCG ERGNEMAEVL MDFPQLTMTL 300
PDGREESVMK RTTLVANTSN MPVAAREASI YTGITIAEYF RDMGYNVSMM 350
ADSTSRWAEA LREISGRLAE MPADSGYPAY LAARLASFYE RAGKVKCLGG 400
PERNGSVTIV GAVSPPGGDF SDPVTSATLS IVQVFWGLDK KLAQRKHFPS 450
VNWLISYSKY STALESFYEK FDPDFINIRT KAREVLQRED DLNEIVQLVG 500
KDALAEGDKI TLETAKLLRE DYLAQNAFTP YDKFCPFYKS VWMMRNIIHF 550
YNLANQAVER AAGMDGQKIT YTLIKHRLGD LFYRLVSQKF EDPAEGEDTL 600
VEKFKKLYDD LNAGFRALED ETR 623
Length:623
Mass (Da):68,812
Last modified:January 1, 1998 - v1
Checksum:iF63CAA1A9294CD4F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U65638 mRNA. Translation: AAB97128.1.
AC005679 Genomic DNA. Translation: AAC83021.1.
CP002684 Genomic DNA. Translation: AEE36171.1.
CP002684 Genomic DNA. Translation: AEE36172.1.
AY059909 mRNA. Translation: AAL24391.1.
AY081296 mRNA. Translation: AAL91185.1.
BT002589 mRNA. Translation: AAO00949.1.
BT008383 mRNA. Translation: AAP37742.1.
AY085759 mRNA. Translation: AAM62977.1.
PIRiE96818.
RefSeqiNP_001031299.1. NM_001036222.2.
NP_178011.1. NM_106539.5.
UniGeneiAt.24629.
At.67925.

Genome annotation databases

EnsemblPlantsiAT1G78900.1; AT1G78900.1; AT1G78900.
AT1G78900.2; AT1G78900.2; AT1G78900.
GeneIDi844228.
KEGGiath:AT1G78900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U65638 mRNA. Translation: AAB97128.1 .
AC005679 Genomic DNA. Translation: AAC83021.1 .
CP002684 Genomic DNA. Translation: AEE36171.1 .
CP002684 Genomic DNA. Translation: AEE36172.1 .
AY059909 mRNA. Translation: AAL24391.1 .
AY081296 mRNA. Translation: AAL91185.1 .
BT002589 mRNA. Translation: AAO00949.1 .
BT008383 mRNA. Translation: AAP37742.1 .
AY085759 mRNA. Translation: AAM62977.1 .
PIRi E96818.
RefSeqi NP_001031299.1. NM_001036222.2.
NP_178011.1. NM_106539.5.
UniGenei At.24629.
At.67925.

3D structure databases

ProteinModelPortali O23654.
SMRi O23654. Positions 22-602.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 29447. 3 interactions.
IntActi O23654. 4 interactions.
MINTi MINT-8068433.
STRINGi 3702.AT1G78900.2-P.

Protein family/group databases

TCDBi 3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

PaxDbi O23654.
PRIDEi O23654.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G78900.1 ; AT1G78900.1 ; AT1G78900 .
AT1G78900.2 ; AT1G78900.2 ; AT1G78900 .
GeneIDi 844228.
KEGGi ath:AT1G78900.

Organism-specific databases

TAIRi AT1G78900.

Phylogenomic databases

eggNOGi COG1155.
HOGENOMi HOG000161057.
InParanoidi O23654.
KOi K02145.
OMAi ETTRMIR.
PhylomeDBi O23654.

Enzyme and pathway databases

BioCyci ARA:AT1G78900-MONOMER.
ARA:GQT-1615-MONOMER.

Miscellaneous databases

PROi O23654.

Gene expression databases

Genevestigatori O23654.

Family and domain databases

Gene3Di 1.10.1140.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00309. ATP_synth_A_arch.
InterProi IPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR005725. ATPase_V1-cplx_asu.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view ]
Pfami PF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01042. V-ATPase_V1_A. 1 hit.
PROSITEi PS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and isolation of a vacuolar type H+-ATPase subunit a cDNA from Arabidopsis thaliana."
    Magnotta S.M., Gogarten J.P.
    Plant Gene Register PGR97-177
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase."
    Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L.
    Trends Plant Sci. 7:157-161(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana."
    Isono E., Katsiarimpa A., Mueller I.K., Anzenberger F., Stierhof Y.-D., Geldner N., Chory J., Schwechheimer C.
    Plant Cell 22:1826-1837(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AMSH3.

Entry informationi

Entry nameiVATA_ARATH
AccessioniPrimary (citable) accession number: O23654
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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