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Protein

V-type proton ATPase catalytic subunit A

Gene

VHA-A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi252 – 259ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protease binding Source: UniProtKB
  • proton-transporting ATPase activity, rotational mechanism Source: GO_Central

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: InterPro
  • ATP metabolic process Source: InterPro
  • Golgi organization Source: TAIR
  • pollen development Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G78900-MONOMER
ReactomeiR-ATH-1222556 ROS, RNS production in phagocytes
R-ATH-77387 Insulin receptor recycling
R-ATH-917977 Transferrin endocytosis and recycling

Protein family/group databases

TCDBi3.A.2.2.5 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase catalytic subunit A (EC:3.6.3.14)
Short name:
V-ATPase subunit A
Alternative name(s):
V-ATPase 69 kDa subunit
Vacuolar H(+)-ATPase subunit A
Vacuolar proton pump subunit alpha
Gene namesi
Name:VHA-A
Ordered Locus Names:At1g78900
ORF Names:F9K20.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G78900
TAIRilocus:2037493 AT1G78900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001445741 – 623V-type proton ATPase catalytic subunit AAdd BLAST623

Proteomic databases

PaxDbiO23654
PRIDEiO23654

PTM databases

iPTMnetiO23654

Expressioni

Gene expression databases

ExpressionAtlasiO23654 differential
GenevisibleiO23654 AT

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e). Binds to the deubiquitinating enzyme AMSH3.

GO - Molecular functioni

  • protease binding Source: UniProtKB

Protein-protein interaction databases

BioGridi29447, 7 interactors
IntActiO23654, 4 interactors
MINTiO23654
STRINGi3702.AT1G78900.1

Structurei

3D structure databases

ProteinModelPortaliO23654
SMRiO23654
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiKOG1352 Eukaryota
COG1155 LUCA
HOGENOMiHOG000161057
InParanoidiO23654
KOiK02145
OMAiQQLAKWS
OrthoDBiEOG0936046W
PhylomeDBiO23654

Family and domain databases

Gene3Di1.10.1140.10, 1 hit
2.40.30.20, 1 hit
HAMAPiMF_00309 ATP_synth_A_arch, 1 hit
InterProiView protein in InterPro
IPR031686 ATP-synth_a_Xtn
IPR023366 ATP_synth_asu-like_sf
IPR020003 ATPase_a/bsu_AS
IPR004100 ATPase_F1/V1/A1_a/bsu_N
IPR036121 ATPase_F1/V1/A1_a/bsu_N_sf
IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR024034 ATPase_F1/V1_b/a_C
IPR005725 ATPase_V1-cplx_asu
IPR027417 P-loop_NTPase
IPR022878 V-ATPase_asu
PANTHERiPTHR43607 PTHR43607, 1 hit
PfamiView protein in Pfam
PF00006 ATP-synt_ab, 1 hit
PF02874 ATP-synt_ab_N, 1 hit
PF16886 ATP-synt_ab_Xtn, 1 hit
SUPFAMiSSF50615 SSF50615, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01042 V-ATPase_V1_A, 1 hit
PROSITEiView protein in PROSITE
PS00152 ATPASE_ALPHA_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

O23654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAFYGGKLT TFEDDEKESE YGYVRKVSGP VVVADGMAGA AMYELVRVGH
60 70 80 90 100
DNLIGEIIRL EGDSATIQVY EETAGLTVND PVLRTHKPLS VELGPGILGN
110 120 130 140 150
IFDGIQRPLK TIARISGDVY IPRGVSVPAL DKDCLWEFQP NKFVEGDTIT
160 170 180 190 200
GGDLYATVFE NTLMNHLVAL PPDAMGKITY IAPAGQYSLK DTVIELEFQG
210 220 230 240 250
IKKSYTMLQS WPVRTPRPVA SKLAADTPLL TGQRVLDALF PSVLGGTCAI
260 270 280 290 300
PGAFGCGKTV ISQALSKYSN SDAVVYVGCG ERGNEMAEVL MDFPQLTMTL
310 320 330 340 350
PDGREESVMK RTTLVANTSN MPVAAREASI YTGITIAEYF RDMGYNVSMM
360 370 380 390 400
ADSTSRWAEA LREISGRLAE MPADSGYPAY LAARLASFYE RAGKVKCLGG
410 420 430 440 450
PERNGSVTIV GAVSPPGGDF SDPVTSATLS IVQVFWGLDK KLAQRKHFPS
460 470 480 490 500
VNWLISYSKY STALESFYEK FDPDFINIRT KAREVLQRED DLNEIVQLVG
510 520 530 540 550
KDALAEGDKI TLETAKLLRE DYLAQNAFTP YDKFCPFYKS VWMMRNIIHF
560 570 580 590 600
YNLANQAVER AAGMDGQKIT YTLIKHRLGD LFYRLVSQKF EDPAEGEDTL
610 620
VEKFKKLYDD LNAGFRALED ETR
Length:623
Mass (Da):68,812
Last modified:January 1, 1998 - v1
Checksum:iF63CAA1A9294CD4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65638 mRNA Translation: AAB97128.1
AC005679 Genomic DNA Translation: AAC83021.1
CP002684 Genomic DNA Translation: AEE36171.1
CP002684 Genomic DNA Translation: AEE36172.1
AY059909 mRNA Translation: AAL24391.1
AY081296 mRNA Translation: AAL91185.1
BT002589 mRNA Translation: AAO00949.1
BT008383 mRNA Translation: AAP37742.1
AY085759 mRNA Translation: AAM62977.1
PIRiE96818
RefSeqiNP_001031299.1, NM_001036222.3
NP_178011.1, NM_106539.6
UniGeneiAt.24629
At.67925

Genome annotation databases

EnsemblPlantsiAT1G78900.1; AT1G78900.1; AT1G78900
AT1G78900.2; AT1G78900.2; AT1G78900
GeneIDi844228
GrameneiAT1G78900.1; AT1G78900.1; AT1G78900
AT1G78900.2; AT1G78900.2; AT1G78900
KEGGiath:AT1G78900

Similar proteinsi

Entry informationi

Entry nameiVATA_ARATH
AccessioniPrimary (citable) accession number: O23654
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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