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O23654 (VATA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
V-type proton ATPase catalytic subunit A

Short name=V-ATPase subunit A
EC=3.6.3.14
Alternative name(s):
V-ATPase 69 kDa subunit
Vacuolar H(+)-ATPase subunit A
Vacuolar proton pump subunit alpha
Gene names
Name:VHA-A
Ordered Locus Names:At1g78900
ORF Names:F9K20.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. HAMAP-Rule MF_00309

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out). HAMAP-Rule MF_00309

Subunit structure

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e). Binds to the deubiquitinating enzyme AMSH3. Ref.7

Subcellular location

Vacuole membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00309.

Sequence similarities

Belongs to the ATPase alpha/beta chains family.

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentMembrane
Vacuole
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP hydrolysis coupled proton transport

Inferred from electronic annotation. Source: InterPro

Golgi organization

Inferred from mutant phenotype PubMed 15610354. Source: TAIR

pollen development

Inferred from mutant phenotype PubMed 15610354. Source: TAIR

response to salt stress

Inferred from expression pattern PubMed 17916636. Source: TAIR

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 22430844. Source: TAIR

apoplast

Inferred from direct assay PubMed 21798377. Source: TAIR

cell wall

Inferred from direct assay PubMed 16287169. Source: TAIR

chloroplast

Inferred from direct assay PubMed 15028209PubMed 18431481. Source: TAIR

chloroplast envelope

Inferred from direct assay PubMed 12766230PubMed 12938931. Source: TAIR

membrane

Inferred from direct assay PubMed 17432890. Source: TAIR

plant-type vacuole

Inferred from direct assay PubMed 16618929. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 17317660PubMed 17644812. Source: TAIR

plasmodesma

Inferred from direct assay PubMed 21533090. Source: TAIR

proton-transporting V-type ATPase, V1 domain

Inferred from electronic annotation. Source: InterPro

vacuolar membrane

Inferred from direct assay PubMed 16618929PubMed 17151019. Source: TAIR

vacuole

Inferred from direct assay PubMed 14760709PubMed 15215502PubMed 15539469PubMed 22923678. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protease binding

Inferred from physical interaction Ref.7. Source: UniProtKB

proton-transporting ATPase activity, rotational mechanism

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623V-type proton ATPase catalytic subunit A HAMAP-Rule MF_00309
PRO_0000144574

Regions

Nucleotide binding252 – 2598ATP Potential

Sequences

Sequence LengthMass (Da)Tools
O23654 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: F63CAA1A9294CD4F

FASTA62368,812
        10         20         30         40         50         60 
MPAFYGGKLT TFEDDEKESE YGYVRKVSGP VVVADGMAGA AMYELVRVGH DNLIGEIIRL 

        70         80         90        100        110        120 
EGDSATIQVY EETAGLTVND PVLRTHKPLS VELGPGILGN IFDGIQRPLK TIARISGDVY 

       130        140        150        160        170        180 
IPRGVSVPAL DKDCLWEFQP NKFVEGDTIT GGDLYATVFE NTLMNHLVAL PPDAMGKITY 

       190        200        210        220        230        240 
IAPAGQYSLK DTVIELEFQG IKKSYTMLQS WPVRTPRPVA SKLAADTPLL TGQRVLDALF 

       250        260        270        280        290        300 
PSVLGGTCAI PGAFGCGKTV ISQALSKYSN SDAVVYVGCG ERGNEMAEVL MDFPQLTMTL 

       310        320        330        340        350        360 
PDGREESVMK RTTLVANTSN MPVAAREASI YTGITIAEYF RDMGYNVSMM ADSTSRWAEA 

       370        380        390        400        410        420 
LREISGRLAE MPADSGYPAY LAARLASFYE RAGKVKCLGG PERNGSVTIV GAVSPPGGDF 

       430        440        450        460        470        480 
SDPVTSATLS IVQVFWGLDK KLAQRKHFPS VNWLISYSKY STALESFYEK FDPDFINIRT 

       490        500        510        520        530        540 
KAREVLQRED DLNEIVQLVG KDALAEGDKI TLETAKLLRE DYLAQNAFTP YDKFCPFYKS 

       550        560        570        580        590        600 
VWMMRNIIHF YNLANQAVER AAGMDGQKIT YTLIKHRLGD LFYRLVSQKF EDPAEGEDTL 

       610        620 
VEKFKKLYDD LNAGFRALED ETR 

« Hide

References

« Hide 'large scale' references
[1]"Characterization and isolation of a vacuolar type H+-ATPase subunit a cDNA from Arabidopsis thaliana."
Magnotta S.M., Gogarten J.P.
Plant Gene Register PGR97-177
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase."
Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L.
Trends Plant Sci. 7:157-161(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana."
Isono E., Katsiarimpa A., Mueller I.K., Anzenberger F., Stierhof Y.-D., Geldner N., Chory J., Schwechheimer C.
Plant Cell 22:1826-1837(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AMSH3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U65638 mRNA. Translation: AAB97128.1.
AC005679 Genomic DNA. Translation: AAC83021.1.
CP002684 Genomic DNA. Translation: AEE36171.1.
CP002684 Genomic DNA. Translation: AEE36172.1.
AY059909 mRNA. Translation: AAL24391.1.
AY081296 mRNA. Translation: AAL91185.1.
BT002589 mRNA. Translation: AAO00949.1.
BT008383 mRNA. Translation: AAP37742.1.
AY085759 mRNA. Translation: AAM62977.1.
PIRE96818.
RefSeqNP_001031299.1. NM_001036222.2.
NP_178011.1. NM_106539.5.
UniGeneAt.24629.
At.67925.

3D structure databases

ProteinModelPortalO23654.
SMRO23654. Positions 22-602.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid29447. 3 interactions.
IntActO23654. 4 interactions.
MINTMINT-8068433.
STRING3702.AT1G78900.2-P.

Protein family/group databases

TCDB3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

PaxDbO23654.
PRIDEO23654.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G78900.1; AT1G78900.1; AT1G78900.
AT1G78900.2; AT1G78900.2; AT1G78900.
GeneID844228.
KEGGath:AT1G78900.

Organism-specific databases

TAIRAT1G78900.

Phylogenomic databases

eggNOGCOG1155.
HOGENOMHOG000161057.
InParanoidO23654.
KOK02145.
OMAETTRMIR.
PhylomeDBO23654.

Enzyme and pathway databases

BioCycARA:AT1G78900-MONOMER.
ARA:GQT-1615-MONOMER.

Gene expression databases

GenevestigatorO23654.

Family and domain databases

Gene3D1.10.1140.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPMF_00309. ATP_synth_A_arch.
InterProIPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR005725. ATPase_V1-cplx_asu.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
SUPFAMSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01042. V-ATPase_V1_A. 1 hit.
PROSITEPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO23654.

Entry information

Entry nameVATA_ARATH
AccessionPrimary (citable) accession number: O23654
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names