Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O23654

- VATA_ARATH

UniProt

O23654 - VATA_ARATH

Protein

V-type proton ATPase catalytic subunit A

Gene

VHA-A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

    Catalytic activityi

    ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi252 – 2598ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protease binding Source: UniProtKB
    3. proton-transporting ATPase activity, rotational mechanism Source: InterPro

    GO - Biological processi

    1. ATP hydrolysis coupled proton transport Source: InterPro
    2. Golgi organization Source: TAIR
    3. pollen development Source: TAIR
    4. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G78900-MONOMER.
    ARA:GQT-1615-MONOMER.

    Protein family/group databases

    TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    V-type proton ATPase catalytic subunit A (EC:3.6.3.14)
    Short name:
    V-ATPase subunit A
    Alternative name(s):
    V-ATPase 69 kDa subunit
    Vacuolar H(+)-ATPase subunit A
    Vacuolar proton pump subunit alpha
    Gene namesi
    Name:VHA-A
    Ordered Locus Names:At1g78900
    ORF Names:F9K20.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G78900.

    Subcellular locationi

    Vacuole membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. cell wall Source: TAIR
    3. chloroplast Source: TAIR
    4. chloroplast envelope Source: TAIR
    5. Golgi apparatus Source: TAIR
    6. membrane Source: TAIR
    7. plant-type vacuole Source: TAIR
    8. plasma membrane Source: TAIR
    9. plasmodesma Source: TAIR
    10. proton-transporting V-type ATPase, V1 domain Source: InterPro
    11. vacuolar membrane Source: TAIR
    12. vacuole Source: TAIR

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 623623V-type proton ATPase catalytic subunit APRO_0000144574Add
    BLAST

    Proteomic databases

    PaxDbiO23654.
    PRIDEiO23654.

    Expressioni

    Gene expression databases

    GenevestigatoriO23654.

    Interactioni

    Subunit structurei

    V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e). Binds to the deubiquitinating enzyme AMSH3.

    Protein-protein interaction databases

    BioGridi29447. 3 interactions.
    IntActiO23654. 4 interactions.
    MINTiMINT-8068433.
    STRINGi3702.AT1G78900.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO23654.
    SMRiO23654. Positions 22-602.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATPase alpha/beta chains family.Curated

    Phylogenomic databases

    eggNOGiCOG1155.
    HOGENOMiHOG000161057.
    InParanoidiO23654.
    KOiK02145.
    OMAiETTRMIR.
    PhylomeDBiO23654.

    Family and domain databases

    Gene3Di1.10.1140.10. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00309. ATP_synth_A_arch.
    InterProiIPR020003. ATPase_a/bsu_AS.
    IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR004100. ATPase_F1_a/bsu_N.
    IPR024034. ATPase_F1_bsu/V1_C.
    IPR005725. ATPase_V1-cplx_asu.
    IPR027417. P-loop_NTPase.
    IPR022878. V-ATPase_asu.
    [Graphical view]
    PfamiPF00006. ATP-synt_ab. 1 hit.
    PF00306. ATP-synt_ab_C. 1 hit.
    PF02874. ATP-synt_ab_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47917. SSF47917. 1 hit.
    SSF50615. SSF50615. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01042. V-ATPase_V1_A. 1 hit.
    PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O23654-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAFYGGKLT TFEDDEKESE YGYVRKVSGP VVVADGMAGA AMYELVRVGH    50
    DNLIGEIIRL EGDSATIQVY EETAGLTVND PVLRTHKPLS VELGPGILGN 100
    IFDGIQRPLK TIARISGDVY IPRGVSVPAL DKDCLWEFQP NKFVEGDTIT 150
    GGDLYATVFE NTLMNHLVAL PPDAMGKITY IAPAGQYSLK DTVIELEFQG 200
    IKKSYTMLQS WPVRTPRPVA SKLAADTPLL TGQRVLDALF PSVLGGTCAI 250
    PGAFGCGKTV ISQALSKYSN SDAVVYVGCG ERGNEMAEVL MDFPQLTMTL 300
    PDGREESVMK RTTLVANTSN MPVAAREASI YTGITIAEYF RDMGYNVSMM 350
    ADSTSRWAEA LREISGRLAE MPADSGYPAY LAARLASFYE RAGKVKCLGG 400
    PERNGSVTIV GAVSPPGGDF SDPVTSATLS IVQVFWGLDK KLAQRKHFPS 450
    VNWLISYSKY STALESFYEK FDPDFINIRT KAREVLQRED DLNEIVQLVG 500
    KDALAEGDKI TLETAKLLRE DYLAQNAFTP YDKFCPFYKS VWMMRNIIHF 550
    YNLANQAVER AAGMDGQKIT YTLIKHRLGD LFYRLVSQKF EDPAEGEDTL 600
    VEKFKKLYDD LNAGFRALED ETR 623
    Length:623
    Mass (Da):68,812
    Last modified:January 1, 1998 - v1
    Checksum:iF63CAA1A9294CD4F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65638 mRNA. Translation: AAB97128.1.
    AC005679 Genomic DNA. Translation: AAC83021.1.
    CP002684 Genomic DNA. Translation: AEE36171.1.
    CP002684 Genomic DNA. Translation: AEE36172.1.
    AY059909 mRNA. Translation: AAL24391.1.
    AY081296 mRNA. Translation: AAL91185.1.
    BT002589 mRNA. Translation: AAO00949.1.
    BT008383 mRNA. Translation: AAP37742.1.
    AY085759 mRNA. Translation: AAM62977.1.
    PIRiE96818.
    RefSeqiNP_001031299.1. NM_001036222.2.
    NP_178011.1. NM_106539.5.
    UniGeneiAt.24629.
    At.67925.

    Genome annotation databases

    EnsemblPlantsiAT1G78900.1; AT1G78900.1; AT1G78900.
    AT1G78900.2; AT1G78900.2; AT1G78900.
    GeneIDi844228.
    KEGGiath:AT1G78900.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65638 mRNA. Translation: AAB97128.1 .
    AC005679 Genomic DNA. Translation: AAC83021.1 .
    CP002684 Genomic DNA. Translation: AEE36171.1 .
    CP002684 Genomic DNA. Translation: AEE36172.1 .
    AY059909 mRNA. Translation: AAL24391.1 .
    AY081296 mRNA. Translation: AAL91185.1 .
    BT002589 mRNA. Translation: AAO00949.1 .
    BT008383 mRNA. Translation: AAP37742.1 .
    AY085759 mRNA. Translation: AAM62977.1 .
    PIRi E96818.
    RefSeqi NP_001031299.1. NM_001036222.2.
    NP_178011.1. NM_106539.5.
    UniGenei At.24629.
    At.67925.

    3D structure databases

    ProteinModelPortali O23654.
    SMRi O23654. Positions 22-602.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29447. 3 interactions.
    IntActi O23654. 4 interactions.
    MINTi MINT-8068433.
    STRINGi 3702.AT1G78900.2-P.

    Protein family/group databases

    TCDBi 3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Proteomic databases

    PaxDbi O23654.
    PRIDEi O23654.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G78900.1 ; AT1G78900.1 ; AT1G78900 .
    AT1G78900.2 ; AT1G78900.2 ; AT1G78900 .
    GeneIDi 844228.
    KEGGi ath:AT1G78900.

    Organism-specific databases

    TAIRi AT1G78900.

    Phylogenomic databases

    eggNOGi COG1155.
    HOGENOMi HOG000161057.
    InParanoidi O23654.
    KOi K02145.
    OMAi ETTRMIR.
    PhylomeDBi O23654.

    Enzyme and pathway databases

    BioCyci ARA:AT1G78900-MONOMER.
    ARA:GQT-1615-MONOMER.

    Miscellaneous databases

    PROi O23654.

    Gene expression databases

    Genevestigatori O23654.

    Family and domain databases

    Gene3Di 1.10.1140.10. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00309. ATP_synth_A_arch.
    InterProi IPR020003. ATPase_a/bsu_AS.
    IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR004100. ATPase_F1_a/bsu_N.
    IPR024034. ATPase_F1_bsu/V1_C.
    IPR005725. ATPase_V1-cplx_asu.
    IPR027417. P-loop_NTPase.
    IPR022878. V-ATPase_asu.
    [Graphical view ]
    Pfami PF00006. ATP-synt_ab. 1 hit.
    PF00306. ATP-synt_ab_C. 1 hit.
    PF02874. ATP-synt_ab_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47917. SSF47917. 1 hit.
    SSF50615. SSF50615. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01042. V-ATPase_V1_A. 1 hit.
    PROSITEi PS00152. ATPASE_ALPHA_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and isolation of a vacuolar type H+-ATPase subunit a cDNA from Arabidopsis thaliana."
      Magnotta S.M., Gogarten J.P.
      Plant Gene Register PGR97-177
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase."
      Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L.
      Trends Plant Sci. 7:157-161(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana."
      Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T.
      Plant Cell Physiol. 45:672-683(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
    8. "The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana."
      Isono E., Katsiarimpa A., Mueller I.K., Anzenberger F., Stierhof Y.-D., Geldner N., Chory J., Schwechheimer C.
      Plant Cell 22:1826-1837(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AMSH3.

    Entry informationi

    Entry nameiVATA_ARATH
    AccessioniPrimary (citable) accession number: O23654
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3