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Protein

Aspartokinase 2, chloroplastic

Gene

AK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Enzyme regulationi

Allosterically inhibited by lysine, but not by S-adenosyl-L-methionine (SAM). K(0.5) for lysine in the presence of physiological concentrations of substrates is 12.5 µM. No inhibition by threonine or leucine and no activation or inhibition by alanine, cysteine, isoleucine, serine, valine, methionine, glutamine, asparagine, glutamic acid or arginine.1 Publication

Kineticsi

K(cat) is 14.5/sec.

  1. KM=980 µM for ATP1 Publication
  2. KM=1940 µM for aspartate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871ATPBy similarity
Binding sitei90 – 901ATP; via amide nitrogenBy similarity
Binding sitei119 – 1191ATPBy similarity
Binding sitei203 – 2031SubstrateBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. aspartate kinase activity Source: UniProtKB-EC
  3. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
  2. threonine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G14060-MONOMER.
ARA:GQT-2418-MONOMER.
SABIO-RKO23653.
UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartokinase 2, chloroplastic (EC:2.7.2.4)
Alternative name(s):
Aspartate kinase 2
Gene namesi
Name:AK2
Synonyms:AK-LYS2, CARAB-AK-LYS
Ordered Locus Names:At5g14060
ORF Names:MUA22.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G14060.

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8484ChloroplastSequence AnalysisAdd
BLAST
Chaini85 – 544460Aspartokinase 2, chloroplasticPRO_0000248158Add
BLAST

Proteomic databases

PaxDbiO23653.
PRIDEiO23653.

Expressioni

Tissue specificityi

Expressed in stems, leaves, floral organs and young seedlings.1 Publication

Gene expression databases

GenevestigatoriO23653.

Structurei

3D structure databases

ProteinModelPortaliO23653.
SMRiO23653. Positions 80-542.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini401 – 47979ACT 1PROSITE-ProRule annotationAdd
BLAST
Domaini481 – 54464ACT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the aspartokinase family.Curated
Contains 2 ACT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG0527.
HOGENOMiHOG000293094.
InParanoidiO23653.
KOiK00928.
OMAiHVEWIND.
PhylomeDBiO23653.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23653-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLQLYGVK TPGLALSSKR LEFASKGACF SVTLPSSSAV FRDVEHSCRN
60 70 80 90 100
IGLRVSCEAL RVDLLQRKEP ETCDSSGTGK ELTCVMKFGG SSVESAERMK
110 120 130 140 150
EVANLILSFP DERPVIVLSA MGKTTNKLLK AGEKAVTCGV TNVESIEELS
160 170 180 190 200
FIKELHLRTA HELGVETTVI EKHLEGLHQL LKGISMMKEL TLRTRDYLVS
210 220 230 240 250
FGECMSTRLF SAYLNKIGHK ARQYDAFEIG FITTDDFTNA DILEATYPAV
260 270 280 290 300
SKTLVGDWSK ENAVPVVTGY LGKGWRSCAI TTLGRGGSDL TATTIGKALG
310 320 330 340 350
LREIQVWKDV DGVLTCDPNI YPGAQSVPYL TFDEAAELAY FGAQVLHPLS
360 370 380 390 400
MRPARDGDIP VRVKNSYNPT APGTVITRSR DMSKAVLTSI VLKRNVTMLD
410 420 430 440 450
IASTRMLGQY GFLAKVFTTF EDLGISVDVV ATSEVSISLT LDPAKLWGRE
460 470 480 490 500
LIQRVNELDN LVEELEKIAV VKLLQRRSII SLIGNVQKSS LILEKVFQVF
510 520 530 540
RSNGVNVQMI SQGASKVNIS LIVNDEEAEQ CVRALHSAFF ETDP
Length:544
Mass (Da):59,605
Last modified:September 5, 2006 - v2
Checksum:i7DBCFDC1138645AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941E → G in AAB63104 (PubMed:9207844).Curated
Sequence conflicti94 – 941E → G in CAC06395 (Ref. 2) Curated
Sequence conflicti107 – 1071L → F in AAB63104 (PubMed:9207844).Curated
Sequence conflicti107 – 1071L → F in CAC06395 (Ref. 2) Curated
Sequence conflicti125 – 1251T → I in AAB63104 (PubMed:9207844).Curated
Sequence conflicti125 – 1251T → I in CAC06395 (Ref. 2) Curated
Sequence conflicti144 – 1441E → G in AAB63104 (PubMed:9207844).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62020 mRNA. Translation: AAB63104.1.
Y16255 Genomic DNA. Translation: CAC06395.1.
AB007650 Genomic DNA. Translation: BAB08285.1.
CP002688 Genomic DNA. Translation: AED91982.1.
CP002688 Genomic DNA. Translation: AED91983.1.
RefSeqiNP_001078581.1. NM_001085112.1.
NP_196910.1. NM_121409.3.
UniGeneiAt.21761.

Genome annotation databases

EnsemblPlantsiAT5G14060.1; AT5G14060.1; AT5G14060.
AT5G14060.2; AT5G14060.2; AT5G14060.
GeneIDi831255.
KEGGiath:AT5G14060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62020 mRNA. Translation: AAB63104.1.
Y16255 Genomic DNA. Translation: CAC06395.1.
AB007650 Genomic DNA. Translation: BAB08285.1.
CP002688 Genomic DNA. Translation: AED91982.1.
CP002688 Genomic DNA. Translation: AED91983.1.
RefSeqiNP_001078581.1. NM_001085112.1.
NP_196910.1. NM_121409.3.
UniGeneiAt.21761.

3D structure databases

ProteinModelPortaliO23653.
SMRiO23653. Positions 80-542.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiO23653.
PRIDEiO23653.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G14060.1; AT5G14060.1; AT5G14060.
AT5G14060.2; AT5G14060.2; AT5G14060.
GeneIDi831255.
KEGGiath:AT5G14060.

Organism-specific databases

TAIRiAT5G14060.

Phylogenomic databases

eggNOGiCOG0527.
HOGENOMiHOG000293094.
InParanoidiO23653.
KOiK00928.
OMAiHVEWIND.
PhylomeDBiO23653.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.
BioCyciARA:AT5G14060-MONOMER.
ARA:GQT-2418-MONOMER.
SABIO-RKO23653.

Gene expression databases

GenevestigatoriO23653.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of an Arabidopsis thaliana cDNA encoding a monofunctional aspartate kinase homologous to the lysine-sensitive enzyme of Escherichia coli."
    Tang G., Zhu-Shimoni J.X., Amir R., Zchori I.B.-T., Galili G.
    Plant Mol. Biol. 34:287-293(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. Frankard V.M.S., Vauterin M., Jacobs M.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Allosteric monofunctional aspartate kinases from Arabidopsis."
    Curien G., Laurencin M., Robert-Genthon M., Dumas R.
    FEBS J. 274:164-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.

Entry informationi

Entry nameiAK2_ARATH
AccessioniPrimary (citable) accession number: O23653
Secondary accession number(s): Q9FMU4, Q9FY44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: January 7, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.