Reviewed,
UniProtKB/Swiss-Prot O23653 (AK2_ARATH)
Last modified
November 3, 2009.
Version 58.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Aspartokinase 2, chloroplastic EC=2.7.2.4 Alternative name(s): Aspartate kinase 2 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 544 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway. |
| Catalytic activity | ATP + L-aspartate = ADP + 4-phospho-L-aspartate. |
| Enzyme regulation | Allosterically inhibited by lysine, but not by S-adenosyl-L-methionine (SAM). K(0.5) for lysine in the presence of physiological concentrations of substrates is 12.5 µM. No inhibition by threonine or leucine and no activation or inhibition by alanine, cysteine, isoleucine, serine, valine, methionine, glutamine, asparagine, glutamic acid or arginine. Ref.4 |
| Pathway | Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. |
| Subcellular location | Plastid › chloroplast Potential. |
| Tissue specificity | Expressed in stems, leaves, floral organs and young seedlings. Ref.1 |
| Sequence similarities | Belongs to the aspartokinase family. Contains 2 ACT domains. |
| Biophysicochemical properties | Kinetic parameters: K(cat) is 14.5/sec. KM=980 µM for ATP KM=1940 µM for aspartate |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Threonine biosynthesis |
| Cellular component | Chloroplast Plastid |
| Domain | Repeat Transit peptide |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid bindingInferred from electronic annotation. Source: InterPro aspartate kinase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 84 | 84 | Chloroplast Potential | ||||||
| Chain | 85 – 544 | 460 | Aspartokinase 2, chloroplastic | PRO_0000248158 | |||||
Regions | |||||||||
| Domain | 402 – 477 | 76 | ACT 1 | ||||||
| Domain | 478 – 539 | 62 | ACT 2 | ||||||
Sites | |||||||||
| Binding site | 87 | 1 | ATP By similarity | ||||||
| Binding site | 90 | 1 | ATP; via amide nitrogen By similarity | ||||||
| Binding site | 119 | 1 | ATP By similarity | ||||||
| Binding site | 203 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 94 | 1 | E → G in AAB63104. Ref.1 | ||||||
| Sequence conflict | 94 | 1 | E → G in CAC06395. Ref.2 | ||||||
| Sequence conflict | 107 | 1 | L → F in AAB63104. Ref.1 | ||||||
| Sequence conflict | 107 | 1 | L → F in CAC06395. Ref.2 | ||||||
| Sequence conflict | 125 | 1 | T → I in AAB63104. Ref.1 | ||||||
| Sequence conflict | 125 | 1 | T → I in CAC06395. Ref.2 | ||||||
| Sequence conflict | 144 | 1 | E → G in AAB63104. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and expression of an Arabidopsis thaliana cDNA encoding a monofunctional aspartate kinase homologous to the lysine-sensitive enzyme of Escherichia coli." Tang G., Zhu-Shimoni J.X., Amir R., Zchori I.B.-T., Galili G. Plant Mol. Biol. 34:287-293(1997) [PubMed: 9207844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. |
| [2] | Frankard V.M.S., Vauterin M., Jacobs M. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Leaf. |
| [3] | "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Allosteric monofunctional aspartate kinases from Arabidopsis." Curien G., Laurencin M., Robert-Genthon M., Dumas R. FEBS J. 274:164-176(2007) [PubMed: 17140415] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U62020 mRNA. Translation: AAB63104.1. Y16255 Genomic DNA. Translation: CAC06395.1. AB007650 Genomic DNA. Translation: BAB08285.1. | |
| IPI | IPI00527711. |
| RefSeq | NP_001078581.1. NP_196910.1. |
| UniGene | At.21761 |
3D structure databases | |
| SMR | O23653. Positions 80-542. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O23653. |
Proteomic databases | |
| PRIDE | O23653. |
Genome annotation databases | |
| GeneID | 831255. |
| GenomeReviews | Gene locus AT5G14060 in contig BA000015_GR. |
| KEGG | ath:AT5G14060. |
| NMPDR | fig|3702.1.peg.23535. |
Organism-specific databases | |
| TAIR | At5g14060. |
Phylogenomic databases | |
| OMA | KLSCLFM. |
Enzyme and pathway databases | |
| BRENDA | 2.7.2.4. 302. |
Gene expression databases | |
| Genevestigator | O23653. |
| GermOnline | AT5G14060. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. [Graphical view] |
| Gene3D | G3DSA:3.40.1160.10. Aa_kinase. 1 hit. |
| Pfam | PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view] |
| TIGRFAMs | TIGR00657. asp_kinases. 1 hit. |
| PROSITE | PS00324. ASPARTOKINASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AK2_ARATH | ||||||||
| Accession | Primary (citable) accession number: O23653 Secondary accession number(s): Q9FMU4, Q9FY44 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


