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O23653

- AK2_ARATH

UniProt

O23653 - AK2_ARATH

Protein

Aspartokinase 2, chloroplastic

Gene

AK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.

    Catalytic activityi

    ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

    Enzyme regulationi

    Allosterically inhibited by lysine, but not by S-adenosyl-L-methionine (SAM). K(0.5) for lysine in the presence of physiological concentrations of substrates is 12.5 µM. No inhibition by threonine or leucine and no activation or inhibition by alanine, cysteine, isoleucine, serine, valine, methionine, glutamine, asparagine, glutamic acid or arginine.1 Publication

    Kineticsi

    K(cat) is 14.5/sec.

    1. KM=980 µM for ATP1 Publication
    2. KM=1940 µM for aspartate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871ATPBy similarity
    Binding sitei90 – 901ATP; via amide nitrogenBy similarity
    Binding sitei119 – 1191ATPBy similarity
    Binding sitei203 – 2031SubstrateBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. aspartate kinase activity Source: UniProtKB-EC
    3. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
    2. threonine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Threonine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G14060-MONOMER.
    ARA:GQT-2418-MONOMER.
    SABIO-RKO23653.
    UniPathwayiUPA00034; UER00015.
    UPA00050; UER00461.
    UPA00051; UER00462.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartokinase 2, chloroplastic (EC:2.7.2.4)
    Alternative name(s):
    Aspartate kinase 2
    Gene namesi
    Name:AK2
    Synonyms:AK-LYS2, CARAB-AK-LYS
    Ordered Locus Names:At5g14060
    ORF Names:MUA22.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G14060.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8484ChloroplastSequence AnalysisAdd
    BLAST
    Chaini85 – 544460Aspartokinase 2, chloroplasticPRO_0000248158Add
    BLAST

    Proteomic databases

    PaxDbiO23653.
    PRIDEiO23653.

    Expressioni

    Tissue specificityi

    Expressed in stems, leaves, floral organs and young seedlings.1 Publication

    Gene expression databases

    GenevestigatoriO23653.

    Structurei

    3D structure databases

    ProteinModelPortaliO23653.
    SMRiO23653. Positions 80-542.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini401 – 47979ACT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini481 – 54464ACT 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the aspartokinase family.Curated
    Contains 2 ACT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0527.
    HOGENOMiHOG000293094.
    InParanoidiO23653.
    KOiK00928.
    OMAiFVLNTWH.
    PhylomeDBiO23653.

    Family and domain databases

    Gene3Di3.40.1160.10. 2 hits.
    InterProiIPR002912. ACT_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001341. Asp_kinase_dom.
    IPR018042. Aspartate_kinase_CS.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01842. ACT. 2 hits.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    PS00324. ASPARTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O23653-1 [UniParc]FASTAAdd to Basket

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    MASLQLYGVK TPGLALSSKR LEFASKGACF SVTLPSSSAV FRDVEHSCRN    50
    IGLRVSCEAL RVDLLQRKEP ETCDSSGTGK ELTCVMKFGG SSVESAERMK 100
    EVANLILSFP DERPVIVLSA MGKTTNKLLK AGEKAVTCGV TNVESIEELS 150
    FIKELHLRTA HELGVETTVI EKHLEGLHQL LKGISMMKEL TLRTRDYLVS 200
    FGECMSTRLF SAYLNKIGHK ARQYDAFEIG FITTDDFTNA DILEATYPAV 250
    SKTLVGDWSK ENAVPVVTGY LGKGWRSCAI TTLGRGGSDL TATTIGKALG 300
    LREIQVWKDV DGVLTCDPNI YPGAQSVPYL TFDEAAELAY FGAQVLHPLS 350
    MRPARDGDIP VRVKNSYNPT APGTVITRSR DMSKAVLTSI VLKRNVTMLD 400
    IASTRMLGQY GFLAKVFTTF EDLGISVDVV ATSEVSISLT LDPAKLWGRE 450
    LIQRVNELDN LVEELEKIAV VKLLQRRSII SLIGNVQKSS LILEKVFQVF 500
    RSNGVNVQMI SQGASKVNIS LIVNDEEAEQ CVRALHSAFF ETDP 544
    Length:544
    Mass (Da):59,605
    Last modified:September 5, 2006 - v2
    Checksum:i7DBCFDC1138645AC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti94 – 941E → G in AAB63104. (PubMed:9207844)Curated
    Sequence conflicti94 – 941E → G in CAC06395. 1 PublicationCurated
    Sequence conflicti107 – 1071L → F in AAB63104. (PubMed:9207844)Curated
    Sequence conflicti107 – 1071L → F in CAC06395. 1 PublicationCurated
    Sequence conflicti125 – 1251T → I in AAB63104. (PubMed:9207844)Curated
    Sequence conflicti125 – 1251T → I in CAC06395. 1 PublicationCurated
    Sequence conflicti144 – 1441E → G in AAB63104. (PubMed:9207844)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62020 mRNA. Translation: AAB63104.1.
    Y16255 Genomic DNA. Translation: CAC06395.1.
    AB007650 Genomic DNA. Translation: BAB08285.1.
    CP002688 Genomic DNA. Translation: AED91982.1.
    CP002688 Genomic DNA. Translation: AED91983.1.
    RefSeqiNP_001078581.1. NM_001085112.1.
    NP_196910.1. NM_121409.3.
    UniGeneiAt.21761.

    Genome annotation databases

    EnsemblPlantsiAT5G14060.1; AT5G14060.1; AT5G14060.
    AT5G14060.2; AT5G14060.2; AT5G14060.
    GeneIDi831255.
    KEGGiath:AT5G14060.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62020 mRNA. Translation: AAB63104.1 .
    Y16255 Genomic DNA. Translation: CAC06395.1 .
    AB007650 Genomic DNA. Translation: BAB08285.1 .
    CP002688 Genomic DNA. Translation: AED91982.1 .
    CP002688 Genomic DNA. Translation: AED91983.1 .
    RefSeqi NP_001078581.1. NM_001085112.1.
    NP_196910.1. NM_121409.3.
    UniGenei At.21761.

    3D structure databases

    ProteinModelPortali O23653.
    SMRi O23653. Positions 80-542.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi O23653.
    PRIDEi O23653.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G14060.1 ; AT5G14060.1 ; AT5G14060 .
    AT5G14060.2 ; AT5G14060.2 ; AT5G14060 .
    GeneIDi 831255.
    KEGGi ath:AT5G14060.

    Organism-specific databases

    TAIRi AT5G14060.

    Phylogenomic databases

    eggNOGi COG0527.
    HOGENOMi HOG000293094.
    InParanoidi O23653.
    KOi K00928.
    OMAi FVLNTWH.
    PhylomeDBi O23653.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00015 .
    UPA00050 ; UER00461 .
    UPA00051 ; UER00462 .
    BioCyci ARA:AT5G14060-MONOMER.
    ARA:GQT-2418-MONOMER.
    SABIO-RK O23653.

    Gene expression databases

    Genevestigatori O23653.

    Family and domain databases

    Gene3Di 3.40.1160.10. 2 hits.
    InterProi IPR002912. ACT_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001341. Asp_kinase_dom.
    IPR018042. Aspartate_kinase_CS.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01842. ACT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    TIGRFAMsi TIGR00657. asp_kinases. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    PS00324. ASPARTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of an Arabidopsis thaliana cDNA encoding a monofunctional aspartate kinase homologous to the lysine-sensitive enzyme of Escherichia coli."
      Tang G., Zhu-Shimoni J.X., Amir R., Zchori I.B.-T., Galili G.
      Plant Mol. Biol. 34:287-293(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. Frankard V.M.S., Vauterin M., Jacobs M.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Allosteric monofunctional aspartate kinases from Arabidopsis."
      Curien G., Laurencin M., Robert-Genthon M., Dumas R.
      FEBS J. 274:164-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.

    Entry informationi

    Entry nameiAK2_ARATH
    AccessioniPrimary (citable) accession number: O23653
    Secondary accession number(s): Q9FMU4, Q9FY44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3