Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic

Gene

SBE2.1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei486NucleophileBy similarity1
Active sitei541Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G36390-MONOMER
UniPathwayiUPA00152

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic (EC:2.4.1.18)
Short name:
AtSBE II-1
Alternative name(s):
Branching enzyme 3
Short name:
AtBE3
Starch-branching enzyme 2-1
Gene namesi
Name:SBE2.1
Synonyms:BE3
Ordered Locus Names:At2g36390
ORF Names:F1O11.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36390
TAIRilocus:2044903 AT2G36390

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Modified starch composition. This phenotype is enhanced when associated with SBE2.2 and SBE3 disruptions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37ChloroplastSequence analysisAdd BLAST37
ChainiPRO_000041533538 – 8581,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplasticAdd BLAST821

Proteomic databases

PaxDbiO23647
PRIDEiO23647

PTM databases

iPTMnetiO23647

Expressioni

Tissue specificityi

Mostly expressed in roots, stems, seeds, inflorescences, flowers and leaves, and, to a lower extent, in seedlings.2 Publications

Inductioni

Induced by light, preferentially when associated with glucose, fructose or sucrose treatment, but repressed by darkness.1 Publication

Gene expression databases

ExpressionAtlasiO23647 baseline and differential
GenevisibleiO23647 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi3556, 1 interactor
STRINGi3702.AT2G36390.1

Structurei

3D structure databases

ProteinModelPortaliO23647
SMRiO23647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi838 – 843Poly-Asp6

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0470 Eukaryota
COG0296 LUCA
HOGENOMiHOG000175159
InParanoidiO23647
KOiK00700
OMAiRIGGFWE
OrthoDBiEOG0936025M
PhylomeDBiO23647

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYTISGVRF PHLPSIKKKN SSLHSFNEDL RRSNAVSFSL RKDSRSSGKV
60 70 80 90 100
FARKPSYDSD SSSLATTASE KLRGHQSDSS SSASDQVQSR DTVSDDTQVL
110 120 130 140 150
GNVDVQKTEE AQETETLDQT SALSTSGSIS YKEDFAKMSH SVDQEVGQRK
160 170 180 190 200
IPPPGDGKRI YDIDPMLNSH RNHLDYRYGQ YRKLREEIDK NEGGLEAFSR
210 220 230 240 250
GYEIFGFTRS ATGITYREWA PGAKAASLIG DFNNWNAKSD VMARNDFGVW
260 270 280 290 300
EIFLPNNADG SPAIPHGSRV KIRMDTPSGI KDSIPAWIKY SVQPPGEIPY
310 320 330 340 350
NGVYYDPPEE DKYAFKHPRP KKPTSLRIYE SHVGMSSTEP KINTYANFRD
360 370 380 390 400
DVLPRIKKLG YNAVQIMAIQ EHAYYASFGY HVTNFFAPSS RFGTPDDLKS
410 420 430 440 450
LIDKAHELGL VVLMDIVHSH ASKNTLDGLD MFDGTDGQYF HSGSRGYHWM
460 470 480 490 500
WDSRLFNYGS WEVLRYLLSN ARWWLEEYKF DGFRFDGVTS MMYTHHGLQV
510 520 530 540 550
EFTGNYNEYF GYSTDVDAVV YLMLVNDLIH GLYPEAIVVG EDVSGMPAFC
560 570 580 590 600
VPVEDGGVGF DYRLHMAVAD KWIELLKKRD EDWQVGDITF TLTNRRWGEK
610 620 630 640 650
CVVYAESHDQ ALVGDKTIAF WLMDKDMYDF MAVDRQATPR VDRGIALHKM
660 670 680 690 700
IRLITMGLGG EGYLNFMGNE FGHPEWIDFP RTDQHLPDGR VIAGNNGSYD
710 720 730 740 750
KCRRRFDLGD AEYLRYHGLQ EFDRAMQNLE ETYGFMTSEH QYISRKDEGD
760 770 780 790 800
RVIVFERGNL LFVFNFHWTN SYSDYRIGCS VPGKYKIVLD SDNSLFGGFN
810 820 830 840 850
RLDDSAEFFT SDGRHDDRPC SFMVYAPCRT AVVYAAVDDD DDDERSSLVP

IGLLPEDV
Length:858
Mass (Da):97,660
Last modified:January 1, 1998 - v1
Checksum:iE1D61C0C21D456F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 7ISG → RAR in AAB03099 (PubMed:8616246).Curated3
Sequence conflicti453S → T in AAB03099 (PubMed:8616246).Curated1
Sequence conflicti702C → S in AAB03099 (PubMed:8616246).Curated1
Sequence conflicti745R → P in AAB03099 (PubMed:8616246).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000497 Genomic DNA Translation: CAA04134.1
AC006919 Genomic DNA Translation: AAD24644.1
CP002685 Genomic DNA Translation: AEC09247.1
AY136411 mRNA Translation: AAM97077.1
AK226896 mRNA Translation: BAE98973.1
U18817 mRNA Translation: AAB03099.1
PIRiB84780
S65045
RefSeqiNP_181180.1, NM_129196.4
UniGeneiAt.24317
At.67284

Genome annotation databases

EnsemblPlantsiAT2G36390.1; AT2G36390.1; AT2G36390
GeneIDi818212
GrameneiAT2G36390.1; AT2G36390.1; AT2G36390
KEGGiath:AT2G36390

Similar proteinsi

Entry informationi

Entry nameiGLGB1_ARATH
AccessioniPrimary (citable) accession number: O23647
Secondary accession number(s): Q42526
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health