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O23647

- GLGB1_ARATH

UniProt

O23647 - GLGB1_ARATH

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Protein
1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic
Gene
SBE2.1, BE3, At2g36390, F1O11.2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei486 – 4861Nucleophile By similarity
Active sitei541 – 5411Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: TAIR
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. amylopectin biosynthetic process Source: TAIR
  2. cellular response to fructose stimulus Source: UniProtKB
  3. cellular response to glucose stimulus Source: UniProtKB
  4. cellular response to light stimulus Source: UniProtKB
  5. cellular response to sucrose stimulus Source: UniProtKB
  6. glycogen biosynthetic process Source: InterPro
  7. starch biosynthetic process Source: UniProtKB-UniPathway
  8. starch metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G36390-MONOMER.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic (EC:2.4.1.18)
Short name:
AtSBE II-1
Alternative name(s):
Branching enzyme 3
Short name:
AtBE3
Starch-branching enzyme 2-1
Gene namesi
Name:SBE2.1
Synonyms:BE3
Ordered Locus Names:At2g36390
ORF Names:F1O11.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G36390.

Subcellular locationi

Plastidchloroplast stroma. Plastidamyloplast By similarity 4 Publications

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Modified starch composition. This phenotype is enhanced when associated with SBE2.2 and SBE3 disruptions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737Chloroplast Reviewed prediction
Add
BLAST
Chaini38 – 8588211,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic
PRO_0000415335Add
BLAST

Proteomic databases

PaxDbiO23647.
PRIDEiO23647.

Expressioni

Tissue specificityi

Mostly expressed in roots, stems, seeds, inflorescences, flowers and leaves, and, to a lower extent, in seedlings.2 Publications

Inductioni

Induced by light, preferentially when associated with glucose, fructose or sucrose treatment, but repressed by darkness.1 Publication

Gene expression databases

GenevestigatoriO23647.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi3702.AT2G36390.1-P.

Structurei

3D structure databases

ProteinModelPortaliO23647.
SMRiO23647. Positions 177-838.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi838 – 8436Poly-Asp

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
InParanoidiO23647.
KOiK00700.
OMAiNEVIHFL.
PhylomeDBiO23647.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23647-1 [UniParc]FASTAAdd to Basket

« Hide

MVYTISGVRF PHLPSIKKKN SSLHSFNEDL RRSNAVSFSL RKDSRSSGKV    50
FARKPSYDSD SSSLATTASE KLRGHQSDSS SSASDQVQSR DTVSDDTQVL 100
GNVDVQKTEE AQETETLDQT SALSTSGSIS YKEDFAKMSH SVDQEVGQRK 150
IPPPGDGKRI YDIDPMLNSH RNHLDYRYGQ YRKLREEIDK NEGGLEAFSR 200
GYEIFGFTRS ATGITYREWA PGAKAASLIG DFNNWNAKSD VMARNDFGVW 250
EIFLPNNADG SPAIPHGSRV KIRMDTPSGI KDSIPAWIKY SVQPPGEIPY 300
NGVYYDPPEE DKYAFKHPRP KKPTSLRIYE SHVGMSSTEP KINTYANFRD 350
DVLPRIKKLG YNAVQIMAIQ EHAYYASFGY HVTNFFAPSS RFGTPDDLKS 400
LIDKAHELGL VVLMDIVHSH ASKNTLDGLD MFDGTDGQYF HSGSRGYHWM 450
WDSRLFNYGS WEVLRYLLSN ARWWLEEYKF DGFRFDGVTS MMYTHHGLQV 500
EFTGNYNEYF GYSTDVDAVV YLMLVNDLIH GLYPEAIVVG EDVSGMPAFC 550
VPVEDGGVGF DYRLHMAVAD KWIELLKKRD EDWQVGDITF TLTNRRWGEK 600
CVVYAESHDQ ALVGDKTIAF WLMDKDMYDF MAVDRQATPR VDRGIALHKM 650
IRLITMGLGG EGYLNFMGNE FGHPEWIDFP RTDQHLPDGR VIAGNNGSYD 700
KCRRRFDLGD AEYLRYHGLQ EFDRAMQNLE ETYGFMTSEH QYISRKDEGD 750
RVIVFERGNL LFVFNFHWTN SYSDYRIGCS VPGKYKIVLD SDNSLFGGFN 800
RLDDSAEFFT SDGRHDDRPC SFMVYAPCRT AVVYAAVDDD DDDERSSLVP 850
IGLLPEDV 858
Length:858
Mass (Da):97,660
Last modified:January 1, 1998 - v1
Checksum:iE1D61C0C21D456F1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 73ISG → RAR in AAB03099. 1 Publication
Sequence conflicti453 – 4531S → T in AAB03099. 1 Publication
Sequence conflicti702 – 7021C → S in AAB03099. 1 Publication
Sequence conflicti745 – 7451R → P in AAB03099. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000497 Genomic DNA. Translation: CAA04134.1.
AC006919 Genomic DNA. Translation: AAD24644.1.
CP002685 Genomic DNA. Translation: AEC09247.1.
AY136411 mRNA. Translation: AAM97077.1.
AK226896 mRNA. Translation: BAE98973.1.
U18817 mRNA. Translation: AAB03099.1.
PIRiB84780.
S65045.
RefSeqiNP_181180.1. NM_129196.3.
UniGeneiAt.24317.
At.67284.

Genome annotation databases

EnsemblPlantsiAT2G36390.1; AT2G36390.1; AT2G36390.
GeneIDi818212.
KEGGiath:AT2G36390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000497 Genomic DNA. Translation: CAA04134.1 .
AC006919 Genomic DNA. Translation: AAD24644.1 .
CP002685 Genomic DNA. Translation: AEC09247.1 .
AY136411 mRNA. Translation: AAM97077.1 .
AK226896 mRNA. Translation: BAE98973.1 .
U18817 mRNA. Translation: AAB03099.1 .
PIRi B84780.
S65045.
RefSeqi NP_181180.1. NM_129196.3.
UniGenei At.24317.
At.67284.

3D structure databases

ProteinModelPortali O23647.
SMRi O23647. Positions 177-838.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G36390.1-P.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbi O23647.
PRIDEi O23647.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G36390.1 ; AT2G36390.1 ; AT2G36390 .
GeneIDi 818212.
KEGGi ath:AT2G36390.

Organism-specific databases

TAIRi AT2G36390.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000175159.
InParanoidi O23647.
KOi K00700.
OMAi NEVIHFL.
PhylomeDBi O23647.

Enzyme and pathway databases

UniPathwayi UPA00152 .
BioCyci ARA:AT2G36390-MONOMER.

Gene expression databases

Genevestigatori O23647.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential accumulation of Arabidopsis thaliana Sbe 2.1 and Sbe 2.2 transcripts in response to light."
    Khoshnoodi J., Larsson C.-T., Larsson H., Rask L.
    Plant Sci. 135:183-193(1998)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Two closely related cDNAs encoding starch branching enzyme from Arabidopsis thaliana."
    Fisher D.K., Gao M., Kim K.-N., Boyer C.D., Guiltinan M.J.
    Plant Mol. Biol. 30:97-108(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-858, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
    Tissue: Seedling hypocotyl.
  7. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
    Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
    Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation."
    Dumez S., Wattebled F., Dauvillee D., Delvalle D., Planchot V., Ball S.G., D'Hulst C.
    Plant Cell 18:2694-2709(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia and cv. Wassilewskija.
  9. "Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants."
    Han Y., Sun F.-J., Rosales-Mendoza S., Korban S.S.
    Gene 401:123-130(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  10. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  11. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Wassilewskija.
  12. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiGLGB1_ARATH
AccessioniPrimary (citable) accession number: O23647
Secondary accession number(s): Q42526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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