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Protein

1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic

Gene

SBE2.1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei486NucleophileBy similarity1
Active sitei541Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G36390-MONOMER.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic (EC:2.4.1.18)
Short name:
AtSBE II-1
Alternative name(s):
Branching enzyme 3
Short name:
AtBE3
Starch-branching enzyme 2-1
Gene namesi
Name:SBE2.1
Synonyms:BE3
Ordered Locus Names:At2g36390
ORF Names:F1O11.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36390.

Subcellular locationi

GO - Cellular componenti

  • amyloplast Source: UniProtKB-SubCell
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Modified starch composition. This phenotype is enhanced when associated with SBE2.2 and SBE3 disruptions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37ChloroplastSequence analysisAdd BLAST37
ChainiPRO_000041533538 – 8581,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplasticAdd BLAST821

Proteomic databases

PaxDbiO23647.

PTM databases

iPTMnetiO23647.

Expressioni

Tissue specificityi

Mostly expressed in roots, stems, seeds, inflorescences, flowers and leaves, and, to a lower extent, in seedlings.2 Publications

Inductioni

Induced by light, preferentially when associated with glucose, fructose or sucrose treatment, but repressed by darkness.1 Publication

Gene expression databases

GenevisibleiO23647. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi3556. 1 interactor.
STRINGi3702.AT2G36390.1.

Structurei

3D structure databases

ProteinModelPortaliO23647.
SMRiO23647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi838 – 843Poly-Asp6

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0470. Eukaryota.
COG0296. LUCA.
HOGENOMiHOG000175159.
InParanoidiO23647.
KOiK00700.
OMAiTPNDHAR.
OrthoDBiEOG0936025M.
PhylomeDBiO23647.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYTISGVRF PHLPSIKKKN SSLHSFNEDL RRSNAVSFSL RKDSRSSGKV
60 70 80 90 100
FARKPSYDSD SSSLATTASE KLRGHQSDSS SSASDQVQSR DTVSDDTQVL
110 120 130 140 150
GNVDVQKTEE AQETETLDQT SALSTSGSIS YKEDFAKMSH SVDQEVGQRK
160 170 180 190 200
IPPPGDGKRI YDIDPMLNSH RNHLDYRYGQ YRKLREEIDK NEGGLEAFSR
210 220 230 240 250
GYEIFGFTRS ATGITYREWA PGAKAASLIG DFNNWNAKSD VMARNDFGVW
260 270 280 290 300
EIFLPNNADG SPAIPHGSRV KIRMDTPSGI KDSIPAWIKY SVQPPGEIPY
310 320 330 340 350
NGVYYDPPEE DKYAFKHPRP KKPTSLRIYE SHVGMSSTEP KINTYANFRD
360 370 380 390 400
DVLPRIKKLG YNAVQIMAIQ EHAYYASFGY HVTNFFAPSS RFGTPDDLKS
410 420 430 440 450
LIDKAHELGL VVLMDIVHSH ASKNTLDGLD MFDGTDGQYF HSGSRGYHWM
460 470 480 490 500
WDSRLFNYGS WEVLRYLLSN ARWWLEEYKF DGFRFDGVTS MMYTHHGLQV
510 520 530 540 550
EFTGNYNEYF GYSTDVDAVV YLMLVNDLIH GLYPEAIVVG EDVSGMPAFC
560 570 580 590 600
VPVEDGGVGF DYRLHMAVAD KWIELLKKRD EDWQVGDITF TLTNRRWGEK
610 620 630 640 650
CVVYAESHDQ ALVGDKTIAF WLMDKDMYDF MAVDRQATPR VDRGIALHKM
660 670 680 690 700
IRLITMGLGG EGYLNFMGNE FGHPEWIDFP RTDQHLPDGR VIAGNNGSYD
710 720 730 740 750
KCRRRFDLGD AEYLRYHGLQ EFDRAMQNLE ETYGFMTSEH QYISRKDEGD
760 770 780 790 800
RVIVFERGNL LFVFNFHWTN SYSDYRIGCS VPGKYKIVLD SDNSLFGGFN
810 820 830 840 850
RLDDSAEFFT SDGRHDDRPC SFMVYAPCRT AVVYAAVDDD DDDERSSLVP

IGLLPEDV
Length:858
Mass (Da):97,660
Last modified:January 1, 1998 - v1
Checksum:iE1D61C0C21D456F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 7ISG → RAR in AAB03099 (PubMed:8616246).Curated3
Sequence conflicti453S → T in AAB03099 (PubMed:8616246).Curated1
Sequence conflicti702C → S in AAB03099 (PubMed:8616246).Curated1
Sequence conflicti745R → P in AAB03099 (PubMed:8616246).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000497 Genomic DNA. Translation: CAA04134.1.
AC006919 Genomic DNA. Translation: AAD24644.1.
CP002685 Genomic DNA. Translation: AEC09247.1.
AY136411 mRNA. Translation: AAM97077.1.
AK226896 mRNA. Translation: BAE98973.1.
U18817 mRNA. Translation: AAB03099.1.
PIRiB84780.
S65045.
RefSeqiNP_181180.1. NM_129196.4.
UniGeneiAt.24317.
At.67284.

Genome annotation databases

EnsemblPlantsiAT2G36390.1; AT2G36390.1; AT2G36390.
GeneIDi818212.
GrameneiAT2G36390.1; AT2G36390.1; AT2G36390.
KEGGiath:AT2G36390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000497 Genomic DNA. Translation: CAA04134.1.
AC006919 Genomic DNA. Translation: AAD24644.1.
CP002685 Genomic DNA. Translation: AEC09247.1.
AY136411 mRNA. Translation: AAM97077.1.
AK226896 mRNA. Translation: BAE98973.1.
U18817 mRNA. Translation: AAB03099.1.
PIRiB84780.
S65045.
RefSeqiNP_181180.1. NM_129196.4.
UniGeneiAt.24317.
At.67284.

3D structure databases

ProteinModelPortaliO23647.
SMRiO23647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3556. 1 interactor.
STRINGi3702.AT2G36390.1.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiO23647.

Proteomic databases

PaxDbiO23647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36390.1; AT2G36390.1; AT2G36390.
GeneIDi818212.
GrameneiAT2G36390.1; AT2G36390.1; AT2G36390.
KEGGiath:AT2G36390.

Organism-specific databases

TAIRiAT2G36390.

Phylogenomic databases

eggNOGiKOG0470. Eukaryota.
COG0296. LUCA.
HOGENOMiHOG000175159.
InParanoidiO23647.
KOiK00700.
OMAiTPNDHAR.
OrthoDBiEOG0936025M.
PhylomeDBiO23647.

Enzyme and pathway databases

UniPathwayiUPA00152.
BioCyciARA:AT2G36390-MONOMER.

Miscellaneous databases

PROiO23647.

Gene expression databases

GenevisibleiO23647. AT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGB1_ARATH
AccessioniPrimary (citable) accession number: O23647
Secondary accession number(s): Q42526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.