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Reviewed, UniProtKB/Swiss-Prot O23627 (SYGM1_ARATH)

Last modified November 3, 2009. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glycyl-tRNA synthetase 1, mitochondrial
    EC=6.1.1.14
Alternative name(s):
    Glycine--tRNA ligase 1
      Short name=GlyRS 1
Gene names
Name: GLYRS-1
Ordered Locus Names: At1g29880
ORF Names: F1N18.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion. Cytoplasm. Ref.5

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Contains 1 WHEP-TRS domain.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
Mitochondrion
   Coding sequence diversityAlternative initiation
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

response to cadmium ion

Inferred from expression pattern. Source: TAIR

   Cellular componentmitochondrion

Inferred from direct assay. Source: TAIR

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glycine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Mitochondrial (identifier: O23627-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Cytoplasmic (identifier: O23627-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2828Mitochondrion Potential
Chain29 – 729701Glycyl-tRNA synthetase 1, mitochondrial
PRO_0000035802

Regions

Domain50 – 10657WHEP-TRS

Natural variations

Alternative sequence1 – 3939Missing in isoform Cytoplasmic.
VSP_018906

Experimental info

Sequence conflict11M → T Ref.4
Sequence conflict821I → M in AAK92832. Ref.3
Sequence conflict6321V → A in AAM64494. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Isoform Mitochondrial [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: BFF40B27571794E6

FASTA72981,944
        10         20         30         40         50         60 
MRIFSTFVFH RRQQIFNLRQ FQTTTILRNP ISIAPIQIPM DATEQSLRQS LSEKSSSVEA 

        70         80         90        100        110        120 
QGNAVRALKA SRAAKPEIDA AIEQLNKLKL EKSTVEKELQ SIISSSGNGS LNREAFRKAV 

       130        140        150        160        170        180 
VNTLERRLFY IPSFKIYSGV AGLFDYGPPG CAIKSNVLSF WRQHFILEEN MLEVDCPCVT 

       190        200        210        220        230        240 
PEVVLKASGH VDKFTDLMVK DEKTGTCYRA DHLLKDYCTE KLEKDLTISA EKAAELKDVL 

       250        260        270        280        290        300 
AVMEDFSPEQ LGAKIREYGI TAPDTKNPLS DPYPFNLMFQ TSIGPSGLIP GYMRPETAQG 

       310        320        330        340        350        360 
IFVNFKDLYY YNGKKLPFAA AQIGQAFRNE ISPRQGLLRV REFTLAEIEH FVDPENKSHP 

       370        380        390        400        410        420 
KFSDVAKLEF LMFPREEQMS GQSAKKLCLG EAVAKGTVNN ETLGYFIGRV YLFLTRLGID 

       430        440        450        460        470        480 
KERLRFRQHL ANEMAHYAAD CWDAEIESSY GWIECVGIAD RSAYDLRAHS DKSGTPLVAE 

       490        500        510        520        530        540 
EKFAEPKEVE KLVITPVKKE LGLAFKGNQK NVVESLEAMN EEEAMEMKAT LESKGEVEFY 

       550        560        570        580        590        600 
VCTLKKSVNI KKNMVSISKE KKKEHQRVFT PSVIEPSFGI GRIIYCLYEH CFSTRPSKAG 

       610        620        630        640        650        660 
DEQLNLFRFP PLVAPIKCTV FPLVQNQQFE EVAKVISKEL ASVGISHKID ITGTSIGKRY 

       670        680        690        700        710        720 
ARTDELGVPF AITVDSDTSV TIRERDSKDQ VRVTLKEAAS VVSSVSEGKM TWQDVWATFP 


HHSSAAADE

« Hide

Isoform Cytoplasmic.

Checksum: 705B4179AF455F3E
Show »

FASTA69077,231

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References

« Hide 'large scale' references
[1]"Isolation and characterization of a glycyl-tRNA synthetase sequence from Arabidopsis thaliana."
Duchene A.-M., Dietrich A.
Plant Gene Register PGR97-183
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Overlapping destinations for two dual targeted glycyl-tRNA synthetases in Arabidopsis thaliana and Phaseolus vulgaris."
Duchene A.-M., Peeters N., Dietrich A., Cosset A., Small I.D., Wintz H.
J. Biol. Chem. 276:15275-15283(2001) [PubMed: 11278923] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AJ002062 mRNA. Translation: CAA05162.1.
AC008030 Genomic DNA. Translation: AAG10608.1.
AY050895 mRNA. Translation: AAK92832.1.
AY086930 mRNA. Translation: AAM64494.1. Different initiation.
IPIIPI00528368.
IPI00760319.
PIRD86422.
RefSeqNP_564337.1.
UniGeneAt.73

3D structure databases

HSSPHSSP built from PDB template 1FYJ based on UniProtKB P07814.
ModBaseSearch...

Protein-protein interaction databases

STRINGO23627.

Proteomic databases

PRIDEO23627.

Genome annotation databases

GeneID839866.
GenomeReviewsGene locus AT1G29880 in contig CT485782_GR.
KEGGath:AT1G29880.
NMPDRfig|3702.1.peg.3322.

Organism-specific databases

GeneFarm2677. 262.
TAIRAt1g29880.

Phylogenomic databases

OMAAYRNEIS.

Enzyme and pathway databases

BRENDA6.1.1.14. 302.

Gene expression databases

ArrayExpressO23627.
GenevestigatorO23627.
GermOnlineAT1G29880. Arabidopsis thaliana.

Family and domain databases

InterProIPR002314. aa-tRNA-synt_IIb_cons-reg.
IPR006195. aa-tRNA-synth_II_cons-reg.
IPR004154. Anticodon_bd.
IPR002315. tRNA-synt_gly.
IPR018160. tRNA-synth_gly_a2dimer_C.
IPR000738. WHEP-TRS.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHERPTHR10745. tRNA-synt_gly. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSPR01043. TRNASYNTHGLY.
TIGRFAMsTIGR00389. glyS_dimeric. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSYGM1_ARATH
AccessionPrimary (citable) accession number: O23627
Secondary accession number(s): Q8LBY0, Q949T7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 3, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents