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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 5

Gene

TPS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 5 (EC:2.4.1.15)
Alternative name(s):
Trehalose-6-phosphate synthase 5
Short name:
AtTPS5
Gene namesi
Name:TPS5
Ordered Locus Names:At4g17770
ORF Names:dl4920W, FCAALL.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G17770.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi5 – 51S → A: Abolishes binding to GRF/14-3-3. 1 Publication
Mutagenesisi32 – 321T → A: Abolishes binding to GRF/14-3-3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 5PRO_0000324826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphoserine1 Publication
Modified residuei32 – 321Phosphothreonine1 Publication

Post-translational modificationi

Both Ser-5 and Thr-32 must be phosphorylated for binding to GRF/14-3-3.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO23617.
PRIDEiO23617.

PTM databases

iPTMnetiO23617.

Expressioni

Tissue specificityi

Low expression in leaves, stems, flower buds, flowers and siliques.1 Publication

Inductioni

90-fold induction by sucrose after 24 hours and by heat stress.2 Publications

Gene expression databases

ExpressionAtlasiO23617. baseline and differential.
GenevisibleiO23617. AT.

Interactioni

Subunit structurei

Binds to the phosphopeptide-binding site of GRF/14-3-3 and to MBF1c.

Protein-protein interaction databases

BioGridi12791. 2 interactions.
STRINGi3702.AT4G17770.1.

Structurei

3D structure databases

ProteinModelPortaliO23617.
SMRiO23617. Positions 144-546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 546487GlycosyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
In the C-terminal section; belongs to the trehalose phosphatase family.Curated

Phylogenomic databases

eggNOGiKOG1050. Eukaryota.
COG0380. LUCA.
COG1877. LUCA.
HOGENOMiHOG000191476.
InParanoidiO23617.
KOiK16055.
OMAiREKKRFP.
OrthoDBiEOG093601RY.
PhylomeDBiO23617.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.

Sequencei

Sequence statusi: Complete.

O23617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRSYSNLL DLASGNFHSF SREKKRFPRV ATVTGVLSEL DDDNNSNSVC
60 70 80 90 100
SDAPSSVTQD RIIIVGNQLP IKSHRNSAGK LSFSWDNDSL LLQLKDGMRE
110 120 130 140 150
DMEVVYIGCL KEQIDTVEQD DVSQRLLENF KCVPAYIPPE LFTKYYHGFC
160 170 180 190 200
KQHLWPLFHY MLPLTPDLGG RFDRSLWQAY LSVNKIFADK VMEVISPDDD
210 220 230 240 250
FVWVHDYHLM VLPTFLRKRF NRVKLGFFLH SPFPSSEIYR TLPVRNELLR
260 270 280 290 300
ALLNADLIGF HTFDYARHFL SCCSRMLGLS YQSKRGTIGL EYYGRTVSIK
310 320 330 340 350
ILPVGIHISQ LQSILNLPET QTKVAELRDQ FLDQKVLLGV DDMDIFKGIS
360 370 380 390 400
LKLLAMEQLL TQHPEKRGRV VLVQIANPAR GRGKDVQEVQ SETEATVKRI
410 420 430 440 450
NEMFGRPGYQ PVVLIDTPLQ FFERIAYYVI AECCLVTAVR DGMNLIPYEY
460 470 480 490 500
IICRQGNPKL NETIGLDPSA AKKSMLVVSE FIGCSPSLSG AIRVNPWNID
510 520 530 540 550
AVTEAMDYAL IVSEAEKQMR HEKHHKYVST HDVAYWARSF IQDLERACGD
560 570 580 590 600
HVRKRCWGIG FGLGFRVVAL DPSFKKLSIE HIVSAYKRTK NRAILLDYDG
610 620 630 640 650
TMVQPGSIRT TPTRETIEIL NNLSSDPKNI VYLVSGKDRR TLTEWFSSCD
660 670 680 690 700
DLGLGAEHGY FIRPNDGTDW ETSSLVSGFE WKQIAEPVMR LYTETTDGST
710 720 730 740 750
IETKETALVW NYQFADPDFG SCQAKELMEH LESVLTNDPV SVKTGQQLVE
760 770 780 790 800
VKPQGVNKGL VAERLLTTMQ EKGKLLDFIL CVGDDRSDED MFEVIMSAKD
810 820 830 840 850
GPALSPVAEI FACTVGQKPS KAKYYLDDTA EIIRMLDGLA ATNTTISDQT
860
DSTATVPTKD LF
Length:862
Mass (Da):97,454
Last modified:March 18, 2008 - v2
Checksum:iADA155DB26DCB470
GO

Sequence cautioni

The sequence CAB10557 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB78780 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97344 Genomic DNA. Translation: CAB10557.1. Sequence problems.
AL161547 Genomic DNA. Translation: CAB78780.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83948.1.
BT005967 mRNA. Translation: AAO64902.1.
AK118703 mRNA. Translation: BAC43297.1.
PIRiH71447.
RefSeqiNP_567538.1. NM_117886.2.
UniGeneiAt.4501.

Genome annotation databases

EnsemblPlantsiAT4G17770.1; AT4G17770.1; AT4G17770.
GeneIDi827498.
GrameneiAT4G17770.1; AT4G17770.1; AT4G17770.
KEGGiath:AT4G17770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97344 Genomic DNA. Translation: CAB10557.1. Sequence problems.
AL161547 Genomic DNA. Translation: CAB78780.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83948.1.
BT005967 mRNA. Translation: AAO64902.1.
AK118703 mRNA. Translation: BAC43297.1.
PIRiH71447.
RefSeqiNP_567538.1. NM_117886.2.
UniGeneiAt.4501.

3D structure databases

ProteinModelPortaliO23617.
SMRiO23617. Positions 144-546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12791. 2 interactions.
STRINGi3702.AT4G17770.1.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

PTM databases

iPTMnetiO23617.

Proteomic databases

PaxDbiO23617.
PRIDEiO23617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G17770.1; AT4G17770.1; AT4G17770.
GeneIDi827498.
GrameneiAT4G17770.1; AT4G17770.1; AT4G17770.
KEGGiath:AT4G17770.

Organism-specific databases

TAIRiAT4G17770.

Phylogenomic databases

eggNOGiKOG1050. Eukaryota.
COG0380. LUCA.
COG1877. LUCA.
HOGENOMiHOG000191476.
InParanoidiO23617.
KOiK16055.
OMAiREKKRFP.
OrthoDBiEOG093601RY.
PhylomeDBiO23617.

Miscellaneous databases

PROiO23617.

Gene expression databases

ExpressionAtlasiO23617. baseline and differential.
GenevisibleiO23617. AT.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTPS5_ARATH
AccessioniPrimary (citable) accession number: O23617
Secondary accession number(s): Q8GWQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: September 7, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

2-deoxyglucose, but not phenformin, enhances the phosphorylation of TPS5.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.