Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O23553 (BAM3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-amylase 3, chloroplastic

EC=3.2.1.2
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 8
Chloroplast beta-amylase
Short name=CT-BMY
Gene names
Name:BAM3
Synonyms:BMY8, CTBMY
Ordered Locus Names:At4g17090
ORF Names:dl4575c, FCAALL.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Beta-amylase activity. No alpha-amylase activity. Involved in cold resistance. Mediates the accumulation of maltose upon freezing stress, thus contributing to the protection of the photosynthetic electron transport chain. Plays a role in the circadian-regulated starch degradation and maltose metabolism in chloroplasts, especially at night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan. Ref.1 Ref.7 Ref.8 Ref.11 Ref.12 Ref.13

Catalytic activity

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Enzyme regulation

Redox regulation; active in reducing conditions, inactive in oxidizing conditions By similarity.

Subcellular location

Plastidchloroplast Ref.1 Ref.9 Ref.12.

Tissue specificity

Expressed in vascular tissue of cotyledons, leaves, petioles, stems, petals, siliques and roots, particularly in phloem, as well as in photosynthetic tissues. Ref.14

Induction

By cold stress. Light-mediated circadian regulation; highest levels at dawn and at dusk in long days (LD) but only at dusk in short days (SD). Repressed by trehalose in a ABI4-dependent manner, this effect is reversed in the presence of sucrose. Ref.7 Ref.8 Ref.10

Disruption phenotype

Slightly retarded growth rate and reduced starch breakdown in leaves during the night. Ref.12

Sequence similarities

Belongs to the glycosyl hydrolase 14 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 6-7. Ref.12 Ref.13

Sequence caution

The sequence AAK96508.1 differs from that shown. Reason: Erroneous termination at position 377. Translated as Trp.

The sequence AAL31225.1 differs from that shown. Reason: Erroneous termination at position 377. Translated as Trp.

The sequence CAB46051.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAB80980.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Potential
Chain50 – 548499Beta-amylase 3, chloroplastic
PRO_0000393418

Regions

Region457 – 4582Substrate binding By similarity

Sites

Active site2591Proton donor By similarity
Active site4561Proton acceptor By similarity
Binding site1271Substrate By similarity
Binding site1671Substrate By similarity
Binding site1751Substrate By similarity
Binding site3711Substrate By similarity
Binding site3761Substrate By similarity
Binding site4181Substrate By similarity
Binding site4891Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O23553 [UniParc].

Last modified April 20, 2010. Version 3.
Checksum: A5794EDC8427FE30

FASTA54861,353
        10         20         30         40         50         60 
MELTLNSSSS LIKRKDAKSS RNQESSSNNM TFAKMKPPTY QFQAKNSVKE MKFTHEKTFT 

        70         80         90        100        110        120 
PEGETLEKWE KLHVLSYPHS KNDASVPVFV MLPLDTVTMS GHLNKPRAMN ASLMALKGAG 

       130        140        150        160        170        180 
VEGVMVDAWW GLVEKDGPMN YNWEGYAELI QMVQKHGLKL QVVMSFHQCG GNVGDSCSIP 

       190        200        210        220        230        240 
LPPWVLEEIS KNPDLVYTDK SGRRNPEYIS LGCDSVPVLR GRTPIQVYSD FMRSFRERFE 

       250        260        270        280        290        300 
GYIGGVIAEI QVGMGPCGEL RYPSYPESNG TWRFPGIGEF QCYDKYMKSS LQAYAESIGK 

       310        320        330        340        350        360 
TNWGTSGPHD AGEYKNLPED TEFFRRDGTW NSEYGKFFME WYSGKLLEHG DQLLSSAKGI 

       370        380        390        400        410        420 
FQGSGAKLSG KVAGIHWHYN TRSHAAELTA GYYNTRNHDG YLPIAKMFNK HGVVLNFTCM 

       430        440        450        460        470        480 
EMKDGEQPEH ANCSPEGLVK QVQNATRQAG TELAGENALE RYDSSAFGQV VATNRSDSGN 

       490        500        510        520        530        540 
GLTAFTYLRM NKRLFEGQNW QQLVEFVKNM KEGGHGRRLS KEDTTGSDLY VGFVKGKIAE 


NVEEAALV 

« Hide

References

« Hide 'large scale' references
[1]"An Arabidopsis gene encoding a chloroplast-targeted beta-amylase."
Lao N.T., Schoneveld O., Mould R.M., Hibberd J.M., Gray J.C., Kavanagh T.A.
Plant J. 20:519-527(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. expand/collapse author list , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"RNA interference of Arabidopsis beta-amylase8 prevents maltose accumulation upon cold shock and increases sensitivity of PSII photochemical efficiency to freezing stress."
Kaplan F., Guy C.L.
Plant J. 44:730-743(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY COLD.
[8]"Daylength and circadian effects on starch degradation and maltose metabolism."
Lu Y., Gehan J.P., Sharkey T.D.
Plant Physiol. 138:2280-2291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY CIRCADIAN DAYLENGTH.
[9]"Redox regulation of a novel plastid-targeted beta-amylase of Arabidopsis."
Sparla F., Costa A., Lo Schiavo F., Pupillo P., Trost P.
Plant Physiol. 141:840-850(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"ABI4 mediates the effects of exogenous trehalose on Arabidopsis growth and starch breakdown."
Ramon M., Rolland F., Thevelein J.M., van Dijck P., Leyman B.
Plant Mol. Biol. 63:195-206(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY TREHALOSE.
[11]"Glucan, water dikinase activity stimulates breakdown of starch granules by plastidial beta-amylases."
Edner C., Li J., Albrecht T., Mahlow S., Hejazi M., Hussain H., Kaplan F., Guy C., Smith S.M., Steup M., Ritte G.
Plant Physiol. 145:17-28(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts."
Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C.
Plant Cell 20:1040-1058(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE FAMILY, NOMENCLATURE.
[13]"Catalytically-inactive beta-amylase BAM4 required for starch breakdown in Arabidopsis leaves is a starch-binding-protein."
Li J., Francisco P., Zhou W., Edner C., Steup M., Ritte G., Bond C.S., Smith S.M.
Arch. Biochem. Biophys. 489:92-98(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[14]"The gene encoding the catalytically inactive beta-amylase BAM4 involved in starch breakdown in Arabidopsis leaves is expressed preferentially in vascular tissues in source and sink organs."
Francisco P., Li J., Smith S.M.
J. Plant Physiol. 167:890-895(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ250341 mRNA. Translation: CAB58423.1.
Z97342 Genomic DNA. Translation: CAB46051.1. Different initiation.
AL161545 Genomic DNA. Translation: CAB80980.1. Different initiation.
CP002687 Genomic DNA. Translation: AEE83848.1.
AY052315 mRNA. Translation: AAK96508.1. Sequence problems.
AY061898 mRNA. Translation: AAL31225.1. Sequence problems.
AY087592 mRNA. Translation: AAM65134.1.
PIRD71439.
H85190.
T52556.
RefSeqNP_567523.1. NM_117813.2.
UniGeneAt.22021.
At.23528.
At.47944.
At.67939.

3D structure databases

ProteinModelPortalO23553.
SMRO23553. Positions 86-510.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G17090.1-P.

Protein family/group databases

CAZyGH14. Glycoside Hydrolase Family 14.

Proteomic databases

PaxDbO23553.
PRIDEO23553.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G17090.1; AT4G17090.1; AT4G17090.
GeneID827419.
KEGGath:AT4G17090.

Organism-specific databases

TAIRAT4G17090.

Phylogenomic databases

eggNOGNOG77898.
HOGENOMHOG000238755.
InParanoidQ9SMW0.
KOK01177.
OMAEHANCSP.
PhylomeDBO23553.

Enzyme and pathway databases

BioCycARA:AT4G17090-MONOMER.
MetaCyc:AT4G17090-MONOMER.
BRENDA3.2.1.2. 399.

Gene expression databases

GenevestigatorO23553.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00506. BETA_AMYLASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBAM3_ARATH
AccessionPrimary (citable) accession number: O23553
Secondary accession number(s): Q941A5, Q9SMW0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 14, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names