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Protein

Probable pheophorbidase

Gene

PPD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the chlorophyll breakdown by its action in fluorescent chlorophyll catabolites (FCCs) demethylation. Demethylates the C132-carboxymethyl group present at the isocyclic ring of chlorophyll. Also able to catalyze pheophorbides in vitro. Methylesterase shown to have carboxylesterase activity, methyl indole-3-acetic acid (MeIAA) esterase activity and methyl jasmonate (MeJA) esterase activity in vitro.2 Publications

Catalytic activityi

Pheophorbide a + H2O = pyropheophorbide a + methanol + CO2.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Acyl-ester intermediateBy similarity1
Active sitei211Charge relay systemPROSITE-ProRule annotation1
Active sitei239Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB
  • hydrolase activity, acting on ester bonds Source: TAIR
  • methyl indole-3-acetate esterase activity Source: TAIR
  • methyl jasmonate esterase activity Source: TAIR
  • pheophoridase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • chlorophyll a catabolic process Source: UniProtKB
  • demethylation Source: UniProtKB
  • leaf senescence Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processChlorophyll catabolism

Enzyme and pathway databases

BioCyciARA:AT4G16690-MONOMER

Protein family/group databases

ESTHERiarath-AT4G16690 Hydroxynitrile_lyase

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pheophorbidase (EC:3.1.1.82)
Short name:
AtPPD
Alternative name(s):
FCC methylesterase
Methylesterase 16
Short name:
AtMES16
Gene namesi
Name:PPD
Synonyms:MES16
Ordered Locus Names:At4g16690
ORF Names:dl4370c, FCAALL.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G16690
TAIRilocus:2129041 AT4G16690

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Accumulation of fluorescent chlorophyll catabolites (FCC) during senescence. No effect on root length when grown in presence of exogenous MeIAA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003913491 – 262Probable pheophorbidaseAdd BLAST262

Proteomic databases

PaxDbiO23512

PTM databases

iPTMnetiO23512

Expressioni

Gene expression databases

ExpressionAtlasiO23512 baseline and differential
GenevisibleiO23512 AT

Interactioni

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi12664, 1 interactor
STRINGi3702.AT4G16690.1

Structurei

Secondary structure

1262
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 16Combined sources5
Helixi23 – 26Combined sources4
Helixi27 – 35Combined sources9
Beta strandi39 – 42Combined sources4
Helixi55 – 57Combined sources3
Helixi61 – 64Combined sources4
Helixi66 – 73Combined sources8
Beta strandi81 – 86Combined sources6
Helixi89 – 99Combined sources11
Helixi101 – 103Combined sources3
Beta strandi104 – 111Combined sources8
Beta strandi136 – 139Combined sources4
Beta strandi149 – 153Combined sources5
Helixi155 – 157Combined sources3
Helixi158 – 161Combined sources4
Helixi168 – 175Combined sources8
Helixi183 – 186Combined sources4
Helixi198 – 200Combined sources3
Beta strandi203 – 208Combined sources6
Beta strandi212 – 214Combined sources3
Helixi216 – 225Combined sources10
Beta strandi229 – 234Combined sources6
Helixi241 – 244Combined sources4
Helixi246 – 257Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HK8X-ray2.80A/B/C/D/E/F1-262[»]
ProteinModelPortaliO23512
SMRiO23512
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410JX5E Eukaryota
ENOG41104A6 LUCA
HOGENOMiHOG000133452
InParanoidiO23512
KOiK13544
OMAiVENWPPS
OrthoDBiEOG09360JHB
PhylomeDBiO23512

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
PfamiView protein in Pfam
PF12697 Abhydrolase_6, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

O23512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGEGGAEPV IHFVFVHGAS HGAWCWYKLT TLLDAAGFKS TSVDLTGAGI
60 70 80 90 100
SLIDSNIVFD SDQYNRPLFS LLSDLPPHHK VILVGHSIGG GSVTEALCKF
110 120 130 140 150
TDKISMAIYL AASMVQPGSI PSPHLSNIHV GEEDIWEYTY GEGTDKPPTG
160 170 180 190 200
VLMKPEFIRH YYYSQSPLED VTLSSKLLRP APMRAFQDLD KLPPNPEAEK
210 220 230 240 250
VPRVYIKTAK DNLFDSVRQD LLVENWPPSQ LYVLEDSDHS AFFSVPTTLF
260
AYLLRAVSFL QR
Length:262
Mass (Da):29,014
Last modified:January 1, 1998 - v1
Checksum:i5CAA518C1A05212D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97341 Genomic DNA Translation: CAB10444.1
AL161544 Genomic DNA Translation: CAB78711.1
CP002687 Genomic DNA Translation: AEE83784.1
AY088316 mRNA Translation: AAM65855.1
BX828808 mRNA No translation available.
PIRiB71434
RefSeqiNP_193402.1, NM_117770.5
UniGeneiAt.43149

Genome annotation databases

EnsemblPlantsiAT4G16690.1; AT4G16690.1; AT4G16690
GeneIDi827371
GrameneiAT4G16690.1; AT4G16690.1; AT4G16690
KEGGiath:AT4G16690

Similar proteinsi

Entry informationi

Entry nameiPPD_ARATH
AccessioniPrimary (citable) accession number: O23512
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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