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Protein

Metalloendoproteinase 1-MMP

Gene

1MMP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Matrix metalloproteinases (MMPs) or matrixins may play a role in the degradation and remodeling of the extracellular matrix (ECM) during development or in response to stresses (PubMed:10574937). Can cleave myelin basic protein as well as fluorigenic peptide substrates, McaPLANvaDpaAR-NH2 and McaPChaGNvaHADpa-NH2 4-fold more efficiently than McaPLGLDpaAR-NH2 (QF24) (PubMed:10574937). Active on myelin basic protein (MBP) and, to some extent, on McaPLGLDpaAR-NH2 (QF24) and beta-casein (PubMed:24156403).1 Publication2 Publications

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by human TIMP-1 and TIMP-2 and by the peptide hydroxamate inhibitor (BB-94) (PubMed:10574937). Repressed by acetohydroxamic acid (AHA) (PubMed:24156403).2 Publications

pH dependencei

Optimum pH is 7-8.1 Publication

Temperature dependencei

Optimum temperature is 45-55 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130Zinc 2; in inhibited formBy similarity1
Metal bindingi211Calcium 2By similarity1
Metal bindingi221Zinc 1By similarity1
Metal bindingi223Zinc 1By similarity1
Metal bindingi228Calcium 1By similarity1
Metal bindingi229Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi236Zinc 1By similarity1
Metal bindingi243Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi246Zinc 1By similarity1
Metal bindingi248Calcium 1By similarity1
Metal bindingi251Calcium 1By similarity1
Metal bindingi275Zinc 2; catalyticPROSITE-ProRule annotationBy similarity1
Active sitei276PROSITE-ProRule annotation1
Metal bindingi279Zinc 2; catalyticPROSITE-ProRule annotationBy similarity1
Metal bindingi285Zinc 2; catalyticPROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

  • metalloendopeptidase activity Source: UniProtKB
  • zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-1592389. Activation of Matrix Metalloproteinases.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiM10.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloendoproteinase 1-MMP1 Publication (EC:3.4.24.-Curated)
Short name:
At1-MMP1 Publication
Gene namesi
Name:1MMP1 Publication
Ordered Locus Names:At4g16640Imported
ORF Names:dl4345cImported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G16640.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • extracellular matrix Source: InterPro
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
PropeptideiPRO_000043351929 – 149Activation peptideBy similarityAdd BLAST121
ChainiPRO_0000433520150 – 339Metalloendoproteinase 1-MMPSequence analysisAdd BLAST190
PropeptideiPRO_0000433521340 – 364Removed in mature formSequence analysisAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi49N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi338N-linked (GlcNAc...)PROSITE-ProRule annotation1
Lipidationi339GPI-anchor amidated glycine1 Publication1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

PaxDbiO23507.

Expressioni

Tissue specificityi

Mostly expressed in flowers, roots and stems, and, to a lower extent, in leaves.1 Publication

Gene expression databases

GenevisibleiO23507. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G16640.1.

Structurei

3D structure databases

ProteinModelPortaliO23507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi128 – 135Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
HOGENOMiHOG000239471.
InParanoidiO23507.
OMAiHNATWHD.
OrthoDBiEOG09360MDE.
PhylomeDBiO23507.

Family and domain databases

CDDicd04278. ZnMc_MMP. 1 hit.
Gene3Di3.40.390.10. 1 hit.
InterProiIPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRNLIYRRN RALCFVLILF CFPYRFGARN TPEAEQSTAK ATQIIHVSNS
60 70 80 90 100
TWHDFSRLVD VQIGSHVSGV SELKRYLHRF GYVNDGSEIF SDVFDGPLES
110 120 130 140 150
AISLYQENLG LPITGRLDTS TVTLMSLPRC GVSDTHMTIN NDFLHTTAHY
160 170 180 190 200
TYFNGKPKWN RDTLTYAISK THKLDYLTSE DVKTVFRRAF SQWSSVIPVS
210 220 230 240 250
FEEVDDFTTA DLKIGFYAGD HGDGLPFDGV LGTLAHAFAP ENGRLHLDAA
260 270 280 290 300
ETWIVDDDLK GSSEVAVDLE SVATHEIGHL LGLGHSSQES AVMYPSLRPR
310 320 330 340 350
TKKVDLTVDD VAGVLKLYGP NPKLRLDSLT QSEDSIKNGT VSHRFLSGNF
360
IGYVLLVVGL ILFL
Length:364
Mass (Da):40,528
Last modified:January 1, 1998 - v1
Checksum:i844C5D612EAED662
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30N → I in AAO42162 (PubMed:14593172).Curated1
Sequence conflicti84N → K in AAM62476 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97341 Genomic DNA. Translation: CAB10439.1.
AL161544 Genomic DNA. Translation: CAB78706.1.
CP002687 Genomic DNA. Translation: AEE83779.1.
BT004141 mRNA. Translation: AAO42162.1.
AY085244 mRNA. Translation: AAM62476.1.
PIRiE71433.
RefSeqiNP_193397.1. NM_117765.4.
UniGeneiAt.33062.

Genome annotation databases

EnsemblPlantsiAT4G16640.1; AT4G16640.1; AT4G16640.
GeneIDi827365.
GrameneiAT4G16640.1; AT4G16640.1; AT4G16640.
KEGGiath:AT4G16640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97341 Genomic DNA. Translation: CAB10439.1.
AL161544 Genomic DNA. Translation: CAB78706.1.
CP002687 Genomic DNA. Translation: AEE83779.1.
BT004141 mRNA. Translation: AAO42162.1.
AY085244 mRNA. Translation: AAM62476.1.
PIRiE71433.
RefSeqiNP_193397.1. NM_117765.4.
UniGeneiAt.33062.

3D structure databases

ProteinModelPortaliO23507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G16640.1.

Protein family/group databases

MEROPSiM10.A04.

Proteomic databases

PaxDbiO23507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G16640.1; AT4G16640.1; AT4G16640.
GeneIDi827365.
GrameneiAT4G16640.1; AT4G16640.1; AT4G16640.
KEGGiath:AT4G16640.

Organism-specific databases

TAIRiAT4G16640.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
HOGENOMiHOG000239471.
InParanoidiO23507.
OMAiHNATWHD.
OrthoDBiEOG09360MDE.
PhylomeDBiO23507.

Enzyme and pathway databases

ReactomeiR-ATH-1592389. Activation of Matrix Metalloproteinases.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO23507.

Gene expression databases

GenevisibleiO23507. AT.

Family and domain databases

CDDicd04278. ZnMc_MMP. 1 hit.
Gene3Di3.40.390.10. 1 hit.
InterProiIPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei1MMP_ARATH
AccessioniPrimary (citable) accession number: O23507
Secondary accession number(s): Q84W77, Q8LET7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.