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Protein

Inositol transporter 4

Gene

INT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane inositol-proton symporter. Mediates high-affinity myoinositol-proton symport across the plasma membrane. Active with myoinositol, scylloinositol and D-chiroinositol. Low activity with mucoinositol and alloinositol.2 Publications

Kineticsi

Determined at three different membrane potentials: -90 mV, -40 mV and 0 mV, and at an extracellular pH of 5.5.

  1. KM=0.24 mM for myo-inositol1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-429593. Inositol transporters.

    Protein family/group databases

    TCDBi2.A.1.1.62. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol transporter 4
    Alternative name(s):
    Myo-inositol-proton symporter INT4
    Protein INOSITOL TRANSPORTER 4
    Gene namesi
    Name:INT4
    Ordered Locus Names:At4g16480
    ORF Names:dl4265w, FCAALL.375
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G16480.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5521Helical; Name=1Sequence analysisAdd
    BLAST
    Transmembranei70 – 9021Helical; Name=2Sequence analysisAdd
    BLAST
    Transmembranei105 – 12521Helical; Name=3Sequence analysisAdd
    BLAST
    Transmembranei128 – 14821Helical; Name=4Sequence analysisAdd
    BLAST
    Transmembranei162 – 18221Helical; Name=5Sequence analysisAdd
    BLAST
    Transmembranei188 – 20821Helical; Name=6Sequence analysisAdd
    BLAST
    Transmembranei290 – 31021Helical; Name=7Sequence analysisAdd
    BLAST
    Transmembranei317 – 33721Helical; Name=8Sequence analysisAdd
    BLAST
    Transmembranei345 – 36521Helical; Name=9Sequence analysisAdd
    BLAST
    Transmembranei456 – 47621Helical; Name=10Sequence analysisAdd
    BLAST
    Transmembranei494 – 51421Helical; Name=11Sequence analysisAdd
    BLAST
    Transmembranei525 – 54521Helical; Name=12Sequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi559 – 58224Missing : No effect on targeting. 1 PublicationAdd
    BLAST
    Mutagenesisi559 – 5613LLE → AAA: No effect on targeting. 1 Publication
    Mutagenesisi564 – 5652FK → AA: No effect on targeting. 1 Publication
    Mutagenesisi570 – 5756RRREKK → AAAAAA: No effect on targeting. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 582582Inositol transporter 4PRO_0000259878Add
    BLAST

    Proteomic databases

    PaxDbiO23492.
    PRIDEiO23492.

    Expressioni

    Tissue specificityi

    Highly expressed in pollen and phloem companion cells.1 Publication

    Gene expression databases

    GenevisibleiO23492. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi12639. 3 interactions.
    STRINGi3702.AT4G16480.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO23492.
    SMRiO23492. Positions 20-564.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The C-terminal domain (546-582) is required for plasma membrane targeting.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0254. Eukaryota.
    ENOG410XNQK. LUCA.
    HOGENOMiHOG000202868.
    InParanoidiO23492.
    KOiK08150.
    OMAiNATCSAY.
    OrthoDBiEOG093608AZ.
    PhylomeDBiO23492.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 2 hits.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O23492-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVEGGIAKAD KTEFTECWRT TWKTPYIMRL ALSAGIGGLL FGYDTGVISG
    60 70 80 90 100
    ALLFIKEDFD EVDKKTWLQS TIVSMAVAGA IVGAAVGGWI NDKFGRRMSI
    110 120 130 140 150
    LIADVLFLIG AIVMAFAPAP WVIIVGRIFV GFGVGMASMT SPLYISEASP
    160 170 180 190 200
    ARIRGALVST NGLLITGGQF FSYLINLAFV HTPGTWRWML GVAGVPAIVQ
    210 220 230 240 250
    FVLMLSLPES PRWLYRKDRI AESRAILERI YPADEVEAEM EALKLSVEAE
    260 270 280 290 300
    KADEAIIGDS FSAKLKGAFG NPVVRRGLAA GITVQVAQQF VGINTVMYYS
    310 320 330 340 350
    PSIVQFAGYA SNKTAMALSL ITSGLNALGS IVSMMFVDRY GRRKLMIISM
    360 370 380 390 400
    FGIIACLIIL ATVFSQAAIH APKIDAFESR TFAPNATCSA YAPLAAENAP
    410 420 430 440 450
    PSRWNCMKCL RSECGFCASG VQPYAPGACV VLSDDMKATC SSRGRTFFKD
    460 470 480 490 500
    GCPSKFGFLA IVFLGLYIVV YAPGMGTVPW IVNSEIYPLR YRGLGGGIAA
    510 520 530 540 550
    VSNWVSNLIV SESFLSLTHA LGSSGTFLLF AGFSTIGLFF IWLLVPETKG
    560 570 580
    LQFEEVEKLL EVGFKPSLLR RREKKGKEVD AA
    Length:582
    Mass (Da):62,892
    Last modified:January 1, 1998 - v1
    Checksum:iFA8F8DDCA5D0CBC0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ973178 mRNA. Translation: CAJ00306.1.
    Z97341 Genomic DNA. Translation: CAB10424.1.
    AL161544 Genomic DNA. Translation: CAB78690.1.
    CP002687 Genomic DNA. Translation: AEE83759.1.
    BT004139 mRNA. Translation: AAO42160.1.
    BT005707 mRNA. Translation: AAO64127.1.
    PIRiF71431.
    RefSeqiNP_193381.1. NM_117746.3.
    UniGeneiAt.33074.

    Genome annotation databases

    EnsemblPlantsiAT4G16480.1; AT4G16480.1; AT4G16480.
    GeneIDi827346.
    GrameneiAT4G16480.1; AT4G16480.1; AT4G16480.
    KEGGiath:AT4G16480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ973178 mRNA. Translation: CAJ00306.1.
    Z97341 Genomic DNA. Translation: CAB10424.1.
    AL161544 Genomic DNA. Translation: CAB78690.1.
    CP002687 Genomic DNA. Translation: AEE83759.1.
    BT004139 mRNA. Translation: AAO42160.1.
    BT005707 mRNA. Translation: AAO64127.1.
    PIRiF71431.
    RefSeqiNP_193381.1. NM_117746.3.
    UniGeneiAt.33074.

    3D structure databases

    ProteinModelPortaliO23492.
    SMRiO23492. Positions 20-564.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi12639. 3 interactions.
    STRINGi3702.AT4G16480.1.

    Protein family/group databases

    TCDBi2.A.1.1.62. the major facilitator superfamily (mfs).

    Proteomic databases

    PaxDbiO23492.
    PRIDEiO23492.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G16480.1; AT4G16480.1; AT4G16480.
    GeneIDi827346.
    GrameneiAT4G16480.1; AT4G16480.1; AT4G16480.
    KEGGiath:AT4G16480.

    Organism-specific databases

    TAIRiAT4G16480.

    Phylogenomic databases

    eggNOGiKOG0254. Eukaryota.
    ENOG410XNQK. LUCA.
    HOGENOMiHOG000202868.
    InParanoidiO23492.
    KOiK08150.
    OMAiNATCSAY.
    OrthoDBiEOG093608AZ.
    PhylomeDBiO23492.

    Enzyme and pathway databases

    ReactomeiR-ATH-429593. Inositol transporters.

    Miscellaneous databases

    PROiO23492.

    Gene expression databases

    GenevisibleiO23492. AT.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 2 hits.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiINT4_ARATH
    AccessioniPrimary (citable) accession number: O23492
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2006
    Last sequence update: January 1, 1998
    Last modified: September 7, 2016
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.