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Protein

Inositol transporter 4

Gene

INT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane inositol-proton symporter. Mediates high-affinity myoinositol-proton symport across the plasma membrane. Active with myoinositol, scylloinositol and D-chiroinositol. Low activity with mucoinositol and alloinositol.2 Publications

Kineticsi

Determined at three different membrane potentials: -90 mV, -40 mV and 0 mV, and at an extracellular pH of 5.5.
  1. KM=0.24 mM for myo-inositol1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Biological processSymport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-429593 Inositol transporters

    Protein family/group databases

    TCDBi2.A.1.1.62 the major facilitator superfamily (mfs)

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol transporter 4
    Alternative name(s):
    Myo-inositol-proton symporter INT4
    Protein INOSITOL TRANSPORTER 4
    Gene namesi
    Name:INT4
    Ordered Locus Names:At4g16480
    ORF Names:dl4265w, FCAALL.375
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G16480
    TAIRilocus:2130689 AT4G16480

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei35 – 55Helical; Name=1Sequence analysisAdd BLAST21
    Transmembranei70 – 90Helical; Name=2Sequence analysisAdd BLAST21
    Transmembranei105 – 125Helical; Name=3Sequence analysisAdd BLAST21
    Transmembranei128 – 148Helical; Name=4Sequence analysisAdd BLAST21
    Transmembranei162 – 182Helical; Name=5Sequence analysisAdd BLAST21
    Transmembranei188 – 208Helical; Name=6Sequence analysisAdd BLAST21
    Transmembranei290 – 310Helical; Name=7Sequence analysisAdd BLAST21
    Transmembranei317 – 337Helical; Name=8Sequence analysisAdd BLAST21
    Transmembranei345 – 365Helical; Name=9Sequence analysisAdd BLAST21
    Transmembranei456 – 476Helical; Name=10Sequence analysisAdd BLAST21
    Transmembranei494 – 514Helical; Name=11Sequence analysisAdd BLAST21
    Transmembranei525 – 545Helical; Name=12Sequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi559 – 582Missing : No effect on targeting. 1 PublicationAdd BLAST24
    Mutagenesisi559 – 561LLE → AAA: No effect on targeting. 1 Publication3
    Mutagenesisi564 – 565FK → AA: No effect on targeting. 1 Publication2
    Mutagenesisi570 – 575RRREKK → AAAAAA: No effect on targeting. 1 Publication6

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002598781 – 582Inositol transporter 4Add BLAST582

    Proteomic databases

    PaxDbiO23492
    PRIDEiO23492

    Expressioni

    Tissue specificityi

    Highly expressed in pollen and phloem companion cells.1 Publication

    Gene expression databases

    ExpressionAtlasiO23492 baseline and differential
    GenevisibleiO23492 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi12639, 3 interactors
    STRINGi3702.AT4G16480.1

    Structurei

    3D structure databases

    ProteinModelPortaliO23492
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The C-terminal domain (546-582) is required for plasma membrane targeting.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0254 Eukaryota
    ENOG410XNQK LUCA
    HOGENOMiHOG000202868
    InParanoidiO23492
    KOiK08150
    OMAiEWVEYEM
    OrthoDBiEOG093608AZ
    PhylomeDBiO23492

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR005828 MFS_sugar_transport-like
    IPR036259 MFS_trans_sf
    IPR003663 Sugar/inositol_transpt
    IPR005829 Sugar_transporter_CS
    PfamiView protein in Pfam
    PF00083 Sugar_tr, 2 hits
    PRINTSiPR00171 SUGRTRNSPORT
    SUPFAMiSSF103473 SSF103473, 2 hits
    TIGRFAMsiTIGR00879 SP, 1 hit
    PROSITEiView protein in PROSITE
    PS50850 MFS, 1 hit
    PS00216 SUGAR_TRANSPORT_1, 1 hit
    PS00217 SUGAR_TRANSPORT_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O23492-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVEGGIAKAD KTEFTECWRT TWKTPYIMRL ALSAGIGGLL FGYDTGVISG
    60 70 80 90 100
    ALLFIKEDFD EVDKKTWLQS TIVSMAVAGA IVGAAVGGWI NDKFGRRMSI
    110 120 130 140 150
    LIADVLFLIG AIVMAFAPAP WVIIVGRIFV GFGVGMASMT SPLYISEASP
    160 170 180 190 200
    ARIRGALVST NGLLITGGQF FSYLINLAFV HTPGTWRWML GVAGVPAIVQ
    210 220 230 240 250
    FVLMLSLPES PRWLYRKDRI AESRAILERI YPADEVEAEM EALKLSVEAE
    260 270 280 290 300
    KADEAIIGDS FSAKLKGAFG NPVVRRGLAA GITVQVAQQF VGINTVMYYS
    310 320 330 340 350
    PSIVQFAGYA SNKTAMALSL ITSGLNALGS IVSMMFVDRY GRRKLMIISM
    360 370 380 390 400
    FGIIACLIIL ATVFSQAAIH APKIDAFESR TFAPNATCSA YAPLAAENAP
    410 420 430 440 450
    PSRWNCMKCL RSECGFCASG VQPYAPGACV VLSDDMKATC SSRGRTFFKD
    460 470 480 490 500
    GCPSKFGFLA IVFLGLYIVV YAPGMGTVPW IVNSEIYPLR YRGLGGGIAA
    510 520 530 540 550
    VSNWVSNLIV SESFLSLTHA LGSSGTFLLF AGFSTIGLFF IWLLVPETKG
    560 570 580
    LQFEEVEKLL EVGFKPSLLR RREKKGKEVD AA
    Length:582
    Mass (Da):62,892
    Last modified:January 1, 1998 - v1
    Checksum:iFA8F8DDCA5D0CBC0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ973178 mRNA Translation: CAJ00306.1
    Z97341 Genomic DNA Translation: CAB10424.1
    AL161544 Genomic DNA Translation: CAB78690.1
    CP002687 Genomic DNA Translation: AEE83759.1
    CP002687 Genomic DNA Translation: ANM66112.1
    CP002687 Genomic DNA Translation: ANM66113.1
    BT004139 mRNA Translation: AAO42160.1
    BT005707 mRNA Translation: AAO64127.1
    PIRiF71431
    RefSeqiNP_001328028.1, NM_001341102.1
    NP_001328029.1, NM_001341103.1
    NP_193381.1, NM_117746.4
    UniGeneiAt.33074

    Genome annotation databases

    EnsemblPlantsiAT4G16480.1; AT4G16480.1; AT4G16480
    AT4G16480.2; AT4G16480.2; AT4G16480
    AT4G16480.3; AT4G16480.3; AT4G16480
    GeneIDi827346
    GrameneiAT4G16480.1; AT4G16480.1; AT4G16480
    AT4G16480.2; AT4G16480.2; AT4G16480
    AT4G16480.3; AT4G16480.3; AT4G16480
    KEGGiath:AT4G16480

    Similar proteinsi

    Entry informationi

    Entry nameiINT4_ARATH
    AccessioniPrimary (citable) accession number: O23492
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
    Last sequence update: January 1, 1998
    Last modified: May 23, 2018
    This is version 128 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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